[KSCI] Korea Science Citation Index Service

Optimization of Programmed Suppression in a Cell-Free Protein Synthesis System with Unnatural Amino Acid S-(2-Nitrobenzyl)cysteine

HYUN JOO (Department of Molecular Science and Technology/Life Sciences, Ajou University)
KANG, TAEK JIN (School of Chemical Engineering, College of Engineering, Seoul National University)
HUI KYOUNG SONG (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University)
JIN HO AHN (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University)
CHA YONG CHOI (School of Chemical Engineering, College of Engineering, Seoul National University, Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University)

Publication Information

Journal of Microbiology and Biotechnology / v.13, no.3, 2003 , pp. 344-347 More about this Journal

Abstract

Unnatural amino acid S-(2-nitrobenzyl)cysteine was incorporated into human erythropoietin by using a programmed suppression of nonsense codon in a cell-free protein synthesis system. Several controlling factors affecting the operational efficiency of the suppression were investigated and optimized. The amount of suppressor tRNA and the concentration of $Mg^2+$ were crucial not only for the efficiency but also for the control of the exact suppression. In addition, some general optimization factor are reported in order to improve the efficiency in an unnatural amino acid mutagenesis.

Keywords

Cell-free protein synthesis; unnatural ammo acid mutagenesis; erythropoietin; suppressor tRNA; salt concentration optimization;

Citations & Related Records

Times Cited By Web Of Science : 3 (Related Records In Web of Science)

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