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Isolation and Identification of Newly Isolated Antagonistic Streptomyces sp. Strain AP19-2 Producing Chromomycins  

Wu, Xue-Chang (Institute of Microbiology, College of Life Sciences, Zhejiang University)
Chen, Wei-Feng (Institute of Microbiology, College of Life Sciences, Zhejiang University)
Qian, Chao-Dong (Institute of Microbiology, College of Life Sciences, Zhejiang University)
Li, Ou (Institute of Microbiology, College of Life Sciences, Zhejiang University)
Li, Ping (Institute of Microbiology, College of Life Sciences, Zhejiang University)
Wen, Yan-Ping (Institute of Microbiology, College of Life Sciences, Zhejiang University)
Publication Information
Journal of Microbiology / v.45, no.6, 2007 , pp. 499-504 More about this Journal
Abstract
A new antagonistic strain of actinomycete, designated AP19-2, was isolated from the feces of giant pandas inhabiting the Foping National Nature Reserve in China. Cultural characteristic studies strongly suggested that this strain is a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene of strain AP19-2 evidenced profound similarity (97-99 %) with other Streptomyces strains. Two pure active molecules were isolated from a fermentation broth of Streptomyces sp. strain AP19-2 via extraction, concentration, silica gel G column chromatography, and HPLC. The chemical structures of the two related compounds (referred to as chromomycin $A_2$ and chromomycin $A_3$) were established on the basis of their Infrared spectra (IR), High Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), and Nuclear Magnetic Resonance (NMR) data, and by comparison with published data.
Keywords
antagonistic; Streptomyces sp. AP19-2; chromomycin;
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1 Buchanan, R.E. and N.E. Gibbons. 1974. Bergey's Manual of Determinative Bacteriology, 8th ed. The Williams & Wilkins Company, Baltimore, USA
2 Lazzarini, A., L. Cavaletti, G. Toppo, and F. Marinelli. 2000. Rare genera of actinomycetes as potential producers of new antibiotics. Antonie Van Leeuwenhoek 78, 399-405   DOI   ScienceOn
3 Lombo, F., N. Menendez, J.A. Salas, and C. Mendez. 2006. The aureolic acid family of antitumor compounds: structure, mode of action, biosynthesis, and novel derivatives. Appl. Microbiol. Biotechnol. 73, 1-14   DOI
4 Saddler, G.S., P. Tavecchia, S. Locuiro, M. Zanol, L. Colombo, and E. Selva. 1991. Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J. Microbiol. Methods 14, 185-191   DOI   ScienceOn
5 Yue, M., G.D. Dang, and L.J. Yong. 1999. The basic features of vegetation of foping nature conservation in shanxi province. J. Wuhan Botanical Res. (In Chinese) 17, 22-28
6 Staneck, J.L. and G.D. Roberts. 1974. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28, 226-231   PUBMED
7 Edwards, U., T. Rogall, H. Bocker, M. Emde, and E.C. Bottger. 1989. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal DNA. Nucleic Acids Res. 17, 7843-7853   DOI
8 Skehan, P., R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, J.T. Warren, H. Bokesch, S. Kenney, and M.R. Boyd. 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. J. Nat. Cancer Inst. 82, 1107-1112   DOI
9 Weber, D., O. Sterner, T. Anke, S. Gorzalczancy, V. Martino, and C. Acevedo. 2004. Phomol, a new antiinflammatory metabolite from an endophyte of the medicinal plant Erythrina crista-galli. J. Antibiot. 57, 559-563   DOI   ScienceOn
10 Menendez, N., M. Nur-e-Alam, C. Fischer, A.F. Brana, J.A. Salas, J. Rohr, and C. Mendez. 2006. Deoxysugar transfer during chromomycin A3 biosynthesis in Streptomyces griseus subsp. griseus: New derivatives with antitumor activity. Appl. Environ. Microbiol. 72, 167-177   DOI   ScienceOn
11 Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425   PUBMED
12 Demain, A.L. 1999. Pharmaceutically active secondary metabolites of microorganisms. Appl. Microbiol. Biotechnol. 52, 455-463   DOI
13 Mukku, V.J., M. Speitling, H. Laatsch, and E. Helmke. 2000. New butenolides from two marine streptomycetes. J. Nat. Prod. 63, 1570-1572   DOI   ScienceOn
14 Miyamoto, M., Y. Kawamtsu, K. Kawashima, M. Shinohara, K. Tanaka, S. Tatsuoka, and K. Nakanishi. 1967. Chromomycin $A_2,\;A_3,\;and\;A_4$. Tetrahedron 23, 421-437   DOI   ScienceOn
15 Amann, R.I., W. Ludwig, and K.H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59, 143-169   PUBMED
16 Li, J.X. and J.M. Zhao. 2005. Synthetically evaluation on foping national nature conservation. J. Northwest Forestry Univ. (In Chinese) 20, 165-169
17 Shirling, E.B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. Inst. J. Syst. Bacteriol. 16, 313-340   DOI
18 Weisburg, W.G., M.S. Barns, A.D. Pelletier, and J.D. Lane. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697-703   DOI   PUBMED
19 Chen, Y.G., Y. Jiang, X.L. Cui, W.J. Li, S.K. Tan, M.L. Wen, C.L. Jiang, and L.H. Xu. 2006. Isolation and phylogenetic analysis of one moderately halophile YIM 80186 with strong anticancer activity. J. Yunnan Univ. (In Chinese) 28, 444-449
20 Yoshimura, Y., M. Koenuma, K. Matsumoto, K. Tori, and Y. Terui. 1988. NMR studies of chromomycins, olivomycins, and their derivatives. J. Antibiot. 41, 53-67   DOI   PUBMED