One-step Purification of Poly-His Tagged Penicillin G Acylase Expressed in E. coli
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Kim, Jin-Hee
(Department of Biologicla Engineering, ERC for the Advanced Bioseparation Technology, Inha University)
Kang, Hye-Jin (Department of Biologicla Engineering, ERC for the Advanced Bioseparation Technology, Inha University) Kim, Eung-Soo (Department of Biologicla Engineering, ERC for the Advanced Bioseparation Technology, Inha University) Kim, Jeong-Ho (Department of Biology, Inha University) Koo, Yoon-Mo (Department of Biologicla Engineering, ERC for the Advanced Bioseparation Technology, Inha University) |
1 |
The effect of polyol compounds on the thermostability of penicillin G acylase from a mutant of Escherichia coli ATCC 11105
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2 |
Identification of catalytically essential amino acid residues of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105
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3 |
Cloning and expression of the penicillin acylase gene (pac) from E. coli ATCC 11105
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4 |
Clarification of penicillin G acylase reaction mechanism
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5 |
Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945
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6 |
Enhanced production of penicillin G acylase from a recombinant Escherichia coli
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DOI ScienceOn |
7 |
Isolation of penicillin G acylase from Escherichia coli ATCC 11105 by physical and chemical treatments
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DOI ScienceOn |
8 |
Penicillin acylase purification with the aid of pseudo-affinity chromatography
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DOI ScienceOn |
9 |
Three-step chromatographic purification procedure for the production of a His-tag recombinant kinesin overexpressed in E. coli
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10 |
Influence of polyhydric compounds on the pH stability of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105
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11 |
Evaluation of immobilized metal affinity chromatography for purification of penicillin acylase
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12 |
Improved penicillin amidase production using a genetically engineered mutant of Escherichia coli ATCC 11105
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13 |
Production of immobilized penicillin acylase using aqueous polymer systems for enzyme purification and in situ immobilization
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DOI ScienceOn |
14 |
Efficient two-step chromatographic purification of penicillin acylase from clarified Escherichia coli ultrasonic homogenate
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DOI |
15 |
Rapid and simple method to prepare functional Pfu DNA polymerase expression in Escherichia coli periplasm
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과학기술학회마을 |
16 |
Penicillin G acylase from E. coli: Unique gene-protein relation
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DOI ScienceOn |
17 |
The isolation and kinetics of penicillin amidase from Escherichia coli
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DOI ScienceOn |
18 |
Reconstitution in vivo of penicillin G acylase activity from separately expressed subunits
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DOI ScienceOn |
19 |
Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports
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20 |
Purification and kinetics of penicillin G acylase from a mutant strain of Escherichia coli ATCC 11105
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DOI |
21 |
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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DOI ScienceOn |
22 |
Characterization of glutaryl 7-ACA acylase from pseudomonas diminuta KAC-1
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과학기술학회마을 |
23 |
Complete nucleotide sequence of the penicillin G acylase gene and the flanking regions and its expression in Escherichia coli
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24 |
Colorimetric protein assay techniques
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25 |
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