Browse > Article
http://dx.doi.org/10.1080/12298093.2019.1651592

Identification and Antifungal Susceptibility Profiles of Cyberlindnera fabianii in Korea  

Park, Ji-Hyun (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Oh, Junsang (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Sang, Hyunkyu (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Shrestha, Bhushan (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Lee, Hyeyoung (Department of Laboratory Medicine, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Koo, Jehyun (Department of Laboratory Medicine, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Cho, Sung-Il (Department of Laboratory Medicine, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Choi, Ji Seon (Department of Laboratory Medicine, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Lee, Min-Ha (Department of Laboratory Medicine, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Kim, Jayoung (Department of Laboratory Medicine, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Sung, Gi-Ho (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Publication Information
Mycobiology / v.47, no.4, 2019 , pp. 449-456 More about this Journal
Abstract
Invasive fungal infections caused by Cyberlindnera fabianii have recently increased. However, biochemical kits such as API 20 C AUX and Vitek-2C have misidentified this species as other Candida spp. such as C. pelliculosa or C. utilis due to no information of Cy. fabianii in yeast database. During our 2016-2017 surveys, eleven isolates of Cy. fabianii were obtained in International St. Mary's Hospital in Korea. Here, we describe its morphological and molecular characteristics and tested its antifungal susceptibility against nine antifungal agents. The sequences of the ITS region and the D1/D2 region of LSU revealed 100% identity with the sequences of Cy. fabianii. In comparison with the results from MALDI-TOF mass spectrometry, we found that Cy. fabianii can be distinguished from other species. In antifungal susceptibility test, voriconazole and echinocandins exhibited good antifungal activities against the majority of Cy. fabianii isolates despite the absence of standard criteria.
Keywords
Antifungal susceptibility test; Cyberlindnera fabianii; MALDI-TOF MS; MIC; phylogenetic analysis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Baghdadi J, Hemarajata P, Humphries R, et al. First report of ventriculoperitoneal shunt infection due to Cyberlindnera fabianii. Case Rep Infect Dis. 2015;630816:1-6.
2 Jindal N, Arora S, Dhuria N, et al. Cyberlindnera (Pichia) fabianii infection in a neutropenic child: importance of molecular identification. JMM Case Rep. 2015;2:1-3. http://dx.doi.org/10.1099/jmmcr.0.000033.
3 Hof H, Amann V, Tauber C, et al. Peritonitis in a neonate due to Cyberlindnera fabianii, an ascomycetic yeast. Infection. 2017;45:921-924.   DOI
4 Bhally HS, Jain S, Shields C, et al. Infection in a neonate caused by Pichia fabianii: importance of molecular identification. Med Mycol. 2006;44:185-187.   DOI
5 Gabriel F, Noel T, Accoceberry I. Lindnera (Pichia) fabianii blood infection after mesenteric ischemia. Med Mycol. 2012;50:310-314.   DOI
6 Valenza G, Valenza R, Brederlau J, et al. Identification of Candida fabianii as a causal of lethal septicaemia. Mycoses. 2006;49:331-334.   DOI
7 Zhao Y, Tsang CC, Xiao M, et al. Yeast identification by sequencing, biochemical kits, MALDI-TOF MS and rep-PCR DNA fingerprinting. Med Mycol. 2018;56:816-827.   DOI
8 Haas M, Grenouillet F, Loubersac S, et al. Identification of cryptic Candida species by MALDI-TOF mass spectrometry, not all MALDITOF systems are the same: focus on the C. parapsilosis species complex. Diagn Microbial Infect Dis. 2016;84:385-386.
9 Ghosh AK, Paul S, Sood P, et al. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the rapid identification of yeasts causing bloodstream infections. Clin Microbiol Infect. 2015;21:372-378.   DOI
10 Richard MJ, Edwards JR, Culver DH, et al. Nosocomial infections in medical intensive care units in the United States. National Nosocomial Infections Surveillance System. Crit Care Med. 1999;27:887-892.   DOI
11 Kauffman CA. Candiduria. Clin Infect Dis. 2005;41:S371-S376.   DOI
12 Nicolle LE. Catheter associated urinary tract infections. Antimicrob Resist Infect Control. 2014;3:23.   DOI
13 Kaur R, Dhakad MS, Goyal R, et al. Emergence of non-albicans Candida species and antifungal resistance in intensive care unit patients. Asian Pac J Trop Biomed. 2016;6:455-460.   DOI
14 Bukhary ZA. Candiduria: a review of clinical significance and management. Saudi J Kidney Dis Transpl. 2008;19:350-360.
15 Yun JW, Park KS, Ki CS, et al. Catheter-related bloodstream infection by Lindnera fabianii in a neutropenic patient. J Med Microbiol. 2013;62:922-925.   DOI
16 Svobodova L, Bednarova D, Ruzicka F, et al. High frequency of Candida fabianii among clinical isolates biochemically identified as Candida pelliculosa and Candida utilis. Mycoses. 2016;59:241-246.   DOI
17 Grenouillet F, Millon L, Chamouine A, et al. Pichia fabianii fungemia in a neonate. Pediatr Infect Dis J. 2010;29:191.   DOI
18 Wu Y, Wang J, Li W, et al. Pichia fabianii blood infection in a premature infant in China: case report. BMC Res Notes. 2013;6:77.   DOI
19 Lee JI, Yu S, Park JS, et al. Successful treatment of fungemia caused by Cyberlindnera fabianii with Anidulafungin: a case report. Ann Clin Microbiol. 2015;18:94-97.   DOI
20 Mlinaric-Missoni E, Hatvani L, Kocsube S, et al. Cyberlindnera fabianii in the neonatal and paediatric intensive care unit: case reports. JMM Case Rep. 2015;2:1-6. https://doi.org/10.1099/jmmcr.0.000032.
21 Tamura K, Stecher G, Peterson D, et al. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725-2729.   DOI
22 Kim YJ, Yang JJ, Lee HJ. Possibility of frequent detection of invasive Cyberlindnera fabianii infection using molecular method. Ann Clin Microbiol. 2015;18:133-134.   DOI
23 White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego, CA, USA: Academic Press; 1990. p. 315-322.
24 O'Donnell K. Fusarium and its near relatives In: Reynolds DR, Taylor JW, editors. The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. Wallingford, United Kingdom: CAB International; 1993. p. 529-580.
25 Clinical and Laboratory Standards Institute (CLSI). M27-S4 Reference method for broth dilution antifungal susceptibility testing of yeasts: fourth informational supplement M27-S4. Wayne, PA: Clinical and Laboratory Standards Institute; 2012.
26 Minter DW. Cyberlindnera, a replacement name for Lindnera kurtzman et al., nom. illegit. Mycotaxon. 2009;110:473-476.   DOI
27 Hamal P, Ostransky J, Dendis M, et al. A case of endocarditis caused by the yeast Pichia fabianii with biofilm production and developed in vitro resistance to azoles in the course of antifungal treatment. Med Mycol. 2008;46:601-605.   DOI