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http://dx.doi.org/10.5941/MYCO.2015.43.1.43

Chemical Constituents of the Culture Broth of Phellinus linteus and Their Antioxidant Activity  

Lee, Myeong-Seok (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University)
Hwang, Byung Soon (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University)
Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University)
Seo, Geon-Sik (Korea National College of Agriculture and Fisheries)
Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University)
Publication Information
Mycobiology / v.43, no.1, 2015 , pp. 43-48 More about this Journal
Abstract
The medicinal fungus Phellinus linteus, in the family Hymenochaetaceae, has been used as a traditional medicine for the treatment of various diseases. In this study, the chemical constituents of the culture broth of P. linteus were investigated. P. linteus was cultured in potato dextrose broth medium, and the culture broth was extracted with ethyl acetate. The ethyl acetate-soluble portion was concentrated and subjected to ODS column chromatography, followed by Sephadex LH-20 column chromatography. Six compounds (1~6) were purified by preparative reversed-phase high-performance liquid chromatography. Spectroscopic methods identified their structures as caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), phellilane H (4), (2E,4E)-(+)-4'-hydroxy-${\gamma}$-ionylideneacetic acid (5), and (2E,4E)-${\gamma}$-ionylideneacetic acid (6). Compounds 1, 2, and 3 exhibited potent dose-dependent antioxidant activity.
Keywords
Antioxidant; Caffeic acid; Inotilone; Medicinal fungus; Phellinus linteus;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Jung JY, Lee IK, Seok SJ, Lee HJ, Kim YH, Yun BS. Antioxidant polyphenols from the mycelial culture of the medicinal fungi Inonotus xeranticus and Phellinus linteus. J Appl Microbiol 2008;104:1824-32.   DOI
2 Kim HG, Yoon DH, Lee WH, Han SK, Shrestha B, Kim CH, Lim MH, Chang W, Lim S, Choi S, et al. Phellinus linteus inhibits inflammatory mediators by suppressing redox-based NF-kappaB and MAPKs activation in lipopolysaccharideinduced RAW 264.7 macrophage. J Ethnopharmacol 2007;114:307-15.   DOI
3 Shon MY, Kim TH, Sung NJ. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem 2003;82:593-7.   DOI
4 Deng K, Zhang Y, Ren Z, Xie L, Peng W, Gan B. Simultaneous determination of five fatty acids in Phellinus sp. by high-performance liquid chromatography with photodiodearray detection. J Med Plants Res 2011;5:2816-21.
5 Zhu T, Kim SH, Chen CY. A medicinal mushroom: Phellinus linteus. Curr Med Chem 2008;15:1330-5.   DOI
6 Yeo WH, Hwang EI, So SH, Lee SM. Phellinone, a new furanone derivative from the Phellinus linteus KT&G PL-2. Arch Pharm Res 2007;30:924-6.   DOI
7 Lee IK, Yun BS. Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance. J Antibiot (Tokyo) 2011;64:349-59.   DOI
8 Zheng YB, Lu CH, Shen YM. New abscisic acid-related metabolites from Phellinus vaninii. J Asian Nat Prod Res 2012;14:613-7.   DOI
9 Yeom JH, Lee IK, Ki DW, Lee MS, Seok SJ, Yun BS. Neuraminidase inhibitors from the culture broth of Phellinus linteus. Mycobiology 2012;40:142-4.   DOI
10 Lee IK, Han MS, Lee MS, Kim YS, Yun BS. Styrylpyrones from the medicinal fungus Phellinus baumii and their antioxidant properties. Bioorg Med Chem Lett 2010;20:5459-61.   DOI
11 Ichinohe T, Ainai A, Nakamura T, Akiyama Y, Maeyama J, Odagiri T, Tashiro M, Takahashi H, Sawa H, Tamura S, et al. Induction of cross-protective immunity against influenza A virus H5N1 by an intranasal vaccine with extracts of mushroom mycelia. J Med Virol 2010;82:128-37.   DOI
12 Lee YS, Kang YH, Jung JY, Lee S, Ohuchi K, Shin KH, Kang IJ, Park JH, Shin HK, Lim SS. Protein glycation inhibitors from the fruiting body of Phellinus linteus. Biol Pharm Bull 2008;31:1968-72.   DOI
13 Ferreira IC, Baptista P, Vilas-Boas M, Barros L. Free radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chem 2007;100:1511-6.   DOI
14 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26:1231-7.   DOI
15 Cuendet M, Hostettmann K, Potterat O, Dyatmiko W. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv Chim Acta 1997;80:1144-52.   DOI
16 Lee IK, Jung JY, Kim YS, Rhee MH, Yun BS. p-Terphenyls from the fruiting bodies of Paxillus curtisii and their antioxidant properties. Bioorg Med Chem 2009;17:4674-80.   DOI
17 Huang GJ, Huang SS, Deng JS. Anti-inflammatory activities of inotilone from Phellinus linteus through the inhibition of MMP-9, NF-${\kappa}B$, and MAPK activation in vitro and in vivo. PLoS One 2012;7:e35922.   DOI
18 Hwang BS, Lee MS, Lee SW, Lee IK, Seo GS, Choi HJ, Yun BS. Neuraminidase inhibitors from the fermentation broth of Phellinus linteus. Mycobiology 2014;42:189-92.   DOI
19 Oritani T, Yamashita K. Isolation and structure of 4-hydroxygamma-ionylideneacetic acids from Cercospora cruenta, a fungus producing +-abscisic acid. Agric Biol Chem 1987;51:275-8.
20 Zhang HY, Sun YM, Wang XL. Substituent effects on O-H bond dissociation enthalpies and ionization potentials of catechols: a DFT study and its implications in the rational design of phenolic antioxidants and elucidation of structureactivity relationships for flavonoid antioxidants. Chemistry 2003;9:502-8.   DOI
21 Cho JY, Kwon YJ, Sohn MJ, Seok SJ, Kim WG. Phellinstatin, a new inhibitor of enoyl-ACP reductase produced by the medicinal fungus Phellinus linteus. Bioorg Med Chem Lett 2011;21:1716-8.   DOI
22 Zhang L, Cheng YX, Liu AL, Wang HD, Wang YL, Du GH. Antioxidant, anti-inflammatory and anti-influenza properties of components from Chaenomeles speciosa. Molecules 2010;15:8507-17.   DOI
23 Lee IK, Yun BS. Highly oxygenated and unsaturated metabolites providing a diversity of hispidin class antioxidants in the medicinal mushrooms Inonotus and Phellinus. Bioorg Med Chem 2007;15:3309-14.   DOI