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http://dx.doi.org/10.5941/MYCO.2012.40.1.071

Taxonomy of $Ganoderma$ $lucidum$ from Korea Based on rDNA and Partial ${\beta}$-Tubulin Gene Sequence Analysis  

Park, Young-Jin (Department of Applied Biochemistry, Konkuk University)
Kwon, O-Chul (Department of Applied Biochemistry, Konkuk University)
Son, Eun-Suk (Department of Applied Biochemistry, Konkuk University)
Yoon, Dae-Eun (Department of Applied Biochemistry, Konkuk University)
Han, Woo-Ri-Ja-Rang (Department of Applied Biochemistry, Konkuk University)
Yoo, Young-Bok (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Lee, Chang-Soo (Department of Applied Biochemistry, Konkuk University)
Publication Information
Mycobiology / v.40, no.1, 2012 , pp. 71-75 More about this Journal
Abstract
In the present study, a phylogenetic analysis was undertaken based on the internal transcribed spacer (ITS) rDNA and partial ${\beta}$-tubulin gene sequence of the $Ganoderma$ species. The size of the ITS rDNA regions from different $Ganoderma$ species varied from 625 to 673 bp, and those of the partial ${\beta}$-tubulin gene sequence were 419 bp. Based on the results, a phylogenetic tree was prepared which revealed that Korean $Ganoderma$ $lucidum$ strains belong in a single group along with a $G.$ $lucidum$ strain from Bangladesh.
Keywords
${\beta}$-Tubulin; $Ganoderma$ $lucidum$; Internal transcribed spacer;
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1 Wu S, Guo X, Zhou X, Li X, Chen Y, Lin J. AFLP analysis of genetic diversity in main cultivated strains of Ganoderma spp. Afr J Biotechnol 2009;8:3448-54.
2 Zheng L, Jia D, Fei X, Luo X, Yang Z. An assessment of the genetic diversity within Ganoderma strains with AFLP and ITS PCR-RFLP. Microbiol Res 2009;164:312-21.   DOI   ScienceOn
3 Liu X, Yuan JP, Chung CK, Chen XJ. Antitumor activity of the sporoderm-broken germinating spores of Ganoderma lucidum. Cancer Lett 2002;182:155-61.   DOI   ScienceOn
4 Moncalvo JM, Wang HH, Hseu RS. Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia 1995;87:223-38.   DOI   ScienceOn
5 Smith BJ, Sivasithamparam K. Internal transcribed spacer ribosomal DNA sequence of five species of Ganoderma from Australia. Mycol Res 2000;104:943-51.   DOI   ScienceOn
6 Hibbett DS, Donoghue MJ. Progress towards a phylogenetic classification of the Polyporaceae through parsimony analysis of mitochondrial ribosomal sequences. Can J Bot 1995; 73(Suppl 1):S853-61.
7 Vilgalys R, Sun BL. Ancient and recent patterns of geographic speciation in the oyster mushroom Pleurotus revealed by phylogenetic analysis of ribosomal DNA sequences. Proc Natl Acad Sci U S A 1994;91:4599-603.   DOI   ScienceOn
8 Crawford AR, Bassam BJ, Drenth A, Maclean DJ, Irwin JA. Evolutionary relationships among Phytophthora species deduced from rDNA sequence analysis. Mycol Res 1996; 100:437-43.   DOI   ScienceOn
9 Anderson DL, Gibbs AJ, Gibson NL. Identification and phylogeny of spore-cyst fungi (Ascosphaera spp.) using ribosomal DNA sequences. Mycol Res 1998;102:541-7.   DOI   ScienceOn
10 Chillali M, Idder-Ighili H, Guillaumin JJ, Mohammed C, Escarmant BL, Botton B. Variation in the ITS and IGS regions of ribosomal DNA among the biological species of European Armillaria. Mycol Res 1998;102:533-40.   DOI   ScienceOn
11 Bae SC, Lee SW, Kim HJ, Park DS, Rhee IK. PCR amplification of ITS II region of rDNA for the classification of Ganoderma spp. RDA J Agric Sci 1995;37:182-8.
12 Moncalvo JM, Wang HF, Hseu RS. Gene phylogeny of the Ganoderma lucidum complex based on ribosomal sequences: comparison with traditional taxonomic characters. Mycol Res 1995;99:1489-99.   DOI   ScienceOn
13 Thon MR, Royse DJ. Partial ${\beta}$-tubulin gene sequences for evolutionary studies in the Basidiomycotina. Mycologia 1999;91:468-74.   DOI
14 Cao H, But PP, Shaw PC. Methodological studies on genomic DNA extraction and purification from plant drug materials. J Chin Pharm Sci 1998;7:130-7.
15 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.   DOI   ScienceOn
16 Moncalvo JM, Wang HF, Wang HH, Hseu RS. The use of rDNA nucleotide sequence data for species identification and phylogeny in the Ganodermataceae. In: Proceedings of Contributed Symposium 59A, B 5th International Mycological Congress; 1994 Aug 14-21; Vancouver, Canada. Taipei: National Taiwan University; 1995. p. 31-44.