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Application of Denaturing Gradient Gel Electrophoresis to Estimate the Diversity of Commensal Thermophiles  

Bae, Jin-Woo (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Joong-Jae (BioLeaders Corp.)
Jeon, Che-Ok (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Kwang (BioLeaders Corp.)
Song, Jae-Jun (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Seung-Goo (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Poo, Har-Young (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Jung, Chang-Min (BioLeaders Corp.)
Park, Yong-Ha (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Sung, Moon-Hee (BioLeaders Corp., Department of Techno Sciecne, College of National Sciences, Kookmin University)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.6, 2003 , pp. 1008-1012 More about this Journal
Abstract
Symbiobacterium toebii has been reported as a thermophile exhibiting a commensal interaction with Geobacillus toebii. The distribution of the commensal thermophiles in various soils was investigated using a denaturing gradient gel electrophoresis (DGGE). Based on the DGGE analysis, the enrichment condition for the growth of Symbiobacterium sp. was found to also enrich populations of several other microbial spp. as well as Symbiobacterium sp. In the enrichment experiment, several different 16S rDNA sequences of commensal thermophiles were detected in all of the soil samples tested, indicating that commensal thermophiles are widely distributed in various soils.
Keywords
DGGE; diversity; Symbiobacterium toebii;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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