Browse > Article
http://dx.doi.org/10.4489/MYCO.2004.32.3.134

In vitro Effects of Plant Extracts, and Phytohormones on Mycelial Growth of Anthracnose Fungi  

Alam, Shahidul (Department of Botany, University of Rajshahi)
Han, Kee-Don (Department of Biology, Dongguk University)
Lee, Jae-Min (Department of Biology, Dongguk University)
Hur, Hyeon (Department of Biology, Dongguk University)
Shim, Jae-Ouk (Department of Biology, Dongguk University)
Chang, Kwang-Choon (Department of Biology, Dongguk University)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Lee, Min-Woong (Department of Biology, Dongguk University)
Publication Information
Mycobiology / v.32, no.3, 2004 , pp. 134-138 More about this Journal
Abstract
Water extracts of six plants, such as Allium sativum, A. cepa, Zingiber officinale, Platycodon grandiflorum, Oenanthe javanica, and Capsella brusapastoris, were tested in vitro for inhibitory activity against mycelial growth of anthracnose fungi, Colletotrichum gloeosporioides, C. dematium, and C. coccodes. Among the plant extracts, an Allium sativum extract has good inhibitory effects in all the fungi. Four phytohormones namely, IAA(indole-3-acetic acid), NAA(a-Naphthyl acetic acid), 2,4-D(2,4-Dichloro phenoxy acetic acid) and BAP(Benzyl adenine purine) were used to find out the role over mycelial growth of these fungi. All the concentrations of BAP have good inhibitory effect against mycelial growth of these fungi than that of other tested plant hormones.
Keywords
Anthracnose pathogen; Phytohormones; Plant extracts;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Islam, M. R., Alam, S. A., Rahman, M. Z., Chowdhury, S. P., Begum, M. E, Akhter, N., Alam, M. S., Han, K D. and Lee, M. W 2003. Effects of plant extracts on conidial germination, mycelial growth and sporulation of fungi isolated from poultry feed. Mycobiology 31: 221-225
2 Khair, A., Ara, I., Joarder, G. K and Begum, E 1995. Effect of clove oil and citral on toxin producing fungal flora of poultry feed. Bangladesh J. Sci. Indust. Res. 30(2-3): 191-195
3 Park, K S. and Kim, C. H. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Kor. J. Pl. Pathol. 8: 61-69
4 Powell, C. R. and Ko, W. H. 1986. Screening of antagonistic plants for control of Pytophthlota plamivora in soil. Ann. Phytopathol. Soc. Japan 52: 817-824   DOI
5 Singh, H. B. and Singh, U. P. 1981. Effect of volatility of some plants extract on Erysiphe. Indian J. Pl. Pathol. 10: 66-67
6 Yoshida, S., Kasuga, S., Hayashi, N., Ushiroguchi, T., Matsuura, H. and Nakagawa, S. 1987. Antifungal activity of ajoene derived form garlic. Appl. Environ. Microbiol. 53: 615-617
7 Yu, S. H., Park, J. S., Oh, I. S., Wu, I. S. and Mathur, S. B. 1987. Colletotrichum coccodes found in seeds of Capsicum annum and pathogenicity to Solanaceae plants. Kor. J. Mycol. 15: 183-186
8 AI-Masri, M. I., Ali-Shtayeh, M. S., Elad, Y, Sharon, A., Tudzynski, P. and Barakat, R. 2002. Effect of plant growth regulators on white mould (Sclerotinia sclerotiorumi on bean and cucumber. J. Phytopathology 150: 481-487   DOI   ScienceOn
9 Alam, S, Alam, M. S. and Mahal, E 1999. Growth inhibition (in vitro) of chilli fruit rot pathogen Alternaria tenuis. J. Asiat. Soc. Bangladesh, Sci. 25: 211-216
10 Alam, S, Banu, M. S., Ali, M. F., Akhter, N., Islam, M. R. and Alam, M. S. 2002. In vitro inhibition of conidial germination of Colletotrichum gloeosporioides Penz. by fungicides, plant extracts and phytohormones, Pakistan J. Biol. Sci. 5: 303-306
11 Brown, W. 1923. Experiments on the growth of fungi on culture media. Ann. Bot. 37: 105-129
12 Bunkina (Mme), I. A. 1960. Results of the study of diseases of ginseng. Analysis of ginseng seed fungal infection and preswing treatment. Mater. Izuch. Zhen shenya Limonnika 131-162, 163-170. (In Russian Rev. Appl. Mycol. 40: 120)
13 Dubey, R. C. 1991. Fungicidal effect of essential oils of three higher plants on sclerotia of Macrophomina phaseolina. Indian Phytopath, 44: 241-243
14 Edel'Shtein, L. I. 1960. Materials for the study of diseases of ginseng in the Primorskii. Mater. Izuch. Zhen shenya Limonnika 131-162, 163-170. (In Russian Rev. Appl. Mycol. 40: 120)
15 Grayer, R. J. and Harborne, J. B. 1994. A survey of antifungal compounds from higher plants, 1982-1993. Phytochemistry 37: 19-42   DOI   ScienceOn
16 Melinda, J. and Stevenson, W. R. 1991. A leaf disk assay for detection resistance to early blight caused by Alternaria solani in juvenile potato plants. Plant Dis. 75: 385-389   DOI
17 Hong, J. K. and Hwang, B. K. 1998. Influence of inoculum density, wetness duration, plant age, inoculation method, and cultivar resistance on infection of pepper plants by Collctotrichum coccodes. PI. Dis. 82: 1079-1083   DOI   ScienceOn
18 Kim, D. K, Shim, C. K, Bae, D. W, Kawk, Y. S., Yang, M. S. and Kim, H. K 2002. Identification and biological characteristics of an antifungal compound extracted from cocklebur (Xanthium strumarium) against Phytophthora drechsleri. Plant Pathol. J. 18: 288-292
19 Mahadevan, A. and Sridhar, H. 1982. Methods in Physiological Plant Pathology. $2^{nd}$Ed. Sivakami Publications, Madras. pp. 316
20 Miah, M. A. T., Ahmed, H. U., Sharma, N. R., Ali, A. and Miah, S. A. 1990. Antifungal activity of some plant extracts. Bangladesh J. Bot. 19(1): 5-10
21 Michniewicz, M. and Rozej, B. 1987. Further studies on the role of auxin on the growth and development of Fusarium culmorum (W.G.Sm) Sacc. Acta Phyisol. Plant. 9: 219-227
22 Michniewicz, M. and Rozej, B. 1988 Is the gibberellin-limiting factor for the growth and development of Fusarium culmorum? Acta Physiol. Plant 10: 227-236
23 Mishra, A. K and Dubey, N. K 1994. Evaluaton of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Appl. Environ. Microbiol. 60: 1101-1105
24 Nakamura, T., Kawanabe, Y., Takiyama, E., Takahashi, N. and Murayama, T. 1978. Effect of auxin and gibberellin on conidial germination in Neurospora crassa. Plant Cell Physiol. 19: 705-709
25 Nakata, K and Takimoto, S. 1922. Studies on ginseng diseases. Chosen (Korea) Model Exper. Station Report. 5: 25-30
26 Singh, U. P., Pandey, V. N., Wagner, K. G. and Singh, K P. 1990. Antifungal activity of ajoene, a constituent of garlic (Allium sativum). Can. J. Bot. 68(6): 1354 1356
27 Oh, I. S., In, M. S., Woo, I. S., Lee, S. K and Yu, S. H. 1988. Anthracnose of pepper seedling caused by Colletotrichum coccodes (Wallr.) Hughes. Kor.J. Mycol. 16: 151-156
28 Alam, S., Akhter, N., Begum, M. E, Banu, M. S., Islam, M. R., Chowdhury, A. N. and Alam, M. S. 2002. Antifungal activities (in vitro) of some plant extracts and smoke on four fungal pathogens of different hosts. Pakistan J. Biol. Sci. 5: 307-309   DOI
29 Singh, P. P., Barsa, A. S. and Pannu, P. S. 1997. Abscisic acid is a potent inhibitor of growth and sporidial formation in Nevosia indica cultures: dual mode of action via loss of polyamine and cellular turgidity. Phytoparasitica 25: 111-116   DOI   ScienceOn
30 Singh, Y., Tripathi, R. D. Tripathi, N. N. and Dixit, S. N. 1983. The isolation and properties of fungitoxic principle from Zingiber officinale. Indian J. Plant Path. 12: 89-96
31 Snedecor, G. Wand Cochran, W. G. 1980. Statistical methods. 7th ed. Pp: 507. Iowa State Univ. Press, Ames, Iowa U.S.A
32 TeBeest, D.O., Correll, J. C. and Weidemann, G. J. 1997. The Mycota. Edited by K. Esser and P. A. Lemke. Plant Relationship Part B. Springer. pp. 157-168
33 Templeton, C. E. 1992. Use of Colletotrichum strains as mycoherbicides. In; Bailey, J. A. and Jeger, M. J. (eds.) Colletotrichum: Biology, Pathology and Control. CAB International, Wallingford. pp. 358-380
34 Tewari, S. N. 1995. Ocimum sanctum L., a botanical fungicide for rice blast control. Trop. Sci. 35: 263-276
35 Tomita, K, Murayama, T. and Nakamura, T. 1984. Effects of auxin and gibberellin on elongation of young hyphae in Neurospora crassa. Plant Cell Physiol. 25: 355-358
36 Whetzel, H. H. and Rosenbaum, J. 1912. The diseases of ginseng and their control. U. S. Dept. Agr., Bur. Plant Ind. Bull. 250