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Nucleocapsid Amino Acids 211 to 254, in Particular, Tetrad Glutamines, are Essential for the Interaction Between the Nucleocapsid and Membrane Proteins of SARS-Associated Coronavirus  

Fang, Xiaonan (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Ye, Lin-Bai (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Zhang, Yijuan (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Li, Baozong (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Li, Shanshan (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Kong, Lingbao (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Wang, Yuhua (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Zheng, Hong (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Wang, Wei (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Wu, Zhenghui (State Key Laboratory of Virology, College of Life Science, Wuhan University)
Publication Information
Journal of Microbiology / v.44, no.5, 2006 , pp. 577-580 More about this Journal
Abstract
GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.
Keywords
SARS-CoV; membrane protein; nucleocapsid protein; M-N interaction; GST pull-down assay;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
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