Browse > Article
http://dx.doi.org/10.4489/MYCO.2011.39.1.026

Inhibition Effects of Silver Nanoparticles against Powdery Mildews on Cucumber and Pumpkin  

Lamsal, Kabir (Division of Bio-Resources Technology, Kangwon National University)
Kim, Sang-Woo (Division of Bio-Resources Technology, Kangwon National University)
Jung, Jin-Hee (Division of Bio-Resources Technology, Kangwon National University)
Kim, Yun-Seok (Division of Bio-Resources Technology, Kangwon National University)
Kim, Kyoung-Su (Department of Agricultural Biotechnology, Center for Fungal Genetic Resources and Center for Fungal Pathogenesis, Seoul National University)
Lee, Youn-Su (Division of Bio-Resources Technology, Kangwon National University)
Publication Information
Mycobiology / v.39, no.1, 2011 , pp. 26-32 More about this Journal
Abstract
Powdery mildew is one of the most devastating diseases in cucurbits. Crop yield can decline as the disease severity increases. In this study, we evaluated the effect of silver nanoparticles against powdery mildew under different cultivation conditions in vitro and in vivo. Silver nanoparticles (WA-CV-WA13B) at various concentrations were applied before and after disease outbreak in plants to determine antifungal activities. In the field tests, the application of 100 ppm silver nanoparticles showed the highest inhibition rate for both before and after the outbreak of disease on cucumbers and pumpkins. Also, the application of 100 ppm silver nanoparticles showed maximum inhibition for the growth of fungal hyphae and conidial germination in in vivo tests. Scanning electron microscope results indicated that the silver nanoparticles caused detrimental effects on both mycelial growth and conidial germination.
Keywords
Agricultural chemical; Inhibition effect; Powdery mildew; Silver nanoparticles;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Braun U, Cook RT, Inman AJ, Shin HD. The taxonomy of the powdery mildew fungi. In: Belanger RR, Bushnell WR, Dik AJ, Carver TL, editors. The powdery mildews. A comprehensive treatise. St. Paul: APS Press; 2002. p. 13-55.
2 Jarvis W, Gubler WG, Grove GG. Epidemiology of powdery mildews in agricultural ecosystems. In: Belanger RR, Bushnell WR, Dik AJ, Carver TL, editors. The powdery mildews. a comprenhensive treatise. St. Paul: APS Press; 2002. p. 169-99.
3 McGrath MT. Successful management of powdery mildew in pumpkin with disease threshold-based fungicide programs. Plant Dis 1996;80:910-6.   DOI   ScienceOn
4 Zitter TA, Hopkins DL, Thomas CE. Compendium of cucurbit diseases. St Paul: APS Press; 1996.
5 Bragg, PD, Rannie DJ. The effect of silver ions on the respiratory chain of Escherichia coli. Can J Microbiol 1974;20:883-9.   DOI   ScienceOn
6 Thurman RB, Gerba CP, Bitton G. The molecular mechanisms of copper and silver ion disinfection of bacteria and viruses. Crit Rev Environ Sci Technol 1989;18:295-315.   DOI
7 Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 2000;52:662-8.   DOI   ScienceOn
8 Mirkin CA, Taton TA. Semiconductors meet biology. Nature 2000;405:626-7.   DOI   ScienceOn
9 Richards RM. Antimicrobial action of silver nitrate. Microbios 1981;31:83-91.
10 Nel A, Xia T, Madler L, Li N. Toxic potential of materials at the nanolevel. Science 2003;311:622-7.
11 Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ. The bactericidal effect of silver nanoparticles. Nanobiotechnology 2005;16:2346-53.   DOI
12 Samuel U, Guggenbichler JP. Prevention of catheter related infections: the potential of a new nano-silver impregnated catheter. Int J Antimicrob Agents 2004;23(Suppl 1):S75-8.   DOI   ScienceOn
13 Hwang ET, Lee JH, Chae YJ, Kim YS, Kim BC, Sang BI, Gu MB. Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria. Small 2008;4:746-50.   DOI   ScienceOn
14 Storz G, Imlay JA. Oxidative stress. Curr Opin Microbiol 1999;2:188-94.   DOI   ScienceOn