Molecular Characterization of Marine Cyanobacteria from the Indian Subcontinent Deduced from Sequence Analysis of the Phycocyanin Operon (cpcB-IGS-cpcA) and 16S-23S ITS Region
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Premanandh, Jagadeesan
(National Facility for Marine Cyanobacteria, Bharathidasan University)
Priya, Balakrishnan (National Facility for Marine Cyanobacteria, Bharathidasan University) Teneva, Ivanka (Department of Botany, Faculty of Biology, University of Plovdiv) Dzhambazov, Balik (Department of Cell and Molecular Biology, Lund University) Prabaharan, Dharmar (National Facility for Marine Cyanobacteria, Bharathidasan University) Uma, Lakshmanan (National Facility for Marine Cyanobacteria, Bharathidasan University) |
1 | Barker, G.L., P.K. Hayes, and S.L. O'Mahony. 1999. A molecular and phenotypic analysis of Nodularia (Cyanobacteria) from the Baltic Sea. J. Phycol. 35, 931-937 DOI |
2 | Barker, G.L., A. Konopka, B.A. Handley, and P.K. Hayes. 2000b. Genetic variation in Aphanizomenon (Cyanobacteria) colonies from the Baltic Sea and North America. J. Phycol. 36, 947-950 DOI ScienceOn |
3 | Boyer, S.L., V.R. Flechtner, and J.R. Johansen. 2001. Is the 16S-23S rRNA internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in cyanobacteria. Mol. Biol. Evol. 18, 1057-1069 DOI ScienceOn |
4 | Crosbie, N.D., M. Pöckl, and T. Weisse. 2003. Dispersal and phylogenetic diversity of nonmarine Picocyanobacteria, inferred from 16S rRNA gene and cpcBA-Intergenic spacer sequence analyses. Appl. Environ. Microbiol. 69, 5716-5721 DOI |
5 | Dyble, J. H.W. Paerl, and B.A. Neilan. 2002. Genetic characterization of Cylindrospermopsis raciborskii (Cyanobacteria) isolates from diverse geographic origins based on nifH and cpcBA-IGS nucleotide sequence analysis. Appl. Environ. Microbiol. 68, 2567-2571 DOI |
6 | Komárek, J. and K. Anagnostidis. 1989. Modern approach to the classification system of cyanophytes. 4: Nostocales. Arch. Hydrobiol. Suppl./Algological Studies 56, 247-345 |
7 | Manen, J.F. and J. Falque. 2002. The cpcB-cpcA locus as a tool for the genetic characterization of the genus Arthrospira (Cyanobacteria): evidence for horizontal transfer. Int. J. System. Evol. Microbiol. 52, 861-867 DOI ScienceOn |
8 | Neilan, B.A., D. Jacobs, and A.E. Goodman. 1995. Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol. 61, 3875-3883 |
9 | Orcutt, K.M., U. Rasmussen, E.A. Webb, J.B. Waterbury, K. Gundersen, and B. Bergman. 2002. Characterization of Trichodesmium spp. by genetic techniques. Appl. Environ. Microbiol. 68, 2236-2245 DOI |
10 | Payne, M.C., J.R. Johansen, and S.L. Boyer. 2001. Taxonomic resolution of Leptolyngbya (Cyanophyta) utilizing the 16S rRNA gene sequence. 55th Annual Meeting of the Phycological Society of America, June 23-28, 2001, Estes Park, CO, USA; J. Phycol. 37, 40 |
11 | Tillett, D., D.L. Parker, and B.A. Neilan. 2001. Detection of toxigenicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus Microcystis: comparison of toxicities with 16S rRNA and phycocyanin operon (phycocyanin intergenic spacer) phylogenies. Appl. Environ. Microbiol. 67, 2810-2818 DOI ScienceOn |
12 | Wu, X., A. Zarka, and S. Boussiba. 2000. A simplified protocol for preparing DNA from filamentous cyanobacteria. Plant. Mol. Biol. Rep. 18, 385-392 DOI |
13 | Barker, G.L., B.A. Handley, P. Vacharapiyasophon, J.R. Stevens, and P.K. Hayes. 2000a. Allele-specific PCR shows that genetic exchange occurs among genetically diverse Nodularia (Cyanobacteria) filaments in the Baltic Sea. Microbiology 146, 2865-2875 DOI |
14 | Young, J.M., 2001. Implications of alternative classifications and horizontal gene transfer for bacterial taxonomy. Int. J. System. Evol. Microbiol. 51, 945-953 DOI ScienceOn |
15 | Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. 1971. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol. Rev. 35, 171-205 |
16 | Felsenstein, J. 2004. PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA |
17 | Lu, W. 1999. Application of molecular biological techniques to the identification of cyanobacteria. Master's thesis, Liverpool John Moores University, England |
18 | Margheri, M.C., R. Piccardi, S. Ventura, C. Viti, and L. Giovannetti. 2003. Genotypic diversity of oscillatoriacean strains belonging to the genera Geitlerinema and Spirulina determined by 16S rDNA restriction analysis. Curr. Microbiol. 46, 359-364 DOI |
19 | Rippka, R., J. Deruelles, J.B. Waterbury, M. Herdman, and R.Y. Stanier. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111, 1-61 DOI |
20 | Saker, M.L., B.A. Neilan, and D.J. Griffiths. 1999. Two morphological forms of Cylindrospermopsis raciborskii (Cyanobacteria) isolated from Solomon Dam, Palm Island, Queensland. J. Phycol. 35, 599-606 DOI |
21 | Lu, W., E.H. Evans, S.M. McColl, and V.A. Saunders. 1997. Identification of cyanobacteria by polymorphisims of PCRamplified ribosomal DNA spacer regions. FEMS Microbiol. Lett. 153, 147-149 |
22 | Salomon, P.S., S. Janson, and E. Granéli. 2003. Molecular identification of bacteria associated with filaments of Nodularia spumigena and their effect on the cyanobacterial growth. Harmful. Algae 2, 261-272 DOI ScienceOn |
23 | Iteman, I., R. Rippka, N. Tandeau De Marsac, and M. Herdman. 2000. Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria. Microbiol. 146, 1275-1286 DOI |
24 | Litvaitis, M.K. 2002. A molecular test of cyanobacterial phylogeny: inferences from constraint analyses. Hydrobiologia 468, 135-145 DOI ScienceOn |
25 | Komarek, J. and K. Anagnostidis. 2005. Susswasserflora von Mitteleuropa Bd.19/2 Cyanoprokaryota, Oscillatoriales, Verlag: Spektrum Akademischer Verlag; Urban and Fischer Bei Els, 759 p |
26 | Prabaharan, D. 1988. Partial characterization of the marine cyanobacterium Phormidium valderianum 30501 for biotechnological purposes. M. Phil Dissertation, Bharathidasan University, Tiruchirapalli, India |
27 | Nübel, U., F. Garcia-Pichel, and G. Muyzer. 1997. PCR primers to amplify 16S rRNA genes from cyanobacteria. Appl. Environ. Microbiol. 63, 3327-3332 |
28 | Castenholz, R.W. and J.B. Waterbury. 1989. Cyanobacteria. In Stanley, J.T., M.P. Bryant, N. Pfenning, and J.H. Holt (Eds.). Bergy's Manual Systematic Bacteriology, Vol. 3. Section 19: Oxygenic photosynthetic bacteria. Williams and Wilkins, Baltimore, USA |
29 | Fox, G.E., J.D. Wisotzkey, and P. Jurtshuk, Jr. 1992. How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int. J. Syst. Bacteriol. 42, 166-170 DOI ScienceOn |
30 | Garcia-Pichel, F., L. Prufert-Bebout, and G. Muyzer. 1996. Phenotypic and phylogenetic analysis show Microcoleus chthonoplastes to be a cosmopolitan cyanobacterium. Appl. Environ. Microbiol., 62, 3284-3291 |
31 | Teneva, I., B. Dzhambazov, R. Mladenov, and K. Schirmer. 2005. Molecular and phylogenetic characterization of Phormidium species (Cyanoprokaryota) using the cpcBGS- cpcA locus. J. Phycol. 41, 188-194 DOI ScienceOn |
32 | Ballot, A., P. Dadheech, and L. Krienitz. 2004. Phylogenetic relationship of Arthrospira, Phormidium, and Spirulina strains from Kenyan and Indian waterbodies. Arch. Hydrobiol. Suppl./Algological Studies 113, 37-56 |
33 | Baurain, D., L. Renquin, S. Grubisic, P. Scheldeman, A. Belay, and A. Wilmotte. 2002. Remarkable conservation of internally transcribed spacer sequences of Arthrospira ('Spirulina') (Cyanophyceae, Cyanobacteria) strains from four continents and of recent and 30-years-old dried samples from Africa. J. Phycol. 38, 384-393 DOI ScienceOn |
34 | Bolch, C.J.S., S.I. Blackburn, B.A. Neilan, and P.M. Grewe. 1996. Genetic characterization of strains of cyanobacteria using PCR-RFLP of the phycocyanin intergenic spacer and flanking regions. J. Phycol. 32, 445-451 DOI ScienceOn |
35 | Ishida, T., A. Yokota, and J. Sugiyama. 1997. Phylogenetic relationships of filamentous cyanobacterial taxa inferred from 16S rDNA sequence divergence. J. Gen. Appl. Microbiol. 43, 237-241 DOI ScienceOn |
36 | Janson, S. and E. Granéli. 2002. Phylogenetic analyses of nitrogen-fixing cyanobacteria from the Baltic Sea reveal sequence anomalies in the phycocyanin operon. Int. J. System. Evol. Microbiol. 52, 1397-1404 DOI ScienceOn |
37 | Li, X. 2000. Phenotypic and genotypic characterization of Nostoc species from 6 different sites in the Mojave desert. Master's thesis, John Carroll University, Cleveland, Ohio |
38 | Scheldeman, P., D. Baurain, R. Bouhy, M. Scott, M. Mühling, B.A. Whitton, A. Belay, and A. Wilmotte. 1999. Arthrospira ('Spirulina') strains from four continents are resolved in only two clusters, based on amplified ribosomal DNA restriction analysis on the internally transcribed spacer. FEMS Microbiol. Lett. 172, 213-222 DOI |
39 | Robertson, B.R., N. Tezuka, and M.M. Watanabe. 2001. Phylogenetic analyses of Synechococcus strains (Cyanobacteria) using sequences of 16S rDNA and part of the phycocyanin operon reveal multiple evolutionary lines and reflect phycobilin content. Int. J. Syst. Evol. Microbiol. 51, 861-871 DOI ScienceOn |
40 | Taton, A., S. Grubusic, E. Brambilla, R.D. Wit, and A. Wilmotte. 2003. Cyanobacterial diversity in natural and artificial microbial mats of lake fryxell (Mc Murdo dry valleys, Antartica): a morphological and molecular approach. Appl. Environ. Microbiol. 69, 5157-5169 DOI |
41 | Otsuka, S., S. Suda, R. Li, S. Matsumoto, and M. Watanabe. 2000. Morphological variability of colonies of Microcystis morphospecies in culture. J. Gen. Appl. Microbiol. 46, 39-50 DOI ScienceOn |
42 | Anagnostidis, K. and J. Komarek. 1985. Modern approach to the classification system of cyanophytes 1-Introduction. Arch. Hydrobiol./ Suppl. 71, Algological. Studies 38 / 39, 291-302 |
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