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Dithiothreitol Attenuates the Pathogenic Interaction Between Pseudomonas aeruginosa and Drosophila melanogaster  

Lee, Ji-Sun (Department of Life Science, Sogang University)
Kim, Seol-Hee (Department of Life Science, Sogang University)
Cho, You-Hee (Department of Life Science, Sogang University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.2, 2004 , pp. 367-372 More about this Journal
Abstract
Infection of Drosophila melanogaster adults with Pseudomonas aeruginosa (PA14) can kill the flies within 48h. We found that the virulence of PA14 was significantly attenuated when treated with a reducing agent, dithiothreitol (DTT), prior to infection. Infection with DTT-treated PA14 elevated Metchnikowin expression at 22 h post-infection and the virulence of DTT-treated PA14 was not attenuated in Dif and Relish mutants. These results suggest that DTT pre-treatment of PA14 can aggravate certain virulence factors that may be required to paralyze fly immune responses, triggering Metchnikowin expression via Dif and Relish activations.
Keywords
Redox; Pseudomonas; Drosophila; virulence; dithiothreitol; immune response;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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