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Involvement of Growth-Promoting Rhizobacterium Paenibacillus polymyxa in Root Rot of Stored Korean Ginseng  

Jeon, Yong-Ho (School of Agricultural Biotechnology & Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Chang, Sung-Pae (School of Agricultural Biotechnology & Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Hwang, In-Gyu (School of Agricultural Biotechnology & Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Kim, Young-Ho (School of Agricultural Biotechnology & Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.6, 2003 , pp. 881-891 More about this Journal
Abstract
Paenibacillus polymyxa is a plant growth-promoting rhizobacterium (PGPR) which can be used for biological control of plant diseases. Several bacterial strains were isolated from rotten roots of Korean ginseng (Panax ginseng C. A. Meyer) that were in storage. These strains were identified as P. polymyxa, based on a RAPD analysis using a P. polymyxa-specific primer, cultural and physiological characteristics, an analysis utilizing the Biolog system, gas chromatography of fatty acid methyl esters (GC-FAME), and the 16S rDNA sequence analysis. These strains were found to cause the rot in stored ginseng roots. Twenty-six P. polymyxa strains, including twenty GBR strains, were phylogenetically classified into two groups according to the ERIC and BOX-PCR analyses and 16S rDNA sequencing, and the resulting groupings systematized to the degrees of virulence of each strain in causing root rot. In particular, highly virulent GBR strains clustered together, and this group may be considered as subspecies or biovar. The virulence of the strains seemed to be related to their starch hydrolysis enzyme activity, but not their cellulase or hemicellulase activity, since strains with reduced or no starch-hydrolytic activity showed little or no virulence. Artificial inoculation of the highly virulent strain GBR-1 onto the root surfaces of Korean ginseng resulted in small brown lesions which were sunken and confined to the outer portion of the root. Ginseng root discs inoculated in vitro or two-year-old roots grown in soil drenched with the inoculum developed significant rot only when the inoculum density was $10^{6}-10^{7}$ or more colony-forming units (CFU) per ml. These results suggest that P. polymyxa might induce ginseng root rot if their population levels are high. Based on these results, it is recommended that the concentration of P. polymyxa should be monitored, when it is used as a biocontrol agent of ginseng, especially in the treatment of stored roots.
Keywords
Ginseng; biolog; gas chromatography of fatty acid methyl esters; Paenibacillus polymyxa; 16S rDNA; PCR;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 19  (Related Records In Web of Science)
연도 인용수 순위
1 Bacterial seedling blight of tomato caused by Bacillus polymyxa /
[ Caruso,F.I.;MG.Zuck;A.E.Bessette ] / Plant Dis.   DOI
2 Molecular identification of rRNA group 3 Bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test; proposal for the creation of a new genus Paceibacillus /
[ Ash,C;F.G.Priest;M.D.Collins ] / Antonie van Leeuwenhoek   DOI   ScienceOn
3 White bloch incited in wheat by Bacillus megaterium pv. cerealis /
[ Hosford,R.M.Jr. ] / Phytopathology   DOI
4 Biocontrol of Leptosphaeria maculans and other pathogens of canola with Psenibacillus polymyxa PKBq /
[ Kharbanda,P.D.;J.Yang;P.Beatty;S.Jensen;J.P.Tewari ] / Proc. 10th International Rapseed Congress
5 Endospore forming gram-positive rod and cocci /
[ Sneath,P.H.A.;Krieg,J.R.ed.);J.G.Holt(ed.) ] / Bergey's manual of Systematic Bacteriology
6 A selection strategy for microbial antagonists to control post-harvest diseases of fruits and vegetables /
[ Wilson,C.L.;M.E.Wisniewski;S.Droby;E.Chalutz ] / Sci. Hortic. (Amsterdam)   DOI   ScienceOn
7 Biological control of fungal diseases /
[ Larkin,R.P.;D.P.Roberts;F.N.Martin ] / fungicidal Activity: Chemicaland Biological Approaches to Plant Protection
8 Mechanisms of action and dose-response relationships governing biological control of Fusarium wilt of tomato by nonpathogenic Fusarium spp. /
[ Larkin,R.P.;D.R.Fravel ] / Phytopathology   DOI   ScienceOn
9 Microbiological evaluation of vegetable sprouts and seeds /
[ Prokopowich,D.;G.Blank ] / J. Food Port.   DOI
10 β-Amylase system capable of hydrolyzing raw starch granules form Bacillus polymyxa No. 26 and bacerial identification /
[ Shon,C.B.;M.H.Kim;J.S.Bae;C.H.Kim ] / J. Microbiol. Biotechnol.   과학기술학회마을
11 biological control of Phytophthora blight of red pepper by antagonistic Bacillus polymyxa 'AC-1' /
[ Kiml,Y.K. ] / Soul Nat'l Univ. Ph. D. Thesis
12 Effect of plant growth promoting Bacillus strains on pine and spruce seedling growth and mycorrhizal infection /
[ Shisido,M.;H.B.Massicotte;C.P.Chanway ] / Ann. Bot.   DOI   ScienceOn
13 High populations of Bacillus subtilis associated with phosphorus toxicity in soybeans /
[ Dunleavy,J.;J.F.Funkel;J.J.Hanaway ] / Phytopathology
14 Ginseng growths in abolished ginseng fields and factors affecting the ginseng growth /
[ Kim,Y.H.;J.H.Lee;S.H.Ohh;Y.H.Yu;H.Lee ] / Korean J. Ginseng Sci.   과학기술학회마을
15 POst-harvest biological control of blue-mold on apples /
[ Janisiewicz,W.J. ] / Phytopathology   DOI
16 Assessing genetic diversity of microbes using repetitive-sequence-based PRC (rep-PCR) /
[ Louws,F.J.;M.Schneider;F. J. de Bruijn;Toranzos,G.(ed.) ] / Nucleic Acid Amplification Methods for the Analysis of Environmental Samples
17 Inhibitory activity of Paenibacillus polymyxa SCE2 against human pathogenic micro-organisms /
[ Seldin,L.;F. S. de Azevedo;D.S.Alviano;C.S.Alviano;M. C. de Freire Bastos ] / Lett. Appl. Microbiol.   DOI   ScienceOn
18 /
[ Swoffrd,D.L. ] / PAUP: Phylogenetic Analysis Using Parsimony and Other Mothod
19 Making greater use of introduced microorganisms for biological control of plant pathogens /
[ Cook,R.J. ] / Annu. Rev. Phytopathol.   DOI   ScienceOn
20 Occurrence of Bacillus subtilis in peanut kernels /
[ Petit,R.E.;R.A.Taber;B.G.Foster ] / Phytopathology
21 Bacterial evolution /
[ Woese,C.R. ] / Microbiol. Rev.
22 A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology /
[ Stackebrandt,E.;B.M.Goebel ] / Int. J. Syst.Bacteriol.   DOI   ScienceOn
23 Biocontrol agents of plant pathogens: Their use and practical constraints /
[ Upadhyay,R.S.;B.Rai ] / Biocontrol of Plant Diseases
24 Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR) /
[ Versalovic,J.;M.Schneider;F. J. de Bruijn;J.r.Lupski ] / Method. Cell. Mol. Biol.
25 Antibiosis plays a role in the context of direct interaction during antagonism of Pasnibacillus polymyxa towards Fusarium oxysporum /
[ Dijksterhuis,J.;M.Sanders;L.G.Gorris;E.J.Smid ] / J. Appl. Microbiol.   DOI   ScienceOn
26 Advances in plant health management in the 20th century /
[ Cook,R.J. ] / Annu. Rev. Phytopathol.   DOI   ScienceOn
27 Characterization of a hydrophobic amylase inhibitor from corn (Zea mays) seed with activity against amylase from Fusarium verticillioides /
[ Figueira,E.L.Z.;E.Y.Hirooka;E.Mendiola-Olaya;A.Blanco-Labra ] / Phytopathology   DOI   ScienceOn
28 Easy cloning of mini-Tn10 insertions from the Bacillus subtilis chromosome /
[ Steinmetz,M.;R.Richter ] / J. Bacteriol.   DOI
29 In vitro chitinase and antifungal activity of a soil, rhizosphere and rhizoplane population of Bacillus polymyxa /
[ Mavingui,P.;T.Heulin ] / Soil. Biol. Biochem.   DOI   ScienceOn
30 Inhibition of Bacillus subtilis by Amo-1618 /
[ Dunleavy,J.;J.F.Kunkel ] / Phytopathology
31 Bacillus /
[ Leary,J.V.;W.W.C.Chun ] / Laboratory Guide for Identification of Plant Pathogenic Bacteria(Second edition)
32 Biocontrol of soil-borne plant pathogens /
[ Handelsman,J.;K.Stabb ] / Plant Cell   DOI   ScienceOn
33 Effect of chitinase-producing Paenibacillus illinoisensis KJA-424 on egg hatching of root-knot nematode (Meloidogyne incognita) /
[ Jung,W.J.;S.J.Jung;K.N.An;Y.L.Jin;R.D.Park;K.Y.Kim;B.K.Shon;T.H.Kim ] / J. Microbiol. Biotechnol.   과학기술학회마을
34 Destruction of various tissues in culture by certain bacteria /
[ Volcani,Z.;A.J.Riker;A.C.Hilderbrandt ] / Phytopathology
35 Spore-forming bacteria causing soft rot of potato and retting of flax /
[ Allen,L.A. ] / Nature   DOI
36 Levan-producing Bacillus subtilis Bs 62 and its phylogeny base on its 16S rDNA sequence /
[ Choi,S.H.;S.Chang;W.Y.Chio ] / J. Microbiol. Biotechnol.   과학기술학회마을
37 Production and commericalization of biocontrol products /
[ Fracvel,D.R.;D.J.Rhodes;R.P.Larkin;Albajes,R.(ed.);M.L.Gullino(ed.);J. C. van Lenternen;Y.Elad(ed.) ] / Integrated Pest and Disease Management in Greenhous Crops
38 /
[ Agrios,G.N. ] / Plant Pathology(Fourth Edition)
39 Nutritional enhancement of biological control of blue mold on apples /
[ Janisiewicz,W.J.;J.Usall;B.Bors ] / Phytopathology   DOI
40 Pathogenicity of Bacillus circulans to seedings of date plam (Phoenix dactylifera0 /
[ Leary,J.V.;W.W.C.Chun ] / Plant Dis.   DOI
41 A versatile quick-prep of genomic DNA from Gram-positive bacteria /
[ Pospiech,A.;B.Neumann ] / Trends Gents.   DOI   ScienceOn
42 The plant growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: A Possible connection between biotic and abiotic stress response /
[ Timmusk,S.;E.G.H.Wagner ] / Mol. Plant Microbe Interact.   DOI   ScienceOn
43 Aerobic Spore-Forming Bacteria /
[ Smith,N.R.;R.E.Bordon;F.E.Clark ] / U.S. Dept. Agric. Monogr.
44 Production of a potentially novel anti-microbial substance by Bacillus polymyxa /
[ Rosado,A.S.;L.Seldin ] / J. Microbiol. Biotechnol.   DOI   ScienceOn