Browse > Article
http://dx.doi.org/10.1080/12298093.2018.1454007

Identification and Characterization of Alternaria iridiaustralis Causing Leaf Spot on Iris ensata in China  

Luo, Huan (Department of Plant Protection, College of Agriculture, Yangtze University)
Tao, Ya Qun (Department of Plant Protection, College of Agriculture, Yangtze University)
Fan, Xiao Yan (Department of Plant Protection, College of Agriculture, Yangtze University)
Oh, Sang Keun (Department of Applied Biology, Chungnam National University)
Lu, Hong Xue (Department of Plant Protection, College of Agriculture, Yangtze University)
Deng, Jian Xin (Department of Plant Protection, College of Agriculture, Yangtze University)
Publication Information
Mycobiology / v.46, no.2, 2018 , pp. 168-171 More about this Journal
Abstract
In 2016, a severe leaf spot disease was found on Iris ensata Thumb. in Nanjing, China. The symptom was elliptical, fusiform, or irregularly necrotic lesion surrounded by a yellow halo, from which a small-spored Alternaria species was isolated. The fungus was identified as Alternaria iridiaustralis based on morphological characteristics. The pathogenicity tests revealed that the fungus was the causal pathogen of the disease. Phylogenic analyses using sequences of ITS, gpd, endoPG, and RPB2 genes confirmed the morphological identification. This study is the first report of A. iridiaustralis causing leaf spots on I. ensata in China.
Keywords
Alternaria iridiaustralis; Iris ensata Thumb; morphology; pathogenicity; sequence analysis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Wilson CA. Subgeneric classification in Iris reexamined using chloroplast sequence data. Taxon. 2011;60:27-35.
2 Wang H, Cui Y, Zhao C. Flavonoids of the genus Iris (Iridaceae)). Mini Rev Med Chem. 2010;10:643-661.   DOI
3 Orhan I, Nasim S, Sener B, et al. Two isoflavones and bioactivity spectrum of the crude extracts of Iris germanica rhizomes. Phytother Res. 2003;17:575-577.   DOI
4 Zheng Y, Meng T, Bi X, et al. Investigation and evaluation of wild Iris resources in Liaoning Province, China. Genet Resour Crop Evol. 2017;64:967-978.   DOI
5 Zhang TY. Flora fungorum sinicorum, vol. 16 Alternaria. Beijing: Science Press; 2003.
6 Simmons EG. Alternaria: an identification manual, CBS biodiversity series 6. Utrecht: Centraalbureau voor Schimmelcultures; 2007.
7 Yu SH. Alternaria and allied genaera. Suwon:National Institute of Biological Resources Ministry of Environment; 2015.
8 Berbee ML, Pirseyedi M, Hubbard S. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia. 1999;91:964-977.   DOI
9 Wu FH, Huang DY, Huang XL, et al. Comparing study on several methods for DNA extraction from endophytic fungi. Chin Agric Sci Bull. 2009;25:62-64.
10 White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, et al., editors. PCR protocols: a guide to methods and applications. San Diego (CA):Academic Press; 1990. p. 315-322.
11 Woudenberg JH, Seidl MF, Groenewald JZ, et al. Alternaria section Alternaria: species, formae speciales or pathotypes? Stud Mycol. 2015;82:1-21.   DOI
12 Andrew M, Peever TL, Pryor BM. An expanded multilocus phylogeny does not resolve morphological species within the small-spored Altemrnaria species complex. Mycologia. 2009;101:95-109.   DOI
13 Liu YJ, Whelen S, Hall BD. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Mol Biol Evol. 1999;16:1799-1808.   DOI
14 Woudenberg JH, Groenewald JZ, Binder M, et al. Alternaria redefined. Stud Mycol. 2013;75:171-212.   DOI
15 Larkin MA, Blackshields G, Brown NP, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23:2947-2948.   DOI
16 Tamura K, Peterson D, Peterson N, et al. MEGA5:Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2007;28:2731-2739.
17 Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006;22:2688-2690.   DOI