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Isolation and Analysis of the argG Gene Encoding Argininosuccinate Synthetase from Corynebacterium glutamicum  

Ko, Soon-Young (Department of Biological Science, Sookmyung Women's University)
Kim, Sei-Hyun (Department of Biological Science, Sookmyung Women's University)
Lee, Heung-Shick (Department of Biotechnology, Korea University)
Lee, Myeong-Sok (Department of Biological Science, Sookmyung Women's University)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.6, 2003 , pp. 949-954 More about this Journal
Abstract
The argG gene of Corynebacterium glutamicum encoding argininosuccinate synthetase (EC6345) was cloned and sequenced. The gene was cloned by heterologous complementation of an Escherichia coli arginine auxotrophic mutant (argG/sup -/). The cloned DNA fragment also complements E. coli argD, argF, and argH mutants, suggesting a clustered organization of the genes in the chromosome. The coding region of the argG gene is 1,206 nucleotides long with a deduced molecular weight of about 44 kDa, comparable with the predicted size of the expressed protein on the SDS-PAGE. Computer analysis revealed that the amino acid sequence of the argG gene product had a high similarity to that of Mycobacterium tuberculosis and Streptomyces clavuligerus. Two conserved sequence motifs within the ArgG appear to be ATP-binding sites which correspond to 2 of the 3 conserved regions found in sequences of all known argininosuccinate synthetases.
Keywords
Corynebacterium glutamicum; argG; argininosuccinate synthetase; heterologous complementation;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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1 molecular organization and regulation of the biosynthetic pathway for aspartate-derived amino acids in corynebacterium gluatamicum /
[ Jetten,M.S.;M.E.Gubler;M.M.McCormick;G.E.Coln;M.T.Follettie;A.J.SInskey;R.H.Baltz(ed.);G.D.Hegeman(ed.);P.L.Skatrud(ed.) ] / Industral Microoganisms: Basis and Applied Molecular Genetics
2 Dynamic respiratory measurements of Corynebacterium glutamicum using membrane mass spectrometry /
[ Wittmann,C.;T.Yang;I.Kochems;E.Heinzle ] / J. Microbiol. Biotehcnol.   과학기술학회마을
3 Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence /
[ Cole,S.T.;R.Brosch;J.Parkhill;T.Garnier;C.Churche;D.Harris;S.V.Gordon;K.Eiglmeier;S.Gas;C.E.Barry;F.Tekaia;K.Badcock;D.Basham;D.Brown;T.Chillingworth;R.Connor;R.Davies;K.Devlin;T.Feltwell;S.Gentles;N.Hamlin;S.Holoyd;T.Hornsby;K.Jegels;B.G.Barrell ] / Nature   DOI   ScienceOn
4 Moleclular basic for modulated regulation of gene expression in the arginine regulation of gene expression in the arginine regulon of Escherichia coli /
[ Cunin,R.;T.Eckhardt;J.Piette;A.Boyen;A.Pierard;N.Glansdorff ] / Nucleic Acides Res.   DOI   ScienceOn
5 Genetic engineering of coryneform bacteria /
[ Batt,C.A.;M.T.Follettie;H.K.Shin;P.Yeh;A.J.Sinskey ] / Trends Biotechnol.   DOI   ScienceOn
6 Molecular coloing and analysis of the argC gene from Corynebacterium glutamicum /
[ Chun,J.Y.;E.J.Lee;H.S.Lee;C.J.Cheon;K.H.Min;M.Y.Lee ] / Mol. Biol. Intl.
7 Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus /
[ Rodriguez-Garcia,A.;de la Fuente;R.Perez-Pedondo;J.F.Martin;P.Liras ] / J. Mol. Microbiob. Biotechnol.
8 The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa /
[ Haas,D.;B.W.Holloway;A.Schambock;T.Leisinger ] / Mol. Gen. Genet.   DOI   ScienceOn
9 Molecular characterization of the levansucrase gene from Pseudomonas aurantiaca S-4380 and its expression in Escherichia coli /
[ Jang,E.K.;K.H.Jang;I.Koh;I.H.Kim;S.H.Kim;S.A.Kang;C.H.Kim;S.D.Ha;S.K.Rhee ] / J. Microbiol. Biotechnol.   과학기술학회마을
10 Biosynthesis and metabolism of arginine in bacteria /
[ Gunin,R.;N.Glasdorff;A.Piecard;V.S.Stalon ] / Microb. Rev.
11 Cloning of the argF gene encoding the ornithine carbamoyltranferase from Corynebacterium glutamicum /
[ Chun,J.Y.;M.S.Lee ] / Mol. Cell.   과학기술학회마을
12 The argG gene of Streptomyces clavuligerus has low homology to unstable argG from other actiomycetes: Effect of amplification on clavulanic acid biosynthesis /
[ Rodriguez-Gracia,A.;J.F.Martin;P.Liras ] / Gene   DOI
13 Arginine biosynthesis in gonococci isolated from patients /
[ Catlin,B.W.;E.H.Nash;G.F.Brooks(ed.);E.C.Gotschlich(ed.);K.K.Holmes(ed.);W.D.Sawyer(ed.);F.F.Young(ed.) ] / Immunology of Neisseria gonorrhoeae
14 Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis /
[ Mountain,A.;N.H.Mann;R.N.Munton;S.Baumberg ] / Mol. Gen. Genet.   DOI   ScienceOn
15 Cloning and endonuclease restriction analysis of the argF and of the control region of the argECBH bipolar operon n Escherichia coli /
[ Crabeel,M.;D.Charlier;R.Cunin;N.Glansdorff ] / Gene   DOI   ScienceOn
16 Genetic engineering of Gram positive bacteria /
[ Wohlleben,W.;G.Muth;J.Kalinowski;H.J.Rehm(ed.);G.Reed(ed.);A.Puhler(ed.);P.Stadler(ed.) ] / Biotechnology: Vol.2, Fundamentals and Genetic Engineering
17 The genus Corynebacterium-nonmedical /
[ Libel,W. ] / The Prokaryotes
18 Purification and characterization of extracellular and intracellular glutamine synthetases from Mycobacterium bovis BCG /
[ Suh,C.I;J.M.Lim;H.C.Sung ] / J. Microbiol. Biotechnol.   과학기술학회마을
19 Molecular characterization of AceB, a gene encoding malate synthase in Corynebacterium glutamicum /
[ Lee,H.S.;A.J.Sinskey ] / J. Microbiol. Biotechnol.   과학기술학회마을
20 Purification and characterization of 2,4-dichlorophenol oxidizing peroxidase from Streptomyces sp. AD001 /
[ Jeon,J.H.;Y.J.Han;T.G.Kang;E.S.Kim;S.K.Hong;B.C.Jeong ] / J. Microbiol. biotechnol.   과학기술학회마을
21 Regulation of arginine biosynthesis in prokaryotes /
[ Cunin,R.;K.M.Herrmann(ed.);R.L.Somerville(ed.) ] / Amino Acids: Biosynthesis and Genetic Regulation
22 Identifiation of a sequence containing methylated cytidine in Corynebacterium glutamicum and Brevibacterium flavum using bisulfite DNA derivatization and sequencing /
[ Jang,K.H.;P.J.Chambers;M.L.Britz ] / J. Microbiol. Biotechnol.   과학기술학회마을
23 Cloning and organization of seven arginine biosynthesis genes from Neisseria gonorrhoeae /
[ Picard,F.J.;J.R.Dillon ] / J. Bacteriol.   DOI
24 Distanity related sequences in the a- and b-subunits of ATP synthase, myosin kinases and other ATP-binding enzymes and a common nucleotide binding fold /
[ Walker,J.E.;M.Saraste;M.J.Runswick;N.J.Gay ] / EMBO J.
25 /
[ Sambrook,J.;E.F.Fritsch;T.Maniatis ] / Molecular Cloning: A Laboratory Manual
26 Primary structure, partial purification and regulation of key enzyme of the acetyl cycle of arginine biosynthesis in Bacillus stearothermophilus: Dual function of ornithe acetyltransferase /
[ Sakanyan,V.;D.Charlier;C.Legrain;A.Kochikyan;I.Mett;A.Pierard;N.Glansodorff ] / J. Gen. Microbiol.
27 Acetylornithinase of Escherichia coli: Partial purification and some properties /
[ Vogel,H.J.;D.M.Bonner ] / J. Biol. Chem.
28 Cloning and expression of the genes Corynebacterium flavum ATCC14067 complementing the argA and argE mutations in Escherichia coli cells /
[ Kharitonov,A.A.;A.P.Tarsaov ] / Molek. Genet. Mikrobiol. Virusol.
29 Cloning of the histidine biosynthetic genes of Corynebacterium glutamicum: organization and sequencing analysis of the hisA, and hisF gene cluster /
[ Jung,S.I.;M.S.Han;J.H.Kwon;C.I.Cheon;K.H.Min;M.S.Lee ] / Biochem. Biophys. Res. Commun.   DOI   ScienceOn
30 Pathway-specific pattern of control of arginine biosynthesis in bacteria /
[ Udaka,S. ] / J. Bacteriol.
31 Sequences of the genes encoding argininosuccinate synthetase in Escherichia coli and Saccharomyces cerevisae: Comparison with methanogenic archaebacteria and mammals /
[ Van.Viet,F.;M.Crabeel;A.Boyen;C.Tricot;V.Stalon;P.Falmagen;Y.Nakamura;S.Baumberg;N.Glansdorff ] / Gene   DOI   ScienceOn
32 Utilization of lacZ to isolate regulatory genes from Corynebacterium glutamicum /
[ Kim,H.J.;J.S.Park;H.S.Lee;Y.Kim ] / J. Microbiol. Biotechnol.   과학기술학회마을
33 Genes and enzymes of the acetyl cycle of arginine biosynthesis in corynebacterium glutamicum: Enzyme evolution in the early steps of the arginine pathway /
[ Sakanyan,V.;P.Petrosyan;M.Lecocq;A.Boyaen;C.Legria;M.Demarez;Jean-Noel Hallet;N.Glansdorff ] / Microbiology   DOI   ScienceOn
34 Gene sequence encoding early enzyme of arginine synthesis within a cluster in Bacillus subtilis, as revealed by cloning in Escherichia coli /
[ Monutain,A.;J.McChesney;M.C.M.Smith;S.Baumberg ] / J. Bacteriol.   DOI
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36 Feedback regulation of arginine biosynthesis in bule-green algae and photosynthetic bacteria /
[ Hoard,D.S.;S.L.Hoare ] / J. Bacteriol.
37 Cloning and sequencing of the amylase gene from Paenibacillus sp. and its expression is Saccharomyces cerevisiae /
[ Jeong,T.H.;H.O.Kim;J.N.Park;H.J.Lee;D.J.Shin;H.B.Lee;S.B.Chun;S.Bai ] / J. Microbiol. Biotechnol.   과학기술학회마을