Browse > Article
http://dx.doi.org/10.5941/MYCO.2013.41.3.149

Production and Characterization of a New ${\alpha}$-Glucosidase Inhibitory Peptide from Aspergillus oryzae N159-1  

Kang, Min-Gu (Department of Biomedicinal Science and Biotechnology, Paichai University)
Yi, Sung-Hun (Korea Food Research Institute)
Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
Publication Information
Mycobiology / v.41, no.3, 2013 , pp. 149-154 More about this Journal
Abstract
An ${\alpha}$-glucosidase inhibitor was developed from Aspergillus oryzae N159-1, which was screened from traditional fermented Korean foods. The intracellular concentration of the inhibitor reached its highest level when the fungus was cultured in tryptic soy broth medium at $27^{\circ}C$ for five days. The inhibitor was purified using a series of purification steps involving ultrafiltration, Sephadex G-25 gel permeation chromatography, strong cation exchange solid phase extraction, reverse-phase high performance liquid chromatography, and size exclusion chromatography. The final yield of the purification was 1.9%. Results of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the purified ${\alpha}$-glucosidase inhibitor was a tri-peptide, Pro-Phe-Pro, with the molecular weight of 360.1 Da. The IC50 value of the peptide against ${\alpha}$-glucosidase activity was 3.1 mg/mL. Using Lineweaver-Burk plot analysis, the inhibition pattern indicated that the inhibitor acts as a mixed type inhibitor.
Keywords
${\alpha}$-Glucosidase inhibitory peptide; Anti-obesity; Aspergillus oryzae N159-1;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Shin MK, Han SH. Effects of lotus (Nelumbo nucifera Gaertner) leaf powder on lipid concentrations in rats fed high fat diet rats. Korean J Food Cult 2006;21:202-8.
2 Schwartz MW, Woods SC, Porte D Jr, Seeley RJ, Baskin DG. Central nervous system control of food intake. Nature 2000;404:661-71.   DOI
3 Carr KD, Park TH, Zhang Y, Stone EA. Neuroanatomical patterns of Fos-like immunoreactivity induced by naltrexone in food-restricted and ad libitum fed rats. Brain Res 1998; 779:26-32.   DOI   ScienceOn
4 Wurtman JJ, Wurtman RJ, Growdon JH, Henry P, Lipscomb A, Zeisel SH. Carbohydrate craving in obese people: suppression by treatments affecting serotoninergic transmission. Int J Eat Disord 1981;1:2-15.   DOI
5 Wurtman JJ, Wurtman RJ. D-fenfluramine selectively decreases carbohydrate but not protein intake in obese subjects. Int J Obes 1984;8 Suppl 1:79-84.
6 Heraief E, Burckhardt P, Wurtman JJ, Wurtman RJ. Tryptophan administration may enhance weight loss by some moderately obese patients on a protein-sparing modified fast (PSMF) diet. Int J Eat Disord 1985;4:281-92.   DOI
7 Trichopoulou A, Gnardellis C, Benetou V, Lagiou P, Bamia C, Trichopoulos D. Lipid, protein and carbohydrate intake in relation to body mass index. Eur J Clin Nutr 2002;56:37-43.   DOI   ScienceOn
8 Kersten S. Mechanisms of nutritional and hormonal regulation of lipogenesis. EMBO Rep 2001;2:282-6.   DOI   ScienceOn
9 Doucet E, Tremblay A. Food intake, energy balance and body weight control. Eur J Clin Nutr 1997;51:846-55.   DOI   ScienceOn
10 Flatt JP. McCollum Award Lecture, 1995: diet, lifestyle, and weight maintenance. Am J Clin Nutr 1995;62:820-36.   DOI
11 Miura M, Gomyo T. Influences of commercial spices on $\alpha$- amylase, $\alpha$-glucosidase. J Jpn Soc Food Technol 1996;43:157-63   DOI
12 Holman RR, Cull CA, Turner RC. A randomized doubleblind trial of acarbose in type 2 diabetes shows improved glycemic control over 3 years (U.K. Prospective Diabetes Study 44). Diabetes Care 1999;22:960-4.   DOI   ScienceOn
13 Reese ET, Parrish FW. Nojirimycin and d-glucono-1,5-lactone as inhibitors of carbohydrases. Carbohydr Res 1971;18:381-8.   DOI   ScienceOn
14 Fatmawati S, Shimizu K, Kondo R. Ganoderol B: a potent $\alpha$- glucosidase inhibitor isolated from the fruiting body of Ganoderma lucidum. Phytomedicine 2011;18:1053-5.   DOI   ScienceOn
15 Wehmeier UF, Piepersberg W. Biotechnology and molecular biology of the alpha-glucosidase inhibitor acarbose. Appl Microbiol Biotechnol 2004;63:613-25.   DOI   ScienceOn
16 Murai A, lwamura K, Takada M, Ogawa K, Usui T, Okumura J. Control of postprandial hyperglycaemia by galactosyl maltobionolactone and its novel anti-amylase effect in mice. Life Sci 2002;71:1405-15.   DOI   ScienceOn
17 Asano N, Oseki K, Tomioka E, Kizu H, Matsui K. Ncontaining sugars from Morus alba and their glycosidase inhibitory activities. Carbohydr Res 1994;259:243-55.   DOI   ScienceOn
18 Leonhardt M, Hrupka B, Langhans W. New approaches in the pharmacological treatment of obesity. Eur J Nutr 1999; 38:1-13.   DOI   ScienceOn
19 Van de Laar FA, Lucassen PL, Akkermans RP, Van de Lisdonk EH, Rutten GE, Van Weel C. Alpha-glucosidase inhibitors for patients with type 2 diabetes: results from a Cochrane systematic review and meta-analysis. Diabetes Care 2005;28:154-63.   DOI   ScienceOn
20 Do JH, Joo HK. $\alpha$-D-Glucosidase inhibitor from Streptomyces sp. (I) Identification of the strain. Korean J Appl Microbiol Bioeng 1989;17:202-6.
21 Kim HS, Lee JY, Hwang KY, Cho YS, Park YS, Kang KD, Seong SI. Isolation and Identification of Bacillus sp. producing $\alpha$-glucosidase Inhibitor 1-deoxynojirimycin. Korean J Microbiol Biotechnol 2011;39:49-55.
22 Kim SD, Nho HJ. Isolation and characterization of $\alpha$- glucosidase inhibitor from the fungus Ganoderma lucidum. J Micriobiol 2004;42:223-7.
23 Kim YM, Wang MH, Rhee HI. A novel $\alpha$-glucosidase inhibitor from pine bark. Carbohydr Res 2004;339:715-7.   DOI   ScienceOn
24 Ono Y, Hattori E, Fukaya Y, Imai S, Ohizumi Y. Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J Ethnopharmacol 2006;106:238-44.   DOI   ScienceOn
25 Kim KY, Nam KA, Kurihara H, Kim SM. Potent $\alpha$- glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 2008;69:2820-5.   DOI   ScienceOn
26 Bush K, Herny PR, Slusarchyk DS. Muraceins: muramyl peptides produced by Norcardia orientalis as angiotensinconverting enzyme inhibitor. I. Taxonomy, fermentation and biological properties. J Antibiot (Tokyo) 1984;37:330-5.   DOI
27 Bennett JW. Aspergillus and koji: history, practice and molecular biology. SIM News 2001;51:65-71.
28 Yang HJ, Kwon DY, Kim MJ, Kang S, Park S. Meju, unsalted soybeans fermented with Bacillus subtillis and Aspergilus oryzae, potentiates insulinotropic actions and improves hepatic insulin sensitivity in diabetic rats. Nutr Metab (Lond) 2012; 9:37.   DOI   ScienceOn
29 Jang IT, Kang MG, Yi SH, Lim SI, Kim HR, Ahn BH, Lee JS. Physiological functionality of nuruk, makgeolli and cheonggukjang made with fungi and bacteria isolated from Korea traditional fermented foods. Kor J Mycol 2012;40:164-73   DOI   ScienceOn
30 Marui J, Matsushita-Morita M, Tada S, Hattori R, Suzuki S, Amano H, Ishida H, Yamagata Y, Takeuchi M, Kusomoto K. Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji). Appl Microbiol Biotechnol 2012;93:655-9.   DOI