[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4489/MYCO.2010.38.1.070

Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains

Shi, Shanliang (Department of Microbiology and Research Institute of Life Science, Gyeongsang National University)
Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Science, Gyeongsang National University)

Publication Information

Mycobiology / v.38, no.1, 2010 , pp. 70-73 More about this Journal

Abstract

Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over $30^{\circ}C$ . Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from $30^{\circ}C$ to $37^{\circ}C$ . Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.

Keywords

Cell cycle; Genomic DNA library; Pleurotus eryngii; Temperature sensitive; Yeast;

Citations & Related Records

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