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http://dx.doi.org/10.4489/MYCO.2010.38.2.137

Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide  

Pham, Tuan Anh (Department of Bioscience and Biotechnology, The University of Suwon)
Kim, Jeong-Jun (Applied Entomology Division, National Academy of Agricultural Science)
Kim, Keun (Department of Bioscience and Biotechnology, The University of Suwon)
Publication Information
Mycobiology / v.38, no.2, 2010 , pp. 137-143 More about this Journal
Abstract
The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, $25^{\circ}C$ culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of $10^7$ conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.
Keywords
Beauveria bassiana; Conidia production; Optimal culture conditions; Solid-state fermentation;
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