Browse > Article

Physico-Chemical and Rheological Properties of a Bioflocculant BF-56 from Bacillus sp. A56  

Suh, Hyun-Hyo (Department of Environmental Engineering, Chinju National University)
Moon, Seong-Hoon (Biomolecular Process Engineering Laboratory)
Seo, Weon-Taek (Department of Food Processing, Chinju National University)
Kim, Kyung-Kab (Department of Environmental Engineering, Chinju National University)
Jeon, Gee-Ill (Department of Environmental Engineering, Chinju National University)
Park, Hyun-Geoun (Department of Environmental Engineering, Chinju National University)
Park, Yong-Il (Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.2, 2002 , pp. 209-216 More about this Journal
Abstract
Bacillus sp. A56 was studied, because of its high flocculating activity. The flocculating substance produced by this strain was purified by ethanol precipitation, cetylpyridinium chloride (CPC) precipitation, and gel permeation chromatography (GPC). The FT-IR spectrum of the purified bioflocculant, designated as BF-56, showed typical characteristics of polysaccharides. The non-sugar substituents, and sugar components of BF-56 containing glucose, fucose, glucuronic acid, and galactose in an approximate molar ratio of 2.76:1.10:1:0.12, suggested that it was a novel bioflocculant with an estimated molecular mass of over $7{\times}10^3$ kDa. Rheological analysis of BF-56 revealed that it was a pseudoplastic that had higher apparent viscosity rate at dilute concentrations than those of zooglan. The solution of bioflocculant BF-56 exhibited non-Newtonian characteristics and it was compatible to high concentrations of salts such as KCl, NaCl, $CaCl_2,\;or\;FeCl_3.$ The present results suggested strong possibility of bioflocculant BF-56 to be fully applicable to industries such as wastewater treatment.
Keywords
Novel bioflocculant; bacterial polysaccharide; rheology;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248- 254   DOI   ScienceOn
2 Harada, T., M. Terasaki, and A. Harada. 1993. Polysaccharides and their derivatives, pp. 427-445. In R. L. Whistler and J. N. BeMiller (eds.), Industrial Gums. Academic Press, New York, U.S.A
3 Nakamura, J., S. Miyashiro, and Y. Hirose. 1976. Conditions for production of microbial cell flocculant by Aspergillus sojae AJ7002. Agric. Biol. Chem. 40: 1341-1347
4 Pavia, D. L., G. M. Lampman, and G. S. Kriz. 1996. Introduction to Spectroscopy, pp. 17-83. 2th ed. Saunders Golden Sunburst Series, Saunders College Publishing, Florida, U.S.A
5 Pasika, W. M. 1977. Polysaccharide polyelectrolytes, pp. 128-143. In P. A. Standford (ed.), Extracellular Microbial Polysaccharides, ACS symposium series 45. American Chemical Society
6 McComb, E. A. and R. M. McCready. 1957. Determination of acetyl in pectin and in acetylated carbohydrate polymers. Anal. Chem. 29: 819-821
7 Attia, Y. A. 1987. Design of selective polymers for biological and colloid separation by selective flocculation, pp. 227- 246. In Y. A. Attia (ed.), Flocculation in Biotechnology and Separation Systems, vol 4. Elsevier, New York, U.S.A
8 Michaels, A. S. 1954. Aggregation of suspensions by polyelectrolytes. Ind. Eng. Chem. 46: 1485
9 Kurane, R., K. Takeda, and T. Suzuki. 1986. Screening for and characteristics of microbial flocculants. Agr. Biol. Chem. 50: 2301-2307
10 Ahn, S. G., H. H. Suh, C. H. Lee, S. H. Moon, H. S. Kim, K. H. Ahn, G. S. Kwon, H. M. Oh, and B. D. Yoon. 1998. Isolation and characterization of a novel polysaccharide producing Bacillus polymyxa A49 KCTC 4648P. J. Microbiol. Biotechnol. 8: 171-177
11 Unz, R. F. and S. R. Farrah. 1976. Exopolymer production and flocculation by Zoogloea MP6. Appl. Environ. Microbiol. 31: 623-626
12 Takeda, M., R. Kurane, J. Koizumi, and I. Nakamura. 1991. A protein bioflocculant produced by Rhodococcus erythropolis. Agr. Biol. Chem. 55: 2663-2664
13 Zajic, J. E. and E. Knettig. 1970. Flocculants from Paraffinic Hydrocarbons, pp. 87-98. American Institute of Biological Science, Washington DC, U.S.A
14 Chaplin, M. F. and J. F. Kennedy. 1986. Carbazole assay, pp. 5. In M. F. Chaplin and J. F. Kennedy (eds.), Carbohydrate Analysis - A Practical Approach, IRL Press, Washington DC, U.S.A
15 Aouadi, S., A. Heyraud, F. Segle-Murandi, R. Steiman, and B. Founrnet. 1992. Structural analysis and rheological behaviour of extracellular polysaccharide from Drechslera spicifera. Carbohydr. Polym. 17: 177-183
16 Chaplin, M. F. and J. F. Kennedy. 1986. Phenol-sulfuric acid assay, pp. 2. In M. F. Chaplin and J. F. Kennedy (eds.), Carbohydrate Analysis - A Practical Approach, IRL Press, Washington DC, U.S.A
17 Dearfield, K. L. and C. O. Ambermathy. 1988. Acrylamideits metabolism, developmental and reproductive effects, genotoxicity, and carcinogenicity. Mutant Res. 195: 45-77
18 Toeda, K. and R. Kurane. 1991. Microbial flocculant from Alcaligenes cupidus KT201. Agr. Biol. Chem. 55: 2793- 2799
19 Gutcho, S. 1977. Waste Treatment with Polyelectrolytes and Other Flocculants, pp. 1-37. Noyes Data Corporation, Park Ridge, New Jersey, U.S.A
20 Peter, H. A. S., S. M. Nicholas, M. E. Sharpe, and J. G. Holt. 1986. Bergey's Manual of Systematic Bacteriology, pp. 1104-1139. vol. 2. Williams and Wilkins Co., Baltimore, U.S.A
21 Suh, H. H., M. H. Lee, H. S. Kim, C. S. Park, and B. D. Yoon. 1993. Bioflocculant-producing Bacillus sp. A56. Kor. J. Appl. Microbiol. Biotechnol. 21: 486-493
22 Chaplin, M. F. and J. F. Kennedy. 1986. Carbazole assay, pp. 5. In M. F. Chaplin and J. F. Kennedy (eds.), Carbohydrate Analysis - A Practical Approach, IRL Press, Washington DC, U.S.A
23 Moon, S. H., S. D. Hong, G. S. Kwon, H. H. Suh, H. S. Kim, K. H. Ahn, H. M. Oh, T. I. Mheen, and B. D. Yoon. 1998. Determination of medium components in the flocculation activity and production of pestan produced by Pestalopsis sp. by using the Plackett-Burman design. J. Microbiol. Biotechnol. 8: 341-346
24 Nohata, Y. and R. Kurane. 1993. Culture conditions for production and purification of bioabsorbent from Acaligenes latus B-16. J. Ferment. Bioeng. 77: 390-393
25 Seo, W. T., G. G. Kahng, S. H. Nam, S. D. Choi, H. H. Suh, S. W. Kim, and Y. H. Park. 1999. Isolation and characterization of a novel exopolysaccharide-producing Penibacillus sp. WN9 KCTC 8591P. J. Microbiol. Biotechnol. 9: 820-825
26 Takagi, H. and K. Kadowaki. 1985. Flocculant production by Pacilomyces sp. Taxonomic studies and culture conditions for production. Agr. Biol. Chem. 49: 3151-3157
27 Frideman, T. E. and G. E. Haugen. 1943. The determination of keto acids in blood and urine. Pyruvic acid, II. J. Biol. Chem. 147: 415-442