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Removal of Contaminating TEM-la $\beta-Lactamase$ Gene from Commercial Taq DNA Polymerase  

Song Jae Seok (Department of Biological Sciences, Myongji University)
Lee Jung Hun (Department of Biological Sciences, Myongji University, Marine Biotechnology Center, Korea Ocean Research & Development Institute)
Lee Jung-Hyun (Marine Biotechnology Center, Korea Ocean Research & Development Institute)
Jeong Byeong Chul (Department of Biological Sciences, Myongji University)
Lee Won-Keun (Department of Biological Sciences, Myongji University)
Lee Sang Hee (Department of Biological Sciences, Myongji University)
Publication Information
Journal of Microbiology / v.44, no.1, 2006 , pp. 126-128 More about this Journal
Abstract
This study confirms that Taq DNA polymerase could be contaminated with the $blaTEM-1_a$ gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.
Keywords
Decontamination; DNase I; Taq DNA polymerse;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
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