Browse > Article

Quantitative Changes of PR Proteins and Antioxidative Enzymes in Response to Glomus intraradices and Phytophthora capsici in Pepper (Capsicum annuum L.) Plants  

Zheng, Hu-Zhe (Division of Applied Bioscience and Biotechnology, APSRC, College of Agriculture & Life Science, Chonnam National University)
Kim, Yong-Woong (Division of Applied Bioscience and Biotechnology, APSRC, College of Agriculture & Life Science, Chonnam National University)
Lee, Hyun-Jin (Division of Applied Bioscience and Biotechnology, APSRC, College of Agriculture & Life Science, Chonnam National University)
Park, Ro-Dong (Glucosamine Saccharide Materials Laboratory (NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University)
Jung, Woo-Jin (Glucosamine Saccharide Materials Laboratory (NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University)
Kim, Young-Cheol (Division of Applied Plant Science, APSRC, Chonnam National University)
Lee, Sang-Hyun (Division of Applied Plant Science, APSRC, Chonnam National University)
Kim, Tae-Hwan (Division of Animal Science, Institute of Agriculture Science & Technology, Chonnam National University)
Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, APSRC, College of Agriculture & Life Science, Chonnam National University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.3, 2004 , pp. 553-562 More about this Journal
Abstract
To investigate protective activity in pepper plants, which were pre-inoculated with arbuscular mycorrhizal (AM) fungi Glomus intra radices (Gi), against pathogenic strain Phytophthora capsici (Pc), pathogenesis-related (PR) proteins and antioxidant enzymes were examined. The growth of root and shoot was the highest in peppers inoculated with G. intraradices, compared with non-inoculated control plants and those challenged by the pathogen with and without mycorrhizae after nine days of infection. Mycorrhizal colonization rate was reduced by about 10% in pathogen-challenged plants, but disease pressure was reduced. The activities of PR proteins, $\beta$-1- 3-glucanase and chitinase, were increased in Pc-treated plants compared to Gi+Pc-treated plants in leaves, but those in roots were suppressed. Superoxide dismutase activity and $H_2O_2${/TEX> content in Gi+Pc and Pc-treated plants were gradually increased in leaves. However, those in roots continuously increased up to 5 days, and then decreased dramatically. Peroxidase activity in leaves and roots increased after P. capsici infection both in plants inoculated with or without G. intraradices. These results suggest that AM fungi, G. intra radices, potentially act as one of the protective agents against plant pathogens. Changes of PR proteins and antioxidative enzymes in mycorrhizae-inoculated pepper appear to be regulated differently in leaves and roots by pathogen infection.
Keywords
Biological control; arbuscular mycorrhizal fungi; Glomus intra radices; Phytophthora capsici; pathogenesis-related proteins;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
연도 인용수 순위
1 Cell wall peroxidase activity,hydrogen peroxide level and NaCl-inhibited root growth of rice seedings /
[ Lin,C.C.;C.H.Kao ] / Plant Soil   DOI   ScienceOn
2 Absorption of light by chlorophyll solution /
[ Mackinney,G. ] / J. Biol. Chem.
3 Decreased susceptibility to viral disease of β-1-3-glucanase-deficient plants generated by antisense transformation /
[ Beffa,R.S.;R.M.Hofer;M.Thomas;F.Meins ] / Plant Cell   DOI   ScienceOn
4 Localized verus systemic effect of arbuscular mycorrhizal fungi on defense responses to Phytophthora infection in tomato plants /
[ Pozo,M.J.;C.Cordier;E.Dumas Gaudot;S.Gianinazzi;J.M.Barea;C.Azcon Aguilar ] / J. Exp. Bot.   DOI   ScienceOn
5 Pathogenesis-related(PR) proteins do not play a central role in TMV localization in Nicotiana rustica /
[ Dumas,D.;S.Gianinazzi ] / Physiol. Mol. Plant Pathol.   DOI
6 Vesicular arbuscular mycorrhiza in native vegatation system /
[ Read,D.J.;H.K.Koucheki;J.Hodgson ] / New Phytol.   DOI   ScienceOn
7 Induction of systemic resistance in cucumber against several diseases by plant growth-promoting fungi:Lignification and superoxide generation /
[ Koike,N.;M.Hyakumachi;K.Kageyama;S.Tsuyumu;N.Doke ] / Eur. J. Plant Pathol.   DOI   ScienceOn
8 Colonization patterns of root tissues by Phytophthora nicotianae var.parasitica related to reduced disease in mycorrhizal tomato /
[ Cordier,C.;S.Gianinazzi;V.Gianinazzi Pearson ] / Plant Soil   DOI   ScienceOn
9 Cellular and molecular defense-related root responses to invasion by arbuscular mycorrhizal fungi /
[ Gianinazzi Pearson,V.;E.Dumas Gaudot;A.Gollotte;A.Tahiri Alaoui;S.Gianinazzi ] / New Phytol.   DOI   ScienceOn
10 The defense response elicited by the pathogen Rhizoctonia solani is suppressed by colonization of VM-gungus Glomus intraradices /
[ Guenoune,D.;S.Galili;D.A.Phillips;H.Volpin;I.Chet;Y.Okon;Y.kapulnik ] / Plant Science   DOI   ScienceOn
11 Induction of jasmonate biosynthesis in arbuscular mycorrhizal barley roots /
[ House,B.;W.Maier;O.Miersch;R.Kramell;D.Strack ] / Plant Physiol.   DOI   ScienceOn
12 Identification of FM001 as plant growth-promoting substance from Acremonium strictum MJNI culture /
[ Jung,J.H.;D.M.Shin;W.C.Bae;S.K.Hong;J.W.Suh;S.G.Koo;B.C.Jeong ] / J. Microbiol. Biotechnol.   과학기술학회마을
13 Cold-storage of mixed inoculum of Glomus intraradices enhances root colonization,phosphorus status and growth of hot pepper /
[ Kim,K.Y.;Y.S.Cho;B.K.Sohn;R.D.Park;J.H.Shim;S.J.Jung;Y.W.Kim;K.Y.Seong ] / Plant Soil   DOI   ScienceOn
14 Differential production of superoxide dismutase and catalase isozymes during infection of wheat by a Fusarium proliferatum-like isolate /
[ Kwon,S.I.;A.J.Anderson ] / Physiol. Mol. Plant Pathol.   DOI   ScienceOn
15 Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression /
[ Pietrse,C.M.J.;S.C.M.Van Wees;E.Hoffland;J.A.Van Pelt;L.C.Van Loon ] / Plant Cell   DOI   ScienceOn
16 Defense-related transcript accumulation in Phaseolus vulgaris L.colonized by the arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith /
[ Blee,K.A.;A.J.Anderson ] / Plant Physiol.   DOI
17 The induction of gene expression in response to pathogenic microbes /
[ Collinge,D.B.;P.L.Gregersen;H.Thordal Christensen;A.S.Besra(ed.) ] / Mechanisms of Plant Growth and Improved Productivity,Modern Approaches and Perspectives
18 Subcellular localization of chitinase and of ifs potential substrates in tomato root tissues infected by Fusarium oxysporium f.sp. radicis-lycopersici /
[ Benhamou,N.;M.H.A.J.Joosten;P.J.G.M.De Wit ] / Plant Physiol.   DOI   ScienceOn
19 Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacetria /
[ Wei,G.;J.W.Kloepper;S.Tuzun ] / Phytopathology   DOI
20 Induction and accumulation of PR protein activities during early stages of root colonization by the mycoparasite Trichoderma harzianum strain T-203 /
[ Yedidia,I.;N.Benhamou;Y.Kapulnik;I.Chet ] / Plant Physiol. Biochem.   DOI   ScienceOn
21 Differential accumulation of β-1-3-glucanase and chitinase isoforms in pepper stems infected by compatible and incompatible isolated of Phytophthora capsici /
[ Kim,Y.J.;B.K.Hwang ] / Physiol. Mol. Plant Pathol.   DOI   ScienceOn
22 Isolation of a basic 34 kilodalton β-1-3-glucanase with inhibitory activity against Phytophthora capsici from pepper stems /
[ Kim,Y.J.;B.K.Hwang ] / Physiol. Mol. Plant Pathol.   DOI   ScienceOn
23 Hydrogen peroxide plays a critical role in the defense response of tomato to Cladosporium fulvum /
[ Borden,S.;V.J.Higgins ] / Physiol. Mol. Plant Pathol.   DOI   ScienceOn
24 A plant growth promoting Pseudomonas fluorescens GL20:Mechanism for disease suppression,outer membrane receptors for ferric siderophore, and genetic improvement for increased biocontrol efficacy /
[ Lim,H.S.;J.M.Lee;S.D.Kim ] / J. Microbiol. Biotechnol.   과학기술학회마을
25 Induced systemic resistance in radish is not associated with accumulation of pathogenesis-related proteins /
[ Hoffland,E.;C.M.J.Pieterse;L.Bik;J.A.Van Pelt ] / Physiol. Mol. Plant Pathol.   DOI   ScienceOn
26 Cell defense response associated with localized and systemic resistance to Phytophthora parasitica induced in tomato by an arbuscular mycorrhizal fungus /
[ Cordier,C.;M.J.Pozo;J.M.Barea;S.Gianinazzi;V.Gianinazzi Pearson ] / Mol. Plant Microbe Interact   DOI   ScienceOn
27 Induction of systemic resistance of tobacco to necrosis virus by the root-colonizing Pseudomonas fluorescene strain CHA0:Influence of the gacA gene and of pyoverdine production /
[ Maurhofer,M.;C.Hase;P.Meuwly;J.P.Metraux;G.Defago ] / Phytopathology   DOI   ScienceOn
28 The role of active oxygen species in plant signal transduction /
[ Breusegem,F.V.;E.Vranova;J.F.Dat;D.Inze ] / Plant Sci.   DOI   ScienceOn
29 Oxygen toxicity and superoxide dismutase as an antioxidant in physiological stress /
[ Monk,L.S.;K.V.Fagerstedt;M.M.Crawford ] / Physiol. Plant   DOI
30 Interaction between the soilborne root pathogen Phytophthora nicotianae var.parasitica and the arbuscular mycorrhizal fungus Glomus mosseae in tomato plants /
[ Trotta,A.;G.C.Varese;E.Gnavi;A.Fusconi;S.Sampo;G.Berta ] / Plant Soil   DOI   ScienceOn
31 Chitosanase and chitinase activities in tomato roots during interactions with arbuscular mycorrhizal fungi or Phytophthora parasitica /
[ Pozo,M.J.;C.Azcon Aguilar;E.Dumas Gaudot;J.M.Barea ] / J. Exp. Bot.   DOI   ScienceOn
32 Carbohydrate accumulation in relation to heat stress tolerance in two creeping bentgrass cultivars /
[ Liu,X.Z.;B.R.Huang ] / J. Am. Soc. Hort. Sci.
33 Biocontrol of selected soilborne diseases of tomato and pepper plants /
[ Mao,W.;J.A.Lewis;R.D.Lumsden;K.P.Hebbar ] / Crop Prot.   DOI   ScienceOn
34 β-1-3-glucanase activities in tomato roots inoculated with arbuscular mycorrhizal fungi and/or Phytophthora parasitica and their possible involvement in bioprotection /
[ Pozo,M.J.;C.Azcon-Aguilar;E.Dumas Gaudot;J.M.Barea ] / Plant Sci.   DOI   ScienceOn
35 Involvement of antiozidants and lipid peroxidation in the adaptation of two cool-season grasses to licalized drought stress /
[ Fu.J.M.;B.R.Huang ] / Environ. Exp. Bot.   DOI   ScienceOn
36 Suppression of endochitinase, β-1-3-endoglucanse,and chalcone isomerase expression in bean vesicular-arbuscular mycorrhizal roots under different soil phosphate conditions /
[ Lambias,M.R.;M.C.Mehdy ] / Mol. Plant Microbe Interact   DOI
37 Do pathogenesis-related(PR) proteins play a role in bioprotection of mycorrhizal tomato roots towards Phytophthora prasitica? /
[ Dassi,B.;E.Dumas Gaudot;S.Gianinazzi ] / Physiol. Mol. Plant Pathol   DOI   ScienceOn
38 A new method for observing the morphology of vesicular arbuscular mucorrhizae /
[ Brundrett,M.C.;Y.Piche;R.L.Peterson ] / Can. J. Bot.   DOI
39 /
[ Agrios,G.N. ] / Plant Pathology,4th Ed.
40 Comparison of systemic resistance induced by avirulent and nonpathogenic Pseudomonas species /
[ Hoffland,E.;J.Hakulinen;J.A.Van Pelt ] / Phytopathology   DOI   ScienceOn
41 Arbuscular mycorrhizas and biological control of soil-borne plant pathogens-an overview of the mechanisms involved /
[ Azcon Aguilar,C.;J.M.Barea ] / Mycorrhiza   DOI   ScienceOn