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Characterization and Identification of Bacillus thuringiensis subsp. tenebrionis SR6 and SR8

Kim, Il-Gi (Department of Genetic Engineering, Hallym University)
Lee, Jae-Wook (Department of Genetic Engineering, Hallym University)
Suh, Suk-Chul (National Institute of Agricultural Biotechnology, Rural Development Administration)
Rhim, Seong-Lyul (Department of Genetic Engineering, Hallym University)

Publication Information

Journal of Microbiology and Biotechnology / v.14, no.4, 2004 , pp. 772-776 More about this Journal

Abstract

Physiological and molecular characteristics of Bacillus thuringiensis SR6 and SR8 were investigated, and phase contrast and electron microscopies revealed that a large rhomboidal crystal protein was present in the sporulating cells. SDS-PAGE and Western blot analyses showed that B. thuringiensis SR8 produced 70 kDa protein much more than other proteins, and that the 70 kDa protein could bind to the antibody of B. thuringiensis subsp. tenebrionis-crystal toxin protein, indicating that the crystal 70 kDa protein has an immunological homology with B. thuringiensis subsp. tenebrionis-crystal toxin protein. The DNA fragment of B. thuringiensis subsp. tenebrionis-toxin gene was detected in B. thuringiensis SR6 and SR8 by using PCR amplification analysis. Furthermore, the insect bioassay showed the insecticidal activity against Colorado potato beetle larvae. Based on the physiological and molecular similarities to B. thuringiensis subsp. tenebrionis, it is suggested that the B. thuringiensis SR6 and SR8 may be mutants of the B. thuringiensis subsp. tenebrionis strain overexpressing the crystal of 70 kDa toxin protein.

Keywords

Bacillus thuringiensis; crystal protein; coleoptera;

Citations & Related Records

Times Cited By KSCI : 4 (Citation Analysis)
Times Cited By Web Of Science : 1 (Related Records In Web of Science)

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