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Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium  

Yoo So Young (Department of Oral Biochemistry, College of Dentistry, Chosun University)
Kim Pyung Sik (Department of Conservative Dentistry, College of Dentistry, Chosun University)
Hwan Ho Keel (Department of Conservative Dentistry, College of Dentistry, Chosun University)
Lim Seong Hoon (Department of Orthodontics, College of Dentistry, Chosun University)
Kim Kwang Won (Department of Orthodontics, College of Dentistry, Chosun University)
Choe Son Jin (Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University)
Min Byung Moo (Department of Oral Biochemistry, College of Dentistry, Seoul National University)
Kook Joong Ki (Department of Oral Biochemistry, College of Dentistry, Seoul National University)
Publication Information
Journal of Microbiology / v.43, no.2, 2005 , pp. 204-208 More about this Journal
Abstract
The objective of this study was to both isolate and identify non-mutans streptococci organisms (non­MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies). These results strongly suggest that a new selective medium will be required for the reliable isolation of mutans streptococci.
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