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http://dx.doi.org/10.4489/MYCO.2004.32.1.006

Molecular Marker Related to Fruitbody Color of Flammulina velutipes  

Kong, Won-Sik (Applied Microbiology Division, National Institute of Agricultural Science & Technology, RDA)
You, Chang-Hyun (Applied Microbiology Division, National Institute of Agricultural Science & Technology, RDA)
Yoo, Young-Bok (Applied Microbiology Division, National Institute of Agricultural Science & Technology, RDA)
Kim, Gyu-Hyun (Department of Biotechnology, Yonam College of Agriculture)
Kim, Kwang-Ho (Department of Agronomy, Konkuk Univ.)
Publication Information
Mycobiology / v.32, no.1, 2004 , pp. 6-10 More about this Journal
Abstract
White and brown strains of Flammulina velutipes were inter-crossed. All $F_1$ showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.
Keywords
Backcross; Bulked segregant analysis; Flammulina velutipes; Molecular marker;
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