Browse > Article

Genotoxicity Assay Using Chromosomally-Integrated Bacterial recA::Lux  

Min, Ji-Ho (National Research Laboratory of Environmental Biotechnology, Kwangju Institute of Science and Technology(K-JIST))
Gu, Man-Bock (National Research Laboratory of Environmental Biotechnology, Kwangju Institute of Science and Technology(K-JIST))
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.1, 2003 , pp. 99-103 More about this Journal
Abstract
An Escherichia coli strain containing the recA promoter that fused to the luxCDABE operon originating from Photorhabdus luminescens was shown to respond sensitively to genotoxic stresses. Two different recombinant bacteria, one (DPDI 657) harboring a plasmid with the recA promoter that fused to the luxCDABE operon, and the other (DPD1710) containing a chromosomally-integrated recA promoter that fused with luxCDABE, were compared and it was found that the sensitivity of 'the two strains was significantly different in terms of their bioluminescent level, response time, and the minimum detectable concentration of a chemical causing DNA damaging stress. DPDI 710, with a chromosomally-integrated single copy, generally led to lower basal luminescence levels, faster responses, increased response ratios, and an enhanced sensitivity to mutagens, when compared to DPD 1657 with a multi-copy plasmid.
Keywords
RecA; chromosomal integration; genotoxicity; sensitivity;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
연도 인용수 순위
1 Construction and characterization of a recombinant bioluminescence Streptomycetes for potential environmental monitoring /
[ Park H. J.;K. O. Hwang;E. S. Kim ] / J. Microbiol. Biotechnol.   과학기술학회마을
2 Bacterial bioluminescent emission from recombinant Escherichia coli harboring a recA::luxCDABE fusion /
[ Gu M. B.;J. Min;R. A. LaRossa ] / J. Biochem. Biophys. Meth.   DOI   ScienceOn
3 Stabilization of bioluminescence of immobilized Photobacterium phosphoreum and monitoring of environmental pollutants /
[ Britz M. L.;N. Simonov;U. H. Chun ] / J. Microbiol. Biotechnol.   과학기술학회마을
4 Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents /
[ Min J.;E. J. Kim;R. A. LaRossa;M. B. Gu ] / Mutat. Res.   DOI   ScienceOn
5 Detection of DNA damage by use of Eschrichia coli carrying recA'::lux, uvrA'::lux, or AlkA'::lux reporter plasmids /
[ Vollmer A. C.;S. Belkin;D. R. Smulski;T. K. van Dyk;R. A. LaRossa ] / Appl. Env. Microbiol.
6 Stress responses of the Escherichia coli groE promoter /
[ Kwak Y. H.;J. K. Sung;Y. L. Ki;K. B. Han ] / J. Microbiol. Biotechnol.   과학기술학회마을
7 Improved bacterial SOS promoter::lux fusions for genotoxicity detection /
[ Yaakov D.;R. Rozen;D. R. Smulski;T. K. van Dyk,A. C. Vollmer;D. A. Elsemore;R. A. Larossa;S. Belkin ] / Mutat. Res.   DOI   ScienceOn
8 Characterization of plasmids from Bifidobacterium sp. /
[ Lee J. H.;M. S. Park;K. H. Lee;G. E. Ji ] / J. Microbiol. Biotechnol.   과학기술학회마을
9 Immobilized luminescent cell-based flow through monitoring of environmental pollutants /
[ Britz M. L.;N. Simonov;U. H. Chun ] / J. Microbiol. Biotechnol.   과학기술학회마을
10 The Vitotox test an SOS bioluminescence Salmonella typhimurium test to measure genotoxicity kinetics /
[ van der Lelie D.;L. Regniers;B. Borremans;A. Provoost;L. Verschaeve ] / Mutat. Res.   DOI   ScienceOn
11 Rapid detection of mutagens by induction of luciferase-bearing prophage in Escherichia coli. /
[ Karine J.;J. M. Mailiard;J. Benedik;R. C. Wilson ] / Environ. Sci. Technol.   DOI   ScienceOn
12 Toxicity monitoring of endocrine disrupting chemicals (EDCs) using freeze-dried recombinant bioluminescent bacteria /
[ Kim S. W.;S. H. Choi;J. Min;M. B. Gu ] / Biotech. Bioprocess Eng.   과학기술학회마을   DOI
13 Characterization of a cadmium-resistant yeast strain in response to cadmium or heat shock stress /
[ Huh N. E.;N. S. Choi;Y. K. Seo;T. S. Yu;H. S. Lee ] / J. Microbiol. Biotechnol.   과학기술학회마을
14 Methods for detecting carciogens and mutagens with the Salmonellal mammalian microsome mutagenicity test /
[ Ames B. N.;J. McCann;E. Yamasaki ] / Mutat. Res.   DOI
15 A biosensor for environmental genotoxin screening based on a SOS lux assay in recombinant Escherichia coli cells /
[ Ptitsynm L. R.;G. Horneck;O. Komova;S. Kozubek;E. A. Krasavin;M. Bonev;P. Rettberg ] / Appl. Env. Microbiol.
16 Toxicity monitoring and classification of Endocrine-Disrupting Chemicals (EDCs) using recombinant bioluminescent bacteria /
[ Gu M. B.;J. Min;E. J. Kim ] / Chemosphere   DOI   ScienceOn