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Characterization of the rfaD Gene Region of Bradyrhizobium japonicum 61A101C

Noh, Jae-Sang (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University)
Kim, Dong-Hyun (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University)
Oh, Eun-Taex (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University)
So, Jae-Seong (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University)

Publication Information

Journal of Microbiology and Biotechnology / v.12, no.5, 2002 , pp. 826-828 More about this Journal

Abstract

In our previous studies, we have cloned and characterized a gene region from Bradyrhizobium japonicum ,which is involved in the synthesis of lipopolysaccharide (LPS). In this study, we have expanded the sequence analysis of the region and found an additional open reading frame (orf), which appeared to be divergently transcribed from the rfaF gene. Sequence alignment of the orf revealed a significant similarity with rfaD genes of Salmonella typhimurium , Escherichia coli, and Neisseria gonorrhoeae. These genes encode a heptose-6-epimerase, which catalyzes the interconversion of ADP -D -glycerol-D-manno-heptose to ADP-L-glycero-D-manno-heptose. This divergent organization of the rfaF and rfaD genes is different from that of other Gram-negative bacteria where two genes form an operon. A rfaD- mutant of E. coli was successfully transformed with plasmid constructs containing the rfaD gene of B. japonicum. Novobiocin sensitivity test showed that the rfaD gene from B. japonicum could complement the rfaD mutation in E. coli, which confirms the functionality of the cloned B. japonicum gene.

Keywords

Bradyrhizobium japonicum; lipopolysaccharide(LPS); rfaD; complementation; novobiocin;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)
Times Cited By Web Of Science : 2 (Related Records In Web of Science)

Reference
Cited By KSCI

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