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Effect of Lactobacillus fermentum MG590 on Alcohol Metabolism and Liver Function in Rats  

Kim, Ji-Hyun (Department of Biotechnology and Bioengineering, Pukyong National University)
Kim, Hyun-Jin (Department of Biotechnology and Bioengineering, Pukyong National University)
Son, Jeong-Hwa (Department of Biotechnology and Bioengineering, Pukyong National University)
Chun, Ho-Nam (Mail Dairy Industry Co., Ltd.)
Yang, Jin-Oh (Mail Dairy Industry Co., Ltd.)
Choi, Sung-Jin (Mail Dairy Industry Co., Ltd.)
Paek, Nam-Soo (Mediogen Corp.)
Choi, Gyoung-Hoon (Mediogen Corp.)
Kim, Sung-Koo (Department of Biotechnology and Bioengineering, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.6, 2003 , pp. 919-925 More about this Journal
Abstract
Alcohol consumption has numerous health consequences for the human body. For example, heavy drinking on a daily basis causes liver diseases, and certain products such as acetaldehyde produced from alcohol metabolism are more toxic than alcohol itself. Accordingly, the current study evaluated the role of Lactobacillus fermentum MG590 to enhance the removal of the toxic effect of alcohol in alcohol metabolism. The maximum activities of the alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) by L. fermentum MG590 were observed after 6 h of culture. The production of ADH and ALDH by L. fermentum MG590 was also confirmed by SDS-PAGE. Six hours after the addition of alcohol to a culture broth of L. fermentum MG590, the alcohol concentration decreased from 7.5 to 2.7％. From an in vitro evaluation based on hepatocytes, the viability of hepatocytes in a medium containing alcohol and the cytosol of L. fermentum MG590 was higher than that in a medium containing only alcohol. From an in vivo test using SD rats fed a 22％ alcoholic drink, the blood alcohol concentration (BAC), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) in the rats fed a medium containing L. fermentum MG590 were lower than those in the rats fed a medium containing only the alcohol drink. These results demonstrate that the ADH and ALDH produced by L. fermentum MG590 play an important role in detoxicating alcohol in vivo. Therefore, a fermentation broth of L. fermentum MG590 could be used as an effective alcohol detoxification drink.
Keywords
L. fermentum MG590; ADH; ALDH; liver; BAC; GOT; GPT;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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