[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4489/MYCO.2005.33.2.121

Immobilization of Keratinase from Aspergillus flavus K-03 for Degradation of Feather Keratin

Kim, Jeong-Dong (Department of Life Science, Hanyang University)

Publication Information

Mycobiology / v.33, no.2, 2005 , pp. 121-123 More about this Journal

Abstract

Extracellular keratinase isolated from Aspergillus flavus K-03 was immobilized on calcium alginate. The properties and reaction activities of free and immobilized keratinase with calcium alginate were characterized. The immobilized keratinase showed proteolytic activity against soluble azo-casein and azo-keratin, and insoluble feather keratin. Heat stability and pH tolerance of keratinase were greatly enhanced by immobilization. It also displayed a higher level of heat stability and an increased tolerance toward alkaline pHs compared with free keratinase. During the durability test at $40^{\circ}C$ , 48% of the original enzyme activity of the immobilized keratinase was remained after 7 days of incubation. The immobilized keratinase exhibited better stability, thus increasing its potential for use in industrial application.

Keywords

Durability; Heat stability; Immobilization; Keratinase; pH tolerance;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)

Reference
Cited By KSCI

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