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http://dx.doi.org/10.4489/MYCO.2009.37.1.031

Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol-3-acetate Isolated from Calotropis gigantea (Linn.) Flower  

Habib, M. Rowshanul (Department of Biochemistry and Molecular Biology, Rajshahi University)
Karim, M. Rezaul (Department of Biochemistry and Molecular Biology, Rajshahi University)
Publication Information
Mycobiology / v.37, no.1, 2009 , pp. 31-36 More about this Journal
Abstract
A phytochemical study on the flower of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of $16{\sim}128{\mu}g/ml$. The cytotoxicity ($LC_{50}$) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 ${\mu}g/ml$ for ethyl acetate, 9.19 ${\mu}g/ml$ for compound 1 and 15.55 ${\mu}g/ml$ for compound 2.
Keywords
Anhydrosophoradiol-3-acetate; Antimicrobial; Calotropis gigantea and Di-(2-ethylhexyl) phthalate;
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