Browse > Article

Comparison of Properties of Polyclonal Anti-N-Acetylchitooligosaccharides and Anti-Chitooligosaccharides Antibodies Produced for ELISA  

Shim, Youn-Young (Korea Food Research Institute/Graduate School of Biotechnology, Korea University)
Shon, Dong-Hwa (Korea Food Research Institute)
Kwak, Bo-Yeon (Korea Food Research Institute)
Yu, Jae-Hoon (Life Science Division, Korea Institute of Science and Technology)
Chee, Kew-Mahn (Graduate School of Biotechnology, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.4, 2004 , pp. 686-692 More about this Journal
Abstract
To develop the enzyme-linked immunosorbent assay (ELISA) for the analysis of N-acetylehitooligosaccharides (NACOS) and chitooligosaccharides (COS), specific antibodies (Abs) were produced, and their properties were compared. N-acetylehitohexaose (NACOS6), chitohexaose (COS6), and COS mixture (COSM) conjugated to bovine serum albumin (BSA) were used to immunize rabbits. By the use of specific Abs and NACOS6-horseradish peroxidase (HRP), COS6-HRP, and COSM-HRP conjugates, competitive direct ELISA (cdELISA) was developed. The detection limits of NACOS6 by the anti-NACOS6 Ab and COS6 by the anti-COS6 and the anti-COSM Abs in the cdELISAs were about 0.2, 2, ana 2 ng/ml (ppb), respectively. In the cdELISA, the anti-NACOS6 Ab was found to recognize NACOS3-NACOS6, but not N-acetyl-D-glucosamine (GlcNAc), NACOS2, and COSs; the anti-COS6 Ab recognized COS2-COS6 and COSM, but not glucosamine (GlcN) and NACOSs. The recognition pattern of the anti-COSM Ab was almost the same as that of the anti- COS6 Ab, except that the former recognized COS2 and COS3 slightly better than the latter.
Keywords
N-acetylchitooligosaccharides (NACOS); chitooligosaccharides (COS); polyclonal antibody; ELISA; cross-reactivity;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
연도 인용수 순위
1 Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meht-A solid tumor /
[ Tokoro, A.;N. Tatewaki;K. Suzuki;T. Mikami;S. Suzuki;M. Suzuki ] / Chem. Pharm. Bull.   DOI
2 Enzyme-linked immunosorbent assay for detection of chitooligosaccharides /
[ Kim, S. Y.;D. H. Shon;K. H. Lee ] / Biosci. Biotechnol. Biochem.   DOI   ScienceOn
3 Recovery and utilization of chitin and chitosan in food processing waste management /
[ Knorr, D. ] / Food Technology
4 Purification and enzymatic properties of chitosanase from Bacillus sp. No.7-M /
[ Uchida, Y.;M. Izume;A. Ohtakara ] / Bull. Fac. Agr. Sage Univ.
5 Purification and properites of chitosanase from Bacillus circulans MH-K1 /
[ Yabuki, M.;A. Uchiyama;K. Suzuki;A. Ando;T. Fujii ] / J. Gen. Appl. Microbiol.   DOI
6 Gel electrophoresis under denaturing conditions /
[ Bollag, D. M.;M. D. Rozycki;S. J. Edelstein ] / Protein Method
7 Antituor activity of chitosan oligosaccharides produced in ultrafiltration membrane reactor system /
[ Jeon, Y. J.;S. K. Kim ] / J. Microbiol. Biotechnol.
8 Characterization of the smallest chitosan oligomer that is maximally antifungal to Fusrium solani and elicits pisatin formation in Pisum sativum /
[ Kendra, D.;L. Hadwiger ] / Exp. Mycology   DOI
9 Chitosan oligomers from Fusarium solani/pea interactions, chitinase/-glucanase digestion of sporelings and from fungal wall chitin actively inhibit fungal growth and enhance disease resistance /
[ Kendra, D. F.;D. Christian;L. A. Hadwiger ] / Physiol. Mol. Plant Pathol.   DOI
10 Synthetic of a matrix-supported enzyme in non-aqueous conditions /
[ Bartling, G. J.;H. D. Brown;S. K. Chattopadhyay ] / Nature
11 Immunobilization techniques for enzymes /
[ Hearn, M. T. W. ] / Meth. Enzymol.   DOI
12 Applications of chitin and chitosan as fiber and textile chemicals /
[ Hudson, S. M.;A. Domard(ed.);G. A. F.Roberts(ed.);K. M. Varum(ed.) ] / Advances in Chitin Science
13 Detection of Fusarium species by enzyme-linked immunosorbent assay using monoclonal antibody /
[ Kwak, B. Y.;B. J. Kwon;C. H. Kweon;D. H. Shon ] / J. Microbiol. Biotechnol.
14 Detection of Aspergillus, Penicillium, and fusarium species by sandwich enzyme-linked immunosorbent assay using mixed monoclonal antibodies /
[ Kwak, B. Y.;B. J. Kwon;C. H. Kweon;D. H. Shon ] / J. Microbiol. Biotechnol.
15 Aldonate coupling, a simple procedure for the preparation of carbohydrate-protein conjugates for studies of carbohydratebinding proteins /
[ Lonngren, J.;I. J. Goldstein;J. E. Neiderhuber ] / Arch. Biochem. Biophys.   DOI   ScienceOn
16 Effect of D-glucosamine on human natural killer activity in vitro /
[ Matheson, D. S.;B. J. Green;S. J. Friedman ] / J. Biol. Resp. Modif
17 Stimulation of cytokine production in mice using deacetylatd chitin /
[ Nishimura, K.;C. Ishihara;S. Ukei;S. Tokura;I. Azuma ] / Vaccine   DOI   ScienceOn
18 Variation of antifungal activities of chitosans on plant pathogens /
[ Park, R. D.;K. J. Jo;Y. Y. Jo;Y. L. Jin;K. Y. Kim;J. H. Shim;Y. W. Kim ] / J. Microbiol. Biotechnol.
19 The determination of glucosamine and galactosamine /
[ Rondle, C. J. M.;W. T. J. Morgan ] / Biochem. J.   DOI
20 Enhancing effect of N-acetylchitooligosaccharides on the active oxygen-generating and microbicidal activitites of peritoneal exudate cells in mice /
[ Suzuki, K.;A. Tokoro;Y. Okawa;S. Suzuki;M. Suzuki ] / Chem. Pharm. Bull.   DOI
21 Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose /
[ Suzuki, K.;T. Mikami;Y. Okawa;A. Tokoro;S. Suzuki;M. Suzuki ] / Carbohydr. Res.   DOI   ScienceOn