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Bacterial Community Composition of Activated Sludge Relative to Type and Efficiency of Municipal Wastewater Treatment Plants  

Ahn, In-Sook (Toxic and Refractory Wastwater Treatment Lab., Ecodigms Co. Ltd., EERC, KIST)
Kim, Myeong-Woon (Department of Environmental Engineering, Daejin University)
La, Hyun-Joon (Toxic and Refractory Wastwater Treatment Lab., Ecodigms Co. Ltd., EERC, KIST)
Choi, Kyung-Min (Toxic and Refractory Wastwater Treatment Lab., Ecodigms Co. Ltd., EERC, KIST)
Kwon, Joong-Cheon (Toxic and Refractory Wastwater Treatment Lab., Ecodigms Co. Ltd., EERC, KIST)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.1, 2003 , pp. 15-21 More about this Journal
Abstract
Two microbial communities of activated sludge in the same municipal wastewater, but treated with different systems, were studied and compared using molecular microbiological approaches. The bacterial 16S rDNA sequences from 124 clones were analyzed, however, the majority of them were not closely related to any known species, and found to belong to 8 different phylogenetic groups and 3 different unidentified groups. The relative frequencies of each group were similar between the two microbial communities. Fingerprinting using terminal restriction fragment length polymorphism (T-RFLP) showed that the putative Nitrospira-related populations were more diverse and quantitatively higher in the KNR process system than in the other system using a conventional activated sludge process. The relationship between the bacterial community composition and the higher removal efficiency of nitrogen and phosphorus in the KNR process is discussed.
Keywords
Activated sludge; bacterial community; T-RFLP; 16S rDNA; phylogeny;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Optimization of terminal-restriction fragment length polymorphism analysis for complex marine bacterioplankton communities and comparison with denaturing gradient gel electrophoresis /
[ Moeseneder M. M.;J. M. Arrieta;G. Muyzer;C. Winter;G. J. Herndl ] / Appl. Environ. Microbiol.
2 Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation /
[ Crocetti G. R.;P. Hugenholtz;P. L. Bond;A. Schuler;J. Keller;D. Jenkins;L. L. Blackall ] / Appl. Environ. Microbiol.   DOI   ScienceOn
3 Bioaugmentation of sequencing batch reactor for biological phoshphorus removal: Comparative rRNA sequence analysis and hybridization with oligonucleotide probes /
[ Oerther D. B.;J. Danalewich;E. Dulekgurgen;E. Leveque;D. L. Freedman;L. Raskin ] / Wat. Sci. Tech.   DOI   ScienceOn
4 Basic local alignment search tool /
[ Altschul S. F.;W. Miller;E. W. Meyers;D. J. Limpman ] / J. Mol. Biol.
5 CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice /
[ Thompson J. D.;D. G. Higgins;T. J. Gibson ] / Nucleic Acids Res   DOI   ScienceOn
6 Determination of microbial diversity in environmental samples: Pitfalls of PCR-based rRNA analysis /
[ Wintzingerode F. V.;U. B. Gobel;E. Stackebrandt ] / FEMS Microbiol. Rev.   DOI   ScienceOn
7 Identification of some of the major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems /
[ Bond P. L.;R. Erhart;M. Wagner;J. Keller;L. L. Blackall ] / Appl. Environ. Microbiol.
8 Molecular analysis of ammonia oxidation and denitrification in natural environments /
[ Bothe H.;G. Jost;M. Schloter;B. B. Ward;K. Witzel ] / FEMS Microbiol. Rev.   DOI   ScienceOn
9 Phylogenetic specificity and reproducibility and new method for analysis of terminal restriction fragment profiles of 16S rRNA genes from bacterial community /
[ Dunbar J.;L. O. Ticknor;C. R. Kuske ] / Appl. Environ. Microbiol.   DOI   ScienceOn
10 Microbial evolution, diversity and ecology: A decade of ribosomal RNA analysis of uncultivated microorganisms /
[ Head I. M.;J. R. Saunders;R. W. Pickup ] / Microb. Ecol.   DOI   ScienceOn
11 American Water Works Association and Water Environment Federation /
[ American Public Health Association;A. D. Eaton (ed.);L. S. Clesceri (ed.);A. E. Greenberg (ed.) ] / Standard Methods for the Examination of Water and Wastewater(19th ed.)
12 Association of marine archaea with the digestive tracts of two marine fish species /
[ van der Maarel;M. J. E. C.;R. R. E. Artz;R. Haanstra;L. J. Forney ] / Appl. Environ. Microbiol.
13 Distribution and ecophysiology of the nitrifying bacteria emphasizing cultured species /
[ Koops H. P.;A. P. Roser ] / FEMS Microbiol. Ecol.   DOI   ScienceOn
14 Detection and cultivation of filamentous bacteria from activated sludge /
[ Kamper P. ] / FEMS Microbiol. Ecol.   DOI   ScienceOn
15 Isolation and identification of photosynthetic bacterium useful for wastewater treatment /
[ Choi H. P.;H. J. Kang;H. C. Seo;H. C. Sung ] / J. Microbiol. Biotechnol.   과학기술학회마을
16 The neighbor-joining method: A new method for reconstructing phylogenetic trees /
[ Saitou N.;M. Nei ] / Mol. Biol. Evol.
17 Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor /
[ Nielsen A. T.;W. T. Liu;C. Filipe;L. Grady Jr.;S. Molin;D. A. Stahl ] / Appl. Environ. Microbiol.
18 PHYLIP Phylogeny Inference Package (version 3.2) /
[ Felsenstein J. ] / Cladistics
19 /
[ Bitton G. ] / Wastewater Microbiology
20 Identification of cultureable oligotrophic bacteria within naturally occurring bacterioplankton communities of the Ligurian Sea by 16S rRNA sequencing and probing /
[ Giuliano L.;M. De Domenico;E. De Domenico;M. G. Hofle;M. M. Yakimov ] / Microb. Ecol.   DOI   ScienceOn
21 Molecular characterization of nocardioform actinomycetes in activated sludge by 16S rRNA analysis /
[ Schuppler M.;F. Mertens;G. Schon;U. B. Gobel ] / Microbiology   DOI   ScienceOn
22 Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA /
[ Liu W. T.;T. L. Marsh;H. Cheng;L. J. Forney ] / Appl. Environ. Microbiol.
23 16S/23S rRNA sequencing /
[ Lane D. J.;E. Stackebrandt (ed.);M. Goodfellow (ed.) ] / Nucleic Acid Techniques in Bacterial Systematics
24 Therminal restriction fragment patterns (TRFLPs), a rapid, PCR-based method for the comparison of complex bacterial communities /
[ Clement B. G.;L. E. Kehl;K. L. DeBord;C. L. Kitts ] / J. Microbiol. Methods   DOI   ScienceOn
25 Thermophilic bacterial communities in hot composts as revealed by most probable number counts and molecular (16S rDNA) methods /
[ Blanc M.;L. Marilley;T. Beffa;M. Aragno ] / FEMS Microbiol. Ecol.   DOI   ScienceOn
26 Characterization of TCE-degrading bacteria and their application to wastewater treatment /
[ Lee W. S.;C. S. Park;J. E. Kim;B. D. Yoon;H. M. Oh ] / J. Microbiol. Biotechnol.   과학기술학회마을
27 Community analysis of ammonia-oxidising bacteria in relation to oxygen availability in soils and root-oxygenated sediments, using PCR, DGGE and oligonucleotide probe hydridisation /
[ Kowalchuk G. A.;P. L. E. Bodelier;G. H. J. Heilig;J. R. Stephen;H. J. Laanbroek ] / FEMS Microbiol. Ecol.   DOI   ScienceOn
28 Terminal restriction fragment length polymorphism analysis program, a web-based research tool for microbial community analysis /
[ Marsh T. L.;P. Saxman;J. Cole;J. Tiedje ] / Appl. Environ. Microbiol.   DOI   ScienceOn