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http://dx.doi.org/10.1080/12298093.2020.1807454

A New Species of Thelonectria and a New Record of Cephalotrichum hinnuleum from Gunwi and Ulleungdo in Korea  

Das, Kallol (College of Agriculture and Life Sciences, Kyungpook National University)
You, Young-Hyun (Microorganism Resources Division, National Institute of Biological Resources)
Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University)
Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Mycobiology / v.48, no.5, 2020 , pp. 341-350 More about this Journal
Abstract
Three fungal strains belonging to the class Sordariomycetes were isolated from soils collected from Gyeongsangbuk-do in Korea. They were identified as Cephalotrichum hinnuleum (UD CT 1-3-3 and KNU-19GWF1) and Thelonectria chlamydospora sp. nov. (UD ST 1-2-1). T. chlamydospora sp. nov. was morphologically identical to T. truncata, but its specific macroconidial dimensions, lower number of septations, and chlamydospore diameter render it distinct from the strains of the genus Thelonectria. The strains UD CT 1-3-3 and KNU-19GWF1 were developed flat, velvety to felty, and golden gray to brown-gray after 14 days of incubation at 25 ℃ on PDA. These strains were produced polyblastic conidiogenous cells and conidia were pale brown to brown, smooth, thin-walled, subglobose to ellipsoidal, arranged in chains, and the diameters of 6.7-9.0 × 3.7-5.1 ㎛. The strains were also confirmed by using the multi-locus genes using internal transcribed spacer (ITS) regions, partial large subunit (LSU), translation elongation factor 1α (TEF1-α), β-tubulin (TUB2), and actin (ACT) genes. This is the discovery of T. chlamydospora sp. nov. and Cephalotrichum hinnuleum, a new record from Korea.
Keywords
Cephalotrichum hinnuleum; Sordariomycetes; Thelonectria chlamydospora;
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1 White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press, Inc.; 1990. p. 315-322.
2 Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2(2):113-118.   DOI
3 Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 1990;172(8):4238-4246.   DOI
4 Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia. 2005;97(1):84-98.   DOI
5 Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61(4):1323-1330.   DOI
6 Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16(2):111-120.   DOI
7 Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874.   DOI
8 Flannigan B, Samson RA, Miller JD (eds.). Microorganisms in home and indoor work environments. Diversity, health impacts, investigation and control. 2nd ed. Florida, USA: CRC press; 2011.
9 Sabev HA, Handley PS, Robson GD. Fungal colonization of soil-buried plasticized polyvinyl chloride (pPVC) and the impact of incorporated biocides. Microbiology. 2006;152(Pt 6):1731-1739.   DOI
10 Jiang YL, Xu JJ, Wu YM, et al. Studies on Cephalotrichum from soils in China-twelve new species and two new combinations. Mycotaxon. 2011;117(1):207-225.   DOI
11 Pangging M, Nguyen TTT, Lee HB. New records of four species belonging to Eurotiales from soil and freshwater in Korea. Mycobiology. 2019;47(2):154-164.   DOI
12 Heo I, Hong K, Yang H, et al. Diversity of Aspergillus, Penicillium, and Talaromyces species isolated from freshwater environments in Korea. Mycobiology. 2019;47(1):12-19.   DOI
13 Brayford D, Honda BM, Mantiri FR, et al. Neonectria and Cylindrocarpon: the Nectria mammoidea group and species lacking microconidia. Mycologia. 2004;96(3):572-597.   DOI
14 Hsieh HM, Chou JC, Ju YM. Nectriaceous fungi collected from forests in Taiwan. Bot Stud. 2020;61(1):187-203.
15 Lu BS, Hyde KD, Ho WH. Checklist of Hong Kong fungi. Fungal Diversity Series 5. Hong Kong: Fungal Diversity Press; 2000.
16 Zhuang WY, Nong Y, Luo J. New species and new Chinese records of bionectriaceae and nectriaceae (Hypocreales, Ascomycetes) from Hubei, China. Fungal Divers. 2007;24:347-357.
17 Carlucci A, Lops F, Mostert L, et al. Occurrence fungi causing black foot on young grapevines and nursery rootstock plants in Italy. Phytopathol Mediterr. 2017;56:10-39.
18 Zeng ZQ, Zhuang WY. The genera Rugonectria and Thelonectria (Hypocreales, Nectriaceae) in China. MycoKeys. 2019;55:101-120.   DOI
19 Zeng ZQ, Zhuang WY. Four new taxa of Ilyonectria and Thelonectria (Nectriaceae) revealed by morphology and combined ITS and b-tubulin sequence data. Phytotaxa. 2013;85(1):15-25.
20 Salgado-Salazar C, Rossman AY, Samuels GJ, et al. Phylogeny and taxonomic revision of Thelonectria discophora (Ascomycota, Hypocreales, Nectriaceae) species complex. Fungal Divers. 2015;70(1):1-29.   DOI
21 Domsch KH, Gams W, Anderson TH. Compendium of soil fungi. 2nd ed. Eching. Germany: IHW Verlag; 2007.
22 Maharachchikumbura SSN, Hyde KD, Jones EB, et al. Families of Sordariomycetes. Fungal Divers. 2016;79(1):1-317.   DOI
23 Malloch D. New concepts in the Microascaceae illustrated by two new species. Mycologia. 1970;62(4):727-740.   DOI
24 Abbott SP. Holomorph studies of the Microascaceae [Ph.D. dissertation]. Canada: University of Alberta; 2000.
25 Rossman AY, Samuels GJ, Rogerson CT, et al. Genera of bionectriaceae, hypocreaceae and nectriaceae (Hypocreales, Ascomycetes). Stud Mycol. 1999;42:1-248.
26 Sun BY, Shin H, Hyun JO, et al. Vascular plants of Dokdo and Ulleungdo islands in Korea. Incheon, Korea: National Institute of Biological Resources; 2014.
27 Chaverri P, Salgado-Salazar C, Hirooka Y, et al. Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon-like anamorphs. Stud Mycol. 2011;68:57-78.   DOI
28 Salgado-Salazar C, Rossman A, Samuels GJ, et al. Multigene phylogenetic analyses of the Thelonectria coronata and T. veuillotiana species complexes. Mycologia. 2012;104(6):1325-1350.   DOI
29 Lee BH, Kim DY, Park H, et al. Notes on endophytic fungi isolated from roots of Oreorchis patens in Korea. Kor J Mycol. 2016;44:184-187.   DOI
30 Paul NC, Mun HY, Lee HW, et al. A new record of Penicillium raphiae isolated from agricultural soil of Ulleung Island, Korea. Mycobiology. 2014;42(3):282-285.   DOI
31 Lee HY, Nguyen TTT, Mun HY, et al. Confirmation of two undescribed fungal species from Dokdo of Korea based on current classification system using multi loci. Mycobiology. 2015;43(4):392-401.   DOI
32 Lee SH, Park HS, Nguyen TTT, et al. Characterization of three species of Sordariomycetes isolated from freshwater and soil samples in Korea. Mycobiology. 2019;47(1):20-30.   DOI
33 Sandoval-Denis M, Guarro J, Cano-Lira1 JF, et al. Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi. Stud Mycol. 2016;83:193-233.   DOI
34 Nirenberg HI, Aoki T. Fusarium nisikadoi, a new species from Japan. Mycoscience. 1997;38(3):329-333.   DOI
35 Zeng ZQ, Zhuang WY. Three new Chinese records of Nectriaceae. Mycosystema. 2016;35:1399-1405.