Browse > Article

A Comparison of Adult and Pediatric Methicillin-Resistant Staphylococcus aureus Isolates Collected from Patients at a University Hospital in Korea  

Park, Jin-Yeol (Department of Microbiology, Kyungpook National University, School of Medicine)
Jin, Jong-Sook (Department of Microbiology, Kyungpook National University, School of Medicine)
Kang, Hee-Young (Department of Microbiology, Kyungpook National University, School of Medicine)
Jeong, Eun-Hee (Department of Pediatrucs, College of Medicine Dankook University)
Lee, Je-Chul (Department of Microbiology, Kyungpook National University, School of Medicine)
Lee, Yoo-Chul (Department of Microbiology, Kyungpook National University, School of Medicine)
Seol, Sung-Yong (Department of Microbiology, Kyungpook National University, School of Medicine)
Cho, Dong-Taek (Department of Microbiology, Kyungpook National University, School of Medicine)
Kim, Jung-Min (Department of Microbiology, Kyungpook National University, School of Medicine)
Publication Information
Journal of Microbiology / v.45, no.5, 2007 , pp. 447-452 More about this Journal
Abstract
In this study, we compared the phenotypic and genotypic characteristics of 138 MRSA isolates obtained from adult and pediatric patients (adult, 50; children, 88). The resistance rates against gentamicin, clindamycin, and ciprofloxacin were much higher in the adult MRSA isolates than in the pediatric MRSA isolates. The ermC gene, which is responsible for inducible clindamycin resistance, was detected in 52(59.1%) of the 88 pediatric MRSA isolates but in only 5(10.0%) of the 50 adult MRSA isolates. MRSA isolates of clonal type ST5 with an integration of SCCmec type II/II variants was the most predominant clone among the adult isolates, while clonal type ST72 with an integration of SCCmec IV/IVA was the most predominant clone among the pediatric MRSA isolates. Staphylococcal enterotoxin A and toxic shock syndrome toxin-1 were prevalent among the adult MRSA isolates but not among the pediatric MRSA isolates. The results of this study demonstrated remarkable differences between adult and pediatric MRSA isolates in terms of their antimicrobial susceptibility profiles, SCCmec type, multilocus sequence type, staphylococcal toxin genes, and erythromycin resistance genes.
Keywords
MRSA; MLST; SCCmec type; staphylococcal toxin; erythromycin resistance gene;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
연도 인용수 순위
1 Schmitz, F.J., R. Sadurski, A. Kray, M. Boos, R. Geisel, K. Köhrer, J. Verhoef, and A.C. Fluit. 2000. Prevalence of macrolide-resistance genes in Staphylococcus aureus and Enterococcus faecium isolates from 24 European university hospitals. J. Antimicrob. Chemother. 45, 891-894   DOI   ScienceOn
2 Eady, E.D. and J.H. Cove. 2003. Staphylococcal resistance revisited: community-acquired methicillin resistant Staphylococus aureusan emerging problem for the management of skin and soft tissue infections. Curr. Opin. Infect. Dis. 16, 103-124   DOI   PUBMED   ScienceOn
3 Enright, M.C., N.P. Day, C.E. Davies, S.F. Peacock, and B.G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin- resistant and methicilli-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38, 1008-1015   PUBMED
4 Ko, K.S., Y.S. Kim, J. Song, J.S. Yeom, H. Lee, S.I. Jung, D.R. Jeong, S.W. Kim, H.H. Chang, H.K. Ki, C.S. Moon, W.S. Oh, K.R. Peck, and N.Y. Lee. 2005a. Genotypic diversity of methicillin- resistant Staphylococcus aureus isolates in Korean hospitals. Antimicrob. Agents Chemother. 49, 3583-3585   DOI   ScienceOn
5 Ko, K.S., J. Lee, J.Y. Suh, W.S. Oh, K.R. Peck, N.Y. Lee, and J. Song. 2005b. Distribution of major genotypes among methicillin- resistant Staphylococcus aureus clones in Asian countries. J. Clin. Microbiol. 43, 421-426   DOI   ScienceOn
6 Lewis, J.S. and J.H. Jorgensen. 2005. Inducible clindamycin resistance in Staphylococci: should clinicians and microbiologists be concerned?. Clin. Infect. Dis. 40, 280-285   DOI   ScienceOn
7 Lina, G., A. Quaglia, M.E. Reverdy, R. Leclercq, F. Vandenesch, and J. Etienne. 1999a. Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among Staphylococci. Antimicrob. Agents Chemother. 43, 1062-1066   PUBMED
8 Diep, B.A., H.A. Carleton, R.F. Chang, G.F. Sensabaugh, and F. Perdreau-Remington. 2006. Roles of 34 virulence genes in the evolution of hospital- and community-associated strains of methicillin- resistant Staphylococcus aureus. J. Infect. Dis. 193, 1495-1503   DOI   ScienceOn
9 Kim, J.S., W. Song, H.S. Kim, H.C. Cho, K.M. Lee, M.S. Choi, and E.C. Kim. 2006. Association between the methicillin resistance of clinical isolates of Staphylococcus aureus, their staphylococcal cassette chromosome mec (SCCmec) subtype classification, and their toxin gene profiles. Diag. Microbiol. Infect. Dis. 56, 289-295   DOI   ScienceOn
10 David, M.Z., S.E. Crawford, S. Boyle-Vavra, M.A. Hostetler, D.C. Kim, and R.S. Daum. 2006. Contrasting pediatric and adult methicillin-resistant Staphylococcus aureus isolates. Emerg. Infect. Dis. 12, 631-637   DOI   PUBMED   ScienceOn
11 NCCLS. 2003. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A8. 8th (ed). National Committee for Clinical Laboratory Standards (presently Clinical and Laboratory Standards Institute), Wayne, PA, USA
12 Becker, K., R. Roth, and G. Peter. 1998. Rapid and specific detection of toxigenic Staphylococcus aureus: Use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene. J. Clin. Microbiol. 36, 2548-2553   PUBMED
13 Weisblum, B. 1985. Inducible resistance to macrolides, lincosamides and streptogramin type B antibiotics, the resistance phenotype, its biological diversity, and structural elements that regulate expression. J. Antimicrob. Chemother. 16, 63-90   DOI   PUBMED
14 Ardic, N., M. Ozyurt, B. Sareyyupoglu, and B. Hazdenaroglu. 2005. Investigation of erythromycin and tetracycline resistance genes in methicillin-resistant staphylococci. Int. J. Antimicrob. Agents 26, 213-218   DOI   ScienceOn
15 Leclercq, R. 2002. Mechanisms of resistance to macrolides and lincosamides: nature of the resistance elements and their clinical implications. Clin. Infect. Dis. 34, 482-492   DOI   ScienceOn
16 Okuma, K., K. Iwakawa, J.D. Turnidge, W.B. Grubb, J.M. Bell, F.G. O'Brien, G.W. Coombs, J.W. Pearman, T.C. Tenover, M. Kapi, C. Tiensasitorn, T. Ito, and K. Hiramatsu. 2002. Dissemination of new methicillin-resistant Staphylococus aureus clones in the community. J. Clin. Microbiol. 40, 4289-4294   DOI
17 Oliveira, D.C. and H. de Lencastre. 2002. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46, 2155-2161   DOI
18 Ma, X.X., T. Ito, C. Tiensasitorn, M. Jamklang, P. Chongtrakool, S. Boyle-Vavra, R.S. Daum, and K. Hiramatsu. 2002. Novel type of staphylococcal cassette chromosome mec identified in community- acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob. Agents Chemother. 46, 1147-1152   DOI
19 Hisata, K., K. Kuwahara-Arai, M. Yamanoto, T. Ito, Y. Nakatomi, L. Cui, T. Baba, M. Terasawa, C. Sotozono, S. Kinoshita, Y. Yamashiro, and K. Hiramatsu. 2005. Dissemination of methicillin- resistant Staphylococci among healthy Japanese children. J. Clin. Microbiol. 43, 3364-3372   DOI   ScienceOn
20 Lina, G., Y. Peimont, F. Godail-mot, M. Bes, M.O. Peter, V. Gauduchon, F. Vandenesch, and J. Etienne. 1999b. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis. 29, 1128-1132   DOI   ScienceOn
21 NCCLS. 2004. Methods for dilution antimicrobial susceptibility testing for bacteria that grow aerobically. Approved standard M7-A6, 7th Ed. National Committee for Clinical Laboratory Standards (presently Clinical and Laboratory Standards Institute), Wayne, PA, USA
22 Ito, T., Y. Katayama, K. Asada, N. Mori, K. Tsutsumimoto, C. Tiensasitorn, and K. Hiramatsu. 2001. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 45, 1323-1336   DOI   ScienceOn
23 Ladhani, S., S. Robbie, R.C. Garratt, D.S. Chapple, C.L. Joannou, and R.W. Evans. 2001. Development and evaluation of detection systems for staphylococcal exfoliative toxin A responsible for scalded-skin syndrome. J. Clin. Microbiol. 39, 2050-2054   DOI   ScienceOn
24 Cha, H.Y., D.C. Moon, C.H. Choi, J.Y. Oh, Y.S. Jeong, Y.C. Lee, S.Y. Seol, D.T. Cho, H.H. Chang, S.W. Kim, and J.C. Lee. 2005. Prevalence of the ST239 clone of methicillin-resistant Staphylococcus aureus and differences in antimicrobial susceptibilities of ST239 and ST5 clones identified in a Korean hospital. J. Clin. Microbiol. 43, 3610-3614   DOI   ScienceOn