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Overproduction of Bacillus macerans Cyclodextrin Glucanotransferase in E. coli by Coexpression of GroEL/ES Chaperone  

Kwon, Mi-Jung (Department of Microbiology, Dong-Eui University)
So-Lim Park (Department of Microbiology, Dong-Eui University)
Sung-Koo Kim (Department of Biotechnology & Bioengineering, Pukyong National University)
Soo-Wan Nam (Department of Biotechnology & Bioengineering, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.6, 2002 , pp. 1002-1005 More about this Journal
Abstract
The effects of GroEL/ES chaperone on the production of soluble form of B. macerans cyclodextrin glucanotransferase (CGTase) in recombinant E. coli were investigated. The cgt gene and groEL/ES genes are under the control of T7 promoter and Pzt-1 promoter, respectively. The optimal concentrations of inducers, IPTG and tetracycline, were found to be 1.0 mM and 10 ng/ml, respectively. When tetracycline and IPTG were added at the early exponential phase (2h) and exponential phase (3h) of growth, respectively, about 1.5-fold increase of soluble CGTase activity and 1.6-fold increase of soluble CGTase protein were obtained. An SDS-PAGE analysis revealed that about $37.2\%$ of total CGTase protein was in the soluble fraction when GroEL/ES chaperone was overexpressed.
Keywords
Coexpression; cyclodextrin glucanotransferase; GroEL/ES chaperone; Escherichia coli;
Citations & Related Records
Times Cited By KSCI : 11  (Citation Analysis)
Times Cited By Web Of Science : 16  (Related Records In Web of Science)
연도 인용수 순위
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