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http://dx.doi.org/10.1080/12298093.2019.1625854

1H-NMR Analysis of Metabolic Changes Induced by Snf1/AMP-Activated Protein Kinase During Environmental Stress Responses  

Kim, Jiyoung (Jeonju AgroBio-Materials Institute)
Oh, Junsang (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Yoon, Deok-Hyo (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Sung, Gi-Ho (Translational Research Division, Biomedical Institute of Mycological Resource, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Publication Information
Mycobiology / v.47, no.3, 2019 , pp. 346-349 More about this Journal
Abstract
AMP-activated protein kinase sucrose non-fermenting 1 (Snf1) is a representative regulator of energy status that maintains cellular energy homeostasis. In addition, Snf1 is involved in the mediation of environmental stress such as salt stress. Snf1 regulates metabolic enzymes such as acetyl-CoA carboxylase, indicating a possible role for Snf1 in metabolic regulation. In this article, we performed nuclear magnetic resonance (NMR) spectroscopy to profile the metabolic changes induced by Snf1 under environmental stress. According to our NMR data, we suggest that Snf1 plays a role in regulating cellular concentrations of a variety of metabolites during environmental stress responses.
Keywords
Snf1/AMPK; stress signals; nuclear magnetic resonance; metabolic changes; yeast;
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1 Pastor MM, Proft M, Pascual-Ahuir A. Mitochondrial function is an inducible determinant of osmotic stress adaptation in yeast. J Biol Chem. 2009;284:30307-30317.   DOI
2 Woods A, Munday MR, Scott J, et al. Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl-CoA carboxylase in vivo. J Biol Chem. 1994;269:19509-19515.   DOI
3 Beckonert O, Keun HC, Ebbels TM, et al. Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts. Nat Protoc. 2007;2:2692-2703.   DOI
4 Kim HK, Choi YH, Verpoorte R. NMR-based metabolomic analysis of plants. Nat Protoc. 2010;5:536-549.   DOI
5 Kim J, Oh J, Sung GH. MAP kinase Hog1 regulates metabolic changes induced by hyperosmotic stress. Front Microbiol. 2016;7:732.
6 Hounsa CG, Brandt EV, Thevelein J, et al. Role of trehalose in survival of Saccharomyces cerevisiae under osmotic stress. Microbiology. 1998;144:671-680.   DOI
7 Olz R, Larsson K, Adler L, et al. Energy flux and osmoregulation of Saccharomyces cerevisiae grown in chemostats under NaCl stress. J Bacteriol. 1993;175:2205-2213.   DOI
8 Oren A. Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev. 1999;63:334-348.   DOI
9 Hardie DG, Carling D, Carlson M. The AMP-activated/ SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell? Annu Rev Biochem. 1998;67:821-855.   DOI
10 Hardie DG. AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy. Nat Rev Mol Cell Biol. 2007;8:774-785.   DOI
11 McCartney RR, Schmidt MC. Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit. J Biol Chem. 2001;276:36460-36466.   DOI
12 Kayikci O, Nielsen J. Glucose repression in Saccharomyces cerevisiae. FEMS Yeast Res. 2015;15:fov068.   DOI
13 Thompson-Jaeger S, Francois J, Gaughran JP, et al. Deletion of SNF1 affects the nutrient response of yeast and resembles mutations which activate the adenylate cyclase pathway. Genetics. 1991;129:697-706.   DOI
14 Alepuz PM, Cunningham KW, Estruch F. Glucose repression affects ion homeostasis in yeast through the regulation of the stress-activated ENA1 gene. Mol Microbiol. 1997;26:91-98.   DOI
15 Dubacq C, Chevalier A, Mann C. The protein kinase Snf1 is required for tolerance to the ribonucleotide reductase inhibitor hydroxyurea. Mol Cell Biol. 2004;24:2560-2572.   DOI
16 Portillo F, Mulet JM, Serrano R. A role for the non-phosphorylated form of yeast Snf1: tolerance to toxic cations and activation of potassium transport. FEBS Lett. 2005;579:512-516.   DOI
17 Hong SP, Carlson M. Regulation of snf1 protein kinase in response to environmental stress. J Biol Chem. 2007;282:16838-16845.   DOI
18 Hedbacker K, Carlson M. SNF1/AMPK pathways in yeast. Front Biosci. 2008;13:2408-2420.   DOI