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Enhancement of Erythropoietin Production from Chinese Hamster Ovary(CHO) Cells by Introduction of the Urea Cycle Enzymes, Carbamoyl Phosphate Synthetase I and Ornithine Transcarbamylase  

Kim, Na-Young (College of Pharmacy, Chung Ang University)
Lee, Yun-Jeong (College of Pharmacy, Chung Ang University)
Kim, Hyung-Jin (College of Pharmacy, Chung Ang University)
Choi, Jung-Ho (College of Pharmacy, Chung Ang University)
Kim, Jung-Kwon (College of Pharmacy, Chung Ang University)
Chang, Kern-Hee (Samsung Fine Chemicals Co., LTD. R&D Center)
Kim, Jung-Hoe (Department of Biological Science, KAIST)
Kim, Hong-Jin (College of Pharmacy, Chung Ang University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.4, 2004 , pp. 844-851 More about this Journal
Abstract
Efficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC), into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line, CO5, had a higher growth rate and accumulated less ammonia per cell than the parental cell line, IBE. In addition, it produced 2 times more EPO than the parent, and the purified EPO contained a higher proportion of acidic isoforms with approximately 15% more sialic acid.
Keywords
Erythropoietin; urea cycle enzymes; ammonia concentration; sialylation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
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