[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4489/MYCO.2009.37.4.277

Screening of Trichoderma Isolates as a Biological Control Agent against Ceratocystis paradoxa Causing Pineapple Disease of Sugarcane

Rahman, M.A. (Department of Botany, University of Rajshahi)
Begum, M.F. (Department of Botany, University of Rajshahi)
Alam, M.F. (Department of Botany, University of Rajshahi)

Publication Information

Mycobiology / v.37, no.4, 2009 , pp. 277-285 More about this Journal

Abstract

In this study, dual culture, poison agar, and direct methods were used to assess the ability of Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433, and T. harzianum IMI-392434 to control Ceratocystis paradoxa, which causes the pineapple disease of sugarcane. The highest percentage inhibition of radial growth (PIRG) values were observed with T. harzianum IMI-392432 using two dual culture methods, 63.80% in Method I and 80.82% in Method II. The minimum colony overgrowth time was observed with T. harzianum IMI-392432 and the maximum was observed with T. pseudokoningii IMI-392431. Different concentrations of different day-old metabolites of Trichoderma isolates were tested against mycelial growth of C. paradoxa. The highest PIRG (84.685%) exhibited at 80% concentration of 30-day-old metabolites of T. harzianum IMI-392432 using the modified bilayer poison agar method. In the direct assay method the maximum mycelial growth weight (PIGW) was observed at the same concentration and the same day-old metabolites of T. harzianum IMI-392432. This study showed that Trichoderma isolates have a good antagonistic effect on C. paradoxa mycelial growth and T. harzianum IMI-392432 has the most potential to control the pineapple disease pathogen.

Keywords

Ceratocystis paradoxa; PIGW; PIRG; Secondary metabolites; Trichoderma;

Citations & Related Records

Reference

1	Baker, R. and Paulitz, T. C. 1996. Theoretical basis for microbial interactions leading to biological control of soilborne plant pathogens. In: Principles and Practice of Managing Soilborne Plant Pathogens, pp. 50-79. Ed. R. Hall. The American Phytopathological Society, St. Paul
2	Burgess, D. R. and Hepworth, G. 1996. Biocontrol of sclerotinia stem rot (Sclerotinia minor) in sunflower by seed treatment with Gliocladium virens. Plant Pathol. 45:583-592 DOI ScienceOn
3	Calistru, C., McLean, M. and Berjak, P. 1997. In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species: a study of the production of extracellular metabolites by Trichoderma species. Mycopathologia 137:115-124 DOI ScienceOn
4	Dharmaputra, O. S., Purba, R. Y. and Sipayung, A. 1994. Research activities on the biology and control of Ganoderma at SEAMEO BIOTROP and IOPRI Marihat. In: Proceedings of First International Workshop on Perennial Crop Diseases Caused by Ganoderma. Ed. M. Holderness. Universiti Pertanian Malaysia, Selangor
5	Doi, S. and Mori, M. 1994. Antifungal properties of metabolites produced by Trichoderma isolates from sawdust media of edible fungi against wood decay fungi. 28:143-151
6	Edgerton, C. W. 1959. Sugarcane and Its Diseases, pp. 290. Louisiana State University Press, Baton Rouge
7	Elad, Y. 1994. Biological control of grape grey mold by Trichoderma harzianum. Crop Prot. 13:35-38 DOI ScienceOn
8	Etabarian, H. R. 2006. Evaluation of Trichoderma isolates for biological control of charcoal stem rot in melon caused by Macrophomina phaseolina. J. Agric. Sci. Technol. 8:243-250
9	Eziashi, E. I., Omamor, I. B. and Odigie, E. E. 2007. Antagonism of Trichoderma viride and effects of extracted water soluble compounds from Trichoderma species and benlate solution on Ceratocystis paradoxa. Afr. J. Biotechnol. 6:388-392
10	Ghisalberti, E. L. and Rowland, C. Y. 1993. Antifungal metabolites from Trichoderma harzianum. J. Nat. Prod. 56:1799-1804 DOI ScienceOn
11	Jinantara, J. 1995. Evaluation of Malaysian isolates of Trichoderma harzianum Rifai and Glicocladium virens Miller, Giddens and Foster for the biological control of Sclerotium foot rot of chilli. Ph.D. Thesis. Universiti Putra Malaysia, Selangor, Malaysia
12	Schoeman, M. W., Webber, J. F. and Dickinson, D. J. 1996. The effect of diffusible metabolites of Trichoderma harzianum on in vitro interactions between basidiomycete isolates at two different temperature regimes. Mycol. Res. 100:1454-1458 DOI ScienceOn
13	KucuK, C. and Kivanc, M. 2003. Isolation of Trichoderma spp. and determination of their antifungal, biochemical and physiological features. Turk. J. Biol. 27:247-253
14	Papavizas, G. C. 1982. Survival of Trichoderma harzianum in soil and in pea and bean Rhizospheres. Phytopathology 72:121-125 DOI
15	Samuels, G. J. 1996. Tricoderma: a review of biology and systematics of the genus. Mycol. Res. 100:923-935 DOI ScienceOn
16	Went, F. A. F. C. 1896. Notes on sugarcane diseases. Ann. Bot. 10:583-600
17	Burns, J. R. and Benson, D. M. 2000. Biocontrol of damping-off of Catharanthus roseus caused by Pythium ultimum with Trichoderma virens and binucleate Rhizoctonia fungi. Plant Dis. 84:644-648 DOI ScienceOn
18	Rahman, M. A. 2009. Screening of Trichoderma spp. and their efficacy as a bio conversion agent of municipal solid waste through appropriate technique of solid state fermentation. Ph.D. Thesis. University of Rajshahi, Rajshahi, Bangladesh
19	Kuo, T. T., Chien, M. M. and Li, H. W. 1969. Ethyl acetate produced by Ceratocystis paradoxa and C. adiposum and its role in inhibition of germination of sugarcane buds. Can. J. Bot. 47:1459-1463 DOI
20	Skidmore, A. M. and Dickinson, C. H. 1976. Colony interactions and hyphal interference between Septoria Nodorum and phylloplane fungi. Trans. Brit. Mycol. Soc. 66:57-64 DOI
21	Hjeljord, L. G., Stensvand, A. and Tronsmo, A. 2001. Antagonism of nutrient-activated conidia of Trichoderma harzianum (atroviridae) P1 against Botrytis cinerea. Phytopathology 91:1172-1180 DOI ScienceOn
22	Anonymous. 2000. Investigation of varietal resistance/susceptibility against pineapple disease. BSRI Annual Report (1998-99), pp. 86. Bangladesh Sugarcane Research Institute, Pabna
23	Anonymous. 1999. BSRI Annual Report (1997), pp. 89. Bangladesh Sugarcane Research Institute, Pabna
24	Dennis, C. and Webster, J. 1971. Antagonistic properties of species-groups of Trichoderma. I. Production of non-volatile antibiotics. Trans. Brit. Mycol. Soc. 57:25-39 DOI
25	Henis, Y., Adams, P. B., Lewis, J. A. and Papavizas, G. C. 1983. Penetration of sclerotia of Sclerotium rolfsii by Trichoderma spp. Phytopathology 73:1043-1046 DOI

3	(2016) World Journal of Microbiology and Biotechnology Molecular phylogenetics and anti-Pythium activity of endophytes from rhizomes of wild ginger congener, Zingiber zerumbet Smith / 32 (3)
4	(2017) Asian Journal of Plant Pathology Screening and Molecular Characterization of Salt Tolerant Bio-control Bacterial Isolates from Casuarina equisetifolia Rhizosphere Soil / 11 (4) , 156
1664-302X	(2017) Frontiers in Microbiology Non-edible Oil Cakes as a Novel Substrate for DPA Production and Augmenting Biocontrol Activity of Paecilomyces variotii / 8 (1664-302X)
2250-1754	(2019) National Academy Science Letters In Vitro Antagonism of Trichoderma Isolates Against Curvularia andropogonis Causing Leaf Blight of Java Citronella / (2250-1754)