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http://dx.doi.org/10.5941/MYCO.2016.44.1.48

Ethanol Production from Various Sugars and Cellulosic Biomass by White Rot Fungus Lenzites betulinus  

Im, Kyung Hoan (Division of Life Sciences, Incheon National University)
Nguyen, Trung Kien (Division of Life Sciences, Incheon National University)
Choi, Jaehyuk (Division of Life Sciences, Incheon National University)
Lee, Tae Soo (Division of Life Sciences, Incheon National University)
Publication Information
Mycobiology / v.44, no.1, 2016 , pp. 48-53 More about this Journal
Abstract
Lenzites betulinus, known as gilled polypore belongs to Basidiomycota was isolated from fruiting body on broadleaf dead trees. It was found that the mycelia of white rot fungus Lenzites betulinus IUM 5468 produced ethanol from various sugars, including glucose, mannose, galactose, and cellobiose with a yield of 0.38, 0.26, 0.07, and 0.26 g of ethanol per gram of sugar consumed, respectively. This fungus relatively exhibited a good ethanol production from xylose at 0.26 g of ethanol per gram of sugar consumed. However, the ethanol conversion rate of arabinose was relatively low (at 0.07 g of ethanol per gram sugar). L. betulinus was capable of producing ethanol directly from rice straw and corn stalks at 0.22 g and 0.16 g of ethanol per gram of substrates, respectively, when this fungus was cultured in a basal medium containing 20 g/L rice straw or corn stalks. These results indicate that L. betulinus can produce ethanol efficiently from glucose, mannose, and cellobiose and produce ethanol very poorly from galactose and arabinose. Therefore, it is suggested that this fungus can ferment ethanol from various sugars and hydrolyze cellulosic materials to sugars and convert them to ethanol simultaneously.
Keywords
Cellulosic biomass; Ethanol; Lenzites betulinus; White rot fungus;
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1 Naik SN, Goud VV, Rout PK, Dalai AK. Production of first and second generation biofuels: a comprehensive review. Renew Sust Energ Rev 2010;14:578-97.   DOI
2 Sun Y, Cheng J. Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 2002;83:1-11.   DOI
3 Piskur J, Rozpedowska E, Polakova S, Merico A, Compagno C. How did Saccharomyces evolve to become a good brewer? Trends Genet 2006;22:183-6.   DOI
4 du Preez JC, van der Walt JP. Fermentation of D-xylose to ethanol by a strain of Candida shehatae. Biotechnol Lett 1983;5:357-62.   DOI
5 Jeffries TW. Conversion of xylose to ethanol under aerobic conditions by Candida tropicalis. Biotechnol Lett 1981;3:213-8.   DOI
6 Toivola A, Yarrow D, van den Bosch E, van Dijken JP, Scheffers WA. Alcoholic fermentation of D-xylose by yeasts. Appl Environ Microbiol 1984;47:1221-3.
7 Suihko ML, Enari TM. The production of ethanol from Dglucose and D-xylose by different Fusarium strains. Biotechnol Lett 1981;3:723-8.   DOI
8 Deshpande V, Keskar S, Mishra C, Rao M. Direct conversion of cellulose/hemicellulose to ethanol by Neurospora crassa. Enzyme Microb Technol 1986;8:149-52.   DOI
9 Mountfort DO, Rhodes LL. Anaerobic growth and fermentation characteristics of Paecilomyces lilacinus isolated from mullet gut. Appl Environ Microbiol 1991;57:1963-8.
10 Okamura T, Ogata T, Minamimoto N, Takeno T, Noda H, Fukuda S, Ohsugi M. Characteristics of wine produced by mushroom fermentation. Biosci Biotechnol Biochem 2001;65: 1596-600.   DOI
11 Maehara T, Ichinose H, Furukawa T, Ogasawara W, Takabatake K, Kaneko S. Ethanol production from high cellulose concentration by the basidiomycete fungus Flammulina velutipes. Fungal Biol 2013;117:220-6.   DOI
12 Horisawa S, Ando H, Ariga O, Sakuma Y. Direct ethanol production from cellulosic materials by consolidated biological processing using the wood rot fungus Schizophyllum commune. Bioresour Technol 2015;197:37-41.   DOI
13 Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D. Determination of structural carbohydrates and lignin in biomass. Laboratory Analytical Procedure (LAP) [Internet]. Golden (CO): National Renewable Energy Laboratory; 2008 [cited 2016 Mar 1]. Available from: http://www.nrel.gov/biomass/analytical procedure.html.
14 Park WH, Lee JH. New wild fungi in Korea. Seoul: Kyohak Publishing Co; 2011. p. 319.
15 White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego (CA): Academic Press; 1990. p. 315-22.
16 Okamoto K, Imashiro K, Akizawa, Y, Onimura A, Yoneda M, Nitta Y, Maekawa N, Yanase H. Production of ethanol by the white-rot basidiomycetes Peniophora cinerea and Trametes suaveolens. Biotechnol Lett 2010;32:909-13.   DOI
17 Miller GL. Use of dinitrosalicyIic acid reagent for determination of reducing sugar. Anal Chem 1959;31:426-8.   DOI
18 Skory CD, Freer SN, Bothast RJ. Screening for ethanolproducing filamentous fungi. Biotechnol Lett 1997;19:203-6.   DOI
19 Millati R, Edebo L, Taherzadeh MJ. Performance of Rhizopus, Rhizomucor, and Mucor in ethanol production from glucose, xylose, and wood hydrolyzates. Enzyme Microb Technol 2005;36:294-300.   DOI
20 Puls J, Schuseil J. Chemistry of hemicellulose: relationship between hemicellulose structure and enzymes required for hydrolysis. In: Coughlan MP, Hazlewood GP, editors. Hemicelluloses and hemicellulases. London: Portland Press; 1993. p. 1-27.
21 Liang X, Hua D, Wang Z, Zhang J, Zhao Y, Xu H, Li Y, Gao M, Zhang X. Production of bioethanol using lignocellulosic hydrolysate by the white rot fungus Hohenbuehelia sp. ZW-16. Ann Microbiol 2013;63:719-23.   DOI
22 Sanchez S, Bravo V, Castro E, Moya AJ, Camacho F. The fermentation of mixtures of D-glucose and D-xylose by Candida shehatae, Pichia stipitis or Pachysolen tannophilus to produce ethanol. J Chem Technol Biotechnol 2002;77:641-8.   DOI
23 Rasmussen ML, Shrestha P, Khanal SK, Pometto AL 3rd, Hans van Leeuwen J. Sequential saccharification of corn fiber and ethanol production by the brown rot fungus Gloeophyllum trabeum. Bioresour Technol 2010;101:3526-33.   DOI
24 Mizuno R, Ichinose H, Maehara T, Takabatake K, Kaneko S. Properties of ethanol fermentation by Flammulina velutipes. Biosci Biotechnol Biochem 2009;73:2240-5.   DOI
25 Kamei I, Hirota Y, Mori T, Hirai H, Meguro S, Kondo R. Direct ethanol production from cellulosic materials by the hypersaline-tolerant white-rot fungus Phlebia sp. MG-60. Bioresour Technol 2012;112:137-42.   DOI
26 Okamoto K, Nitta Y, Maekawa N, Yanase H. Direct ethanol production from starch, wheat bran and rice straw by the white rot fungus Trametes hirsuta. Enzyme Microb Technol 2011;48:273-7.   DOI