Browse > Article
http://dx.doi.org/10.1080/12298093.2018.1468054

Alternaria brassicifolii sp. nov. Isolated from Brassica rapa subsp. pekinensis in Korea  

Deng, Jian Xin (Department of Plant Protection, College of Agriculture, Yangtze University)
Li, Mei Jia (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agriculture Sciences)
Paul, Narayan Chandra (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Oo, May Moe (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Lee, Hyang Burm (Division of Applied Bioscience & Biotechnology, College of Agriculture & Life Sciences, Chonnam National University)
Oh, Sang-Keun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Yu, Seung Hun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Mycobiology / v.46, no.2, 2018 , pp. 172-176 More about this Journal
Abstract
A new species belonging to the genus Alternaria was isolated from the necrotic leaf spots of Brassica rapa subsp. pekinensis in Yuseong district, Daejeon, Korea. It is an occasional isolate, not an etiological agent, which is morphologically similar to A. broccoli-italicae, but differs in conidial size and conidiophore shape. Phylogenetic analysis using the sequence datasets of the internal transcribed spacer (ITS) region of the rDNA, glyceraldehyde-3-phosphate dehydrogenase (gpd), and plasma membrane ATPase genes showed that it is distantly related to A. broccoli-italicae and closely related to Alternaria species in the section Pseudoalternaria, which belonged to a clade basal to the section Infectoriae. Morphologically, the species is unique because it produces solitary conidia or conidial chains (two units), unlike the four members in the section Pseudoalternaria that produce conidia as short branched chains. It exhibits weak pathogenicity in the host plant. This report includes the description and illustration of A. brassicifolii as a new species.
Keywords
Brassicaceae; morphology; pathogenicity; phylogenetic analysis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Thompson JD, Gibson TJ, Plewniak F, et al. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;25:4876-4882.   DOI
2 Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol. 2008;57:758-771.   DOI
3 Tamura K, Peterson D, Peterson N, et al. MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Bio Evol. 2011;28:2731-2739.   DOI
4 Gannibal PB, Lawrence DP. Distribution of Alternaria species among sections. 3. Sections Infectoriae and Pseudoalternaria. Mycotaxon. 2016;131:781-790.   DOI
5 Yu SH. Alternaria species and allied genera. Incheon: National Institute of Biological Resources Ministry of Environment; 2015.
6 Nishikawa J, Nakashima C. Taxonomic characterization and experimental host ranges of four newly recorded species of Alternaria from Japan. J Phytopathol. 2013;161:604-616.   DOI
7 Kumar D, Maurya N, Bharati YK, et al. Alternaria blight of oilseed Brassicas: a comprehensive review. Afr J Microbiol Res. 2014;8:2816-2829.   DOI
8 Simmons EG. Alternaria: an identification manual. CBS Biodiversity Series 6. Utrecht: Centraalbureau voor Schimmelcultures; 2007.
9 Andersen B, Kroger E, Roberts RG. Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes. Mycol Res. 2001;105:291-299.   DOI
10 Zhang TY. Flora fungorum sinicorum, vol. 16 Alternaria. Beijing: Science Press; 2003.
11 Pryor BM, Bigelow DM. Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium. Mycologia. 2003;95:1141-1154.   DOI
12 Hong SG, Cramer RA, Lawrence CB, et al. Alt a 1 allergen homologs from Alternaria and related taxa: analysis of phylogenetic content and secondary structure. Fungal Genet Biol. 2005;42:119-129.   DOI
13 Park MS, Romanoski CE, Pryor BM. A re-examination of the phylogenetic relationship between the causal agents of carrot black rot, Alternaria radicina and A. carotiincultae. Mycologia. 2008;100:511-527.   DOI
14 Nowicki M, Nowakowska M, Niezgoda A, et al. Alternaria black spot of crucifers: symptoms, importance of disease, and perspectives of resistance breeding. Veg Crops Res Bull. 2012;76:5-19.
15 Toth B, Csosz M, Szabo-Hever A, et al. Alternaria hungarica sp. nov., a minor foliar pathogen of wheat in Hungary. Mycologia. 2011;103:94-100.   DOI
16 Lawrence DP, Rotondo F, Gannibal PB. Biodiversity and taxonomy of the pleomorphic genus Alternaria. Mycol Prog. 2016;15:3.   DOI
17 Deng JX, Cho HS, Paul NC, et al. A novel species belonging to Alternaria isolated from Peucedanum japonicum in Korea. Mycobiology. 2014;42:12-16.   DOI
18 Woudenberg J, Truter M, Groenewald J, et al. Large-spored Alternaria pathogens in section Porri disentangled. Stud Mycol. 2014;79:1-47.   DOI
19 Woudenberg JH, Groenewald JZ, Binder M, et al. Alternaria redefined. Stud Mycol. 2013;75:171-212.   DOI
20 Park MS, Seo GS, Bae KS, et al. Characterization of Trichoderma spp. associated with green mold of Oyster mushroom by PCR-RFLP and sequence analysis of ITS regions of rDNA. Plant Pathol J. 2005;21:229-236.   DOI
21 White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-322.
22 Berbee ML, Pirseyedi M, Hubbard S. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia. 1999;91:964-977.   DOI
23 Poursafar A, Ghosta Y, Orina AS, et al. Taxonomic study on Alternaria sections Infectoriae and Pseudoalternaria associated with black (sooty) head mold of wheat and barley in Iran. Mycol Progress. 2018;17:343-356.   DOI
24 Lawrence DP, Gannibal PB, Dugan FM, et al. Characterization of Alternaria isolates from the infectoria species-group and a new taxon from Arrhenatherum, Pseudoalternaria arrhenatheria sp. nov. Mycol Progress. 2014;13:257-276.   DOI