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Cellulase-Free Thermostable Alkaline Xylanase from Thermophilic and Alkalophilic Bacillus sp. JB-99  

Naik, G.R. (Department of Biotechnology, Gulbarga University)
Johnvesly, B. (Department of Biotechnology, Gulbarga University)
Virupakshi, S. (Department of Biochemistry, Gulbarga University)
Patil, G.N. (Department of Biochemistry, Gulbarga University)
Ramalingam (Department of Biochemistry, Gulbarga University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.1, 2002 , pp. 153-156 More about this Journal
Abstract
The characterization of a partially purified, cellulase-free, thermostable alkaline xylanase from thermoalkalophilic Bacillus sp. JB-99 was investigated. The xylanase production was the highest when birchwood xylan was added to a medium containing finely powdered rice bran, showing 4,826 IU$ml^-1$ of activity for 15 h of incubation. The partially purified xylanase exhibited an optimum temperature and pH at $70^C{\circ}$ and 10, respectively. The enzyme was stable at pH 5-11 at $50^C{\circ}$. The xylanase activity was strongly inhibited by $Hg^2+$, while dithiothreitol, cysteine, and ${\beta}$-mercaptoethanol enhanced the activity.
Keywords
Bacillus sp.; biobleaching; cellulase-free xylanase; thermoalkalophilic;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 25  (Related Records In Web of Science)
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