Browse > Article

Isolation and Characterization of Bacteria Associated with Two Sand Dune Plant Species, Calystegia soldanella and Elymus mollis  

Park Myung Soo (Korea Research Institute of Bioscience and Biotechnology)
Jung Se Ra (Korea Research Institute of Bioscience and Biotechnology)
Lee Myoung Sook (Korea Research Institute of Bioscience and Biotechnology)
Kim Kyoung Ok (Korea Research Institute of Bioscience and Biotechnology)
Do Jin Ok (Korea Research Institute of Bioscience and Biotechnology)
Lee Kang Hyun (Korea Research Institute of Bioscience and Biotechnology)
Kim Seung Bum (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University)
Bae Kyung Sook (Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Microbiology / v.43, no.3, 2005 , pp. 219-227 More about this Journal
Abstract
Little is known about the bacterial communities associated with the plants inhabiting sand dune ecosystems. In this study, the bacterial populations associated with two major sand dune plant species, Calystegia soldanella (beach morning glory) and Elymus mollis (wild rye), growing along the costal areas in Tae-An, Chungnam Province, were analyzed using a culture-dependent approach. A total of 212 bacteria were isolated from the root and rhizosphere samples of the two plants, and subjected to further analysis. Based on the analysis of the 16S rDNA sequences, all the bacterial isolates were classified into six major phyla of the domain Bacteria. Significant differences were observed between the two plant species, and also between the rhizospheric and root endophytic communities. The isolates from the rhizosphere of the two plant species were assigned to 27 different established genera, and the root endophytic bacteria were assigned to 21. Members of the phylum Gammaproteobacteria, notably the Pseudomonas species, comprised the majority of both the rhizospheric and endophytic bacteria, followed by members of Bacteroidetes and Firmicutes in the rhizosphere and Alphaproteobacteria and Bacteroidetes in the root. A number of isolates were recognized as potentially novel bacterial taxa. Fifteen out of 27 bacterial genera were commonly found in the rhizosphere of both plants, which was comparable to 3 out of 21 common genera in the root, implying the host specificity for endophytic populations. This study of the diversity of culturable rhizospheric and endophytic bacteria has provided the basis for further investigation aimed at the selection of microbes for the facilitation of plant growth.
Keywords
rhizobacteria; endophytic bacteria; sand dune plant; 16S rDNA sequence; Pseudomonas;
Citations & Related Records

Times Cited By Web Of Science : 26  (Related Records In Web of Science)
Times Cited By SCOPUS : 23
연도 인용수 순위
1 Bakker, A.W. and P. Schippers. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Psudomonas spp.-mediated plant growth-stimulation. Soil Biol. Biochem. 19, 451-457   DOI   ScienceOn
2 Cieslinski, G., K.C.J. Van-Rees, A.M. Szmigielska, and P.M. Huang. 1997. Low molecular weight organic acids released from roots of durum wheat and flax into sterile nutrient solutions. J. Plant Nutr. 20, 753-764   DOI   ScienceOn
3 Glick, B.R. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41, 109-117   DOI   ScienceOn
4 Grayston, S.J., D. Vaughan, and D. Jones. 1996. Rhizosphere carbon flow in tree, in comparison with annual plants, the importance of root exudation and its impact on microbial diversity and nutrient availability. Appl. Soil Ecol. 5, 29-56   DOI   ScienceOn
5 Hallmann, J., A. Quadt-Hallmann, W.F. Mahaffee, and J.W. Kloepper 1997. Bacteria endophytes in agricultural crops. Can. J. Microbiol. 43, 895-914   DOI   ScienceOn
6 Lim, J.-S., M.-K. Jung, M.-S. Kim, J.-H. Ahn, and J.-O. Ka. 2004. Genetic and phenotypic diversity of (R/S)-mecoprop [2-(2-methyl-4-chlorophenoxy)propionic acid]-degrading bacterial isolated from soils. J. Microbiol. 42, 87-93.
7 Lucy, M., E. Reed, and B.R. Glick. 2004. Applications of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek. 86, 1-25   DOI   ScienceOn
8 Mantelin, S. and B. Touraine. 2004. Plant growth-promoting bacteria and nitrate availability; impacts on root development and nitrate uptake. J. Exp. Bot. 55, 27-34   DOI   ScienceOn
9 Siciliano, S.D. and J.J. Germida. 1999. Taxonomic diversity of bacteria associated with the roots of field-grown transgenic Brassica napus cv. Quest, compared to the non-transgenic B. napus cv. Excel and B. rapa cv. Parkland. FEMS Microbiol. Ecol. 29, 263-272   DOI   ScienceOn
10 Smith, S.E. and D.J. Read. 1997. Mycorrhizal Symbiosis. Academic Press, San Diego, California, USA
11 Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876-4882
12 Whipps, J.M. 1990. Carbon economy, p. 59-97. In J.M. Lynch (ed.), The Rhizosphere. John Wiley & Sons, Essex, UK
13 Germida, J.J., S.D. Siciliano, J.R. de Freitas, and A.M. Seib. 1998. Diversity of root-associated bacteria associated with fieldgrown canola (Brassica napus L.) and wheat (Triticum aestivum L.). FEMS Microbiol. Ecol. 26, 43-50   DOI   ScienceOn
14 Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph. D. thesis, University of Newcastle, Newcastle upon Tyne, UK
15 Lemanceau, P., T. Corbererand, L. Gardan, X. Latour, G. Laguerre, J-M. Boeufgras, and C. Alabouvette. 1995. Effect of two plant species, flax (Linum usitatissinum) and tomato (Lycopersicon esculentum Mill) on the diversity of soilborne populations of fluorescent Pseudomonas. Appl. Environ. Microbiol. 61, 1004-1012   PUBMED
16 Lilley, A.K., J.C. Fry, M.J. Bailey, and M.J. Day. 1996. Comparison of aerobic heterotrophic taxa isolated from four root domains of mature sugar beet (Beta vulgaris). FEMS Microbiol. Ecol. 21, 231-242   DOI   ScienceOn
17 Jacobson, C.B., J.J. Pasternak, and B.R. Glick. 1994. Partial purification and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the plant growth promoting rhizobacterium Psudomonas putida GR12-2. Can. J. Microbiol. 40, 1019-1025   DOI   ScienceOn
18 Mahaffee, W.F. and J.W. Kloepper. 1997. Temporal changes in the bacterial communities of soil, rhizosphere, and endorhiza associated with field-grown cucumber (Cucumis sativus L.). Microb. Ecol. 34, 210-223   DOI   ScienceOn
19 Van Loon, L.C., P.A. Bakker, and C.M. Pieterse. 1998. Systemic resistance induced by rhizosphere bacteria. Ann. Rev. Phytopathol. 36, 453-483   DOI   ScienceOn
20 Jeon, J.-S., S.-S. Lee, H.-Y. Kim, T.-S. Ahn, and H.-G. Song. 2003. Plant growth promotion in soil by some inoculated microorganisms. J. Microbiol. 41, 271-276
21 Park, H.-D. and J.-O. Ka. 2003. Genetic and phenotypic diversity of dichloroprop-degrading bacteria isolated from soils. J. Microbiol. 41, 7-15
22 Marschner, P., C.H. Yang, R. Lieberei, and D.E. Crowley. 2001. Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biol. Biochem. 33, 1437-1445   DOI   ScienceOn
23 Grayston, S.J., S. Wang, C.D. Campbell, and A.C. Edwards. 1998. Selective influence of plant species on microbial diversity in the rhizosphere. Soil Biol. Biochem. 30, 369-378   DOI   ScienceOn
24 Chanway, C.P., L.M. Nelson, and F.B. Holl. 1988. Cultivar-specific growth promotion of spring wheat (Triticum aestivum L.) by coexistent Bacillus species. Can. J. Microbiol. 34, 925-929   DOI
25 Read, D.J. 1998. Mycorrhizas and nutrient cycling in sand dune ecosystems. Coastal Sand Dunes, vol. 96, p. 89-110. In C.H. Gimmingham, W. Ritchie, B.B. Willetts and A.J. Willis (eds.), Proceedings of Symposium. The Royal Society of Edinburgh, Edinburgh
26 Lane, D.J. 1991. 16S/23S rRNA sequencing, p. 115-175. In E. Stackebrandt and M. Goodfellow (eds.), Nucleic Acid Techniques in Bacterial Systematics. John Weley & Sons, Chichester, UK
27 Whipps, J.M. 2001. Microbial interaction and biocontrol in the rhizosphere. J. Exp. Bot. 52, 487-511   DOI   PUBMED   ScienceOn
28 Marilley, L. and M. Aragno. 1999. Phylogenetic diversity of bacterial communities differing in degree of proximity of Lolium perenne and Trifolium repens roots. Appl. Soil Ecol. 13, 127-136   DOI   ScienceOn
29 Wei, G.J., W. Kloepper, and S. Tuzun. 1996. Induced systemic resistance to cucumber disease and increased plant growth by plant growth-promoting rhizobacteria under field condition. Phytopathology 86, 221-224   DOI
30 Lee, D.-H., S.-A. Noh, and C.-K. Kim. 2000. Development of molecular biological methods to analyze bacterial species diversity in freshwater and soil ecosystems. J. Microbiol. 38, 11-17
31 Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783-791   DOI   ScienceOn
32 Sylvia, D.M. and N.J. Burks. 1988. Selection of a vesicular-arbuscular mycorrhizal fungus for practical inoculation of Uniola paniculata. Mycologia 80, 565-568   DOI
33 Torsvik, V., J. Gokoyr, and F.L. Daae. 1990. High diversity in DNA of soil bacteria. Appl. Environ. Microbiol. 56, 782-787   PUBMED
34 Maloney, P.E., A.H.C. Van Bruggen, and S. Hu. 1997. Bacterial community structure in relation to the carbon environments in lettuce and tomato rhizosphere and in bulk soil. Microb. Ecol. 34, 109-117   DOI   ScienceOn
35 Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16, 111-120   DOI   PUBMED
36 Merbach, W., E. Mirus, G. Knof, R. Remus, S. Ruppel, R. Russow, A. Gransee, and J. Schulze. 1999. Release of carbon and nitrogen compounds by plant roots and their possible ecological importance. J. Plant Nutr. Soil Sci. 162, 373-383   DOI
37 Singh, U.P., B.K. Sarma, and D.P. Singh. 2003. Effect of plant-promoting rhizobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (Cier arietinum). Curr. Microbiol. 46, 131-140   DOI   ScienceOn
38 Dalton, D.A., S. Kramer, N. Azios, S. Fusaro, E. Cahill, and C. Kennedy. 2004. Endophytic nitrogen fixation in dune grasses (Ammophila arenaria and Elymus mollis) from Oregon. FEMS Microbiol. Ecol. 49, 469-479   DOI   ScienceOn
39 Miethling, R., G. Weiland, H. Backhaus, and C.C. Tebbe. 2000. Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L 33. Microb. Ecol. 40, 43-56   DOI   PUBMED