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Increase of Conjugated Linoleic Acid Level in Milk Eat by Bovine Feeding Regimen and Urea Fractionation  

KIM, YOUNG JUN (Department of Molecular Biology and Genetics, Cornell University)
KI WON LEE (Department of Food Science and Technology, School of Agricultural biology, Seoul National University)
HYONG JOO LEE (Department of Food Science and Technology, School of Agricultural biology, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.1, 2003 , pp. 22-28 More about this Journal
Abstract
Increasing conjugated linoleic acid (CLA) content in dairy products has been a research Interest due to the potential health benefits resulted from consuming CLA. Attempts were made to obtain high level natural CLA containing fatty acid fractions from milk fat through bovine feeding of sunflower oil (SO) and urea fractionation. SO feeding changed the fatty acid profile of milk fat. increasing the CLA content five-fold at eight weeks of trial. Milk fat obtained from S0-fed cows was hydrolyzed to free fatty acids, which were then fractionated with urea at various ratios. The profiles of fatty acids were also greatly influenced by urea fractionation. Long-chain unsaturated fatty acids, Including CLA, were concentrated in milk fat after the fractionation, whereas saturated long-chain counterparts were eliminated. The highest level of CLA was achieved by the fractionation at 2:1 urea/fatty acid ratio (UFR2). CLA level was elevated 2.5-fold, and the Cl8:1/C18:0 fatty acid ratio was increased 120 times after the fractionation. The level of CLA in high CLA-milk fat (24mg/g fat) obtained from the feeding study was further increased through urea fractionation up to 52mg/g fat, 10 folds as high as CLA in the control milk fat (5mg/g fat).
Keywords
Conjugated linoleic acid; sunflower oil; urea; fractionation; milk fat;
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Times Cited By KSCI : 2  (Citation Analysis)
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