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http://dx.doi.org/10.5941/MYCO.2014.42.4.322

Genome-Wide Identification and Characterization of Novel Laccase Genes in the White-Rot Fungus Flammulina velutipes  

Kim, Hong-Il (Department of Biomedical Chemistry, Konkuk University)
Kwon, O-Chul (Department of Biomedical Chemistry, Konkuk University)
Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Lee, Chang-Soo (Department of Biomedical Chemistry, Konkuk University)
Park, Young-Jin (Department of Biomedical Chemistry, Konkuk University)
Publication Information
Mycobiology / v.42, no.4, 2014 , pp. 322-330 More about this Journal
Abstract
The aim of this study was to identify and characterize new Flammulina velutipes laccases from its whole-genome sequence. Of the 15 putative laccase genes detected in the F. velutipes genome, four new laccase genes (fvLac-1, fvLac-2, fvLac3, and fvLac-4) were found to contain four complete copper-binding regions (ten histidine residues and one cysteine residue) and four cysteine residues involved in forming disulfide bridges, fvLac-1, fvLac-2, fvLac3, and fvLac-4, encoding proteins consisting of 516, 518, 515, and 533 amino acid residues, respectively. Potential N-glycosylation sites (Asn-Xaa-Ser/Thr) were identified in the cDNA sequence of fvLac-1 (Asn-454), fvLac-2 (Asn-437 and Asn-455), fvLac-3 (Asn-111 and Asn-237), and fvLac4 (Asn-402 and Asn-457). In addition, the first 19~20 amino acid residues of these proteins were predicted to comprise signal peptides. Laccase activity assays and reverse transcription polymerase chain reaction analyses clearly reveal that $CuSO_4$ affects the induction and the transcription level of these laccase genes.
Keywords
Copper sulfate; Flammulina velutipes; Genome; Laccase;
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