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Expression of E. coli Phosphofructokinase Gene in an Autotrophic Bacterium Acidithiobacillus thiooxidans  

Tian, Ke-Li (State Key Laboratory of Microbial Technology, Shandong University)
Lin, Jian-Qun (State Key Laboratory of Microbial Technology, Shandong University)
Liu, Xiang-Mei (State Key Laboratory of Microbial Technology, Shandong University)
Liu, Ying (State Key Laboratory of Microbial Technology, Shandong University)
Zhang, Chang-Kai (State Key Laboratory of Microbial Technology, Shandong University)
Yan, Wang-Ming (State Key Laboratory of Microbial Technology, Shandong University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.1, 2004 , pp. 56-61 More about this Journal
Abstract
A plasmid pSDK-l containing the Escherichia coli phosphofructokinase-l gene (pfkA) was constructed, and transferred into extremely acidophilic Acidithiobacillus thiooxidans Tt-7 by conjugation with the aid of plasmid RP4 at a frequency of $10^{-5}$ per recipient. This plasmid was stable in A. thiooxidans. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium, but the enzyme activity (21.6 U/g protein) was lower than that in E. coli (K12: 85.9 Dig protein; DF1010 carrying plasmid pSDK-l: 96.6 U/g protein). In the presence of glucose, the Tt-7 transconjugants consumed glucose, leading to a better growth yield.
Keywords
Acidithiobacillus thiooxidans; phosphofructokinase; conjugation;
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1 Barros, M. E., D. E. Rawlings, and D. K. Woods. 1986. Purification and regulation of T. ferrooxidans glutamine synthetase cloned in Escherichia coli. J. Gen. Microbiol. 132: 1989-1995
2 Berger, D. K., D. K. Woods, and D. E. Rawlings. 1990. Complementation of Escherichia coli $\sigma$$^54$ (ntrA)-dependent formatehydrogen-lyase activity by a cloned T. ferrooxidans ntrA gene. J. Bacteriol. 172: 4399-4406   DOI
3 Darbre, A. 1986. Analytical methods, pp. 227-335. In A. Darbre (ed.), Practical Protein Chemistry. A Handbook. John Wiley and Sons, New York, U.S.A
4 Guiliani, N., A. Bengrine, F. Borne, Chippaux, and V. Bonnefoy. 1997. Alanyl-tRNA synthetase gene of the extreme acidophilic chemolithoautotrophic Thiobacillus ferrooxidans is highly homologous to alaS genes from all living kingdoms but cannot be transcribed from its promoter in Escherichia coli. Microbiology 143: 2179-2187
5 Blattner, F. R., G. III. Plunkett, C. A. Bloch, et al. 1997. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1474
6 Jin, S. M., W. M. Yan, and Z. N. Wang. 1993. Development of a conjugative transfer system for Thiobacillus thiooxidans. Chinese Journal of Biotechnology 9: 87-89
7 Datta, N., R. W. Hedges, E. J. Shaw, R. B. Sykes, and M. H. Richmond. 1971. Properties of an R factor from Pseudomonas aeruginosa. J. Bacteriol. 108: 1244-1249
8 Hellinga, H. W. and P. R. Evans. 1985. Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase. Eur. J. Biochem. 149: 363-373
9 Birnboim, H. C. and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7: 1513-1523
10 Shao, Q., W. M. Yan, Z. Y. Liu, and D. Y. Yao. 1997. Construction of arsenic resistance gene containing plasmid pSGR941 and its expression in the obligately autotrophic acidophilic Thiobacillus thiooxidans. Journal of Shandong University 32: 348-352
11 Jin, S. M., W. M. Yan, and Z. N. Wang. 1992. Transfer of IncP plasmids to extremely acidophilic Thiobacillus thiooxidans. Appl. Environ. Microbiol. 58: 429-430
12 Inoue, H., T. Tamura, N. Ehara, A. Nishito, Y. Nakayama, M. Maekawa, K. Imada, H. Tanaka, and K. Inagaki. 2002. Biochemical and molecular characterization of the NAD+- dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans. FEMS Microbiol. Lett. 214: 127-132
13 Davison, J., M. Heusterspreute, N. Chevalier, V. Ha-Thi, and F. Brunel. 1987. Vectors with restriction site banks V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites. Gene 51: 275-280   DOI   ScienceOn
14 Kotlarz, D. and H. Buc. 1982. Phosphofructokinases from Escherichia coli. Methods Enzymol. 90: 60-70   DOI
15 Kusano, T., K. Sugawara, C. Inoue, and N. Suzuki. 1991. Molecular cloning and expression of Thiobacillus ferrooxidans chromosomal ribulose bisphosphate carboxylase genes in Escherichia coli. Curr. Microbiol. 22: 35-41
16 Matin, A. 1978. Organic nutrition of chemolithotrophic bacteria. Annu. Rev. Microbiol. 32: 433-468
17 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227: 680-685   DOI   PUBMED   ScienceOn
18 Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467   DOI   ScienceOn
19 Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY, U.S.A
20 Hoover, T. R., E. Santero, S. Porter, and S. Kustu. 1990. The integration host factor stimulates interaction of RNA polymerase with NifA, the transcriptional activator for nitrogen fixation operons. Cell 63: 11-22