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Isolation and Characterization of $\alpha$-Glucosidase Inhibitor from the Fungus Ganoderma lucidum  

Kim, Shin-Duk (Department of Biological Engineering, Seokyeong University)
Nho, Hong Joon (Department of Biological Engineering, Seokyeong University)
Publication Information
Journal of Microbiology / v.42, no.3, 2004 , pp. 223-227 More about this Journal
Abstract
An ${\alpha}$-glucosidase inhibitor, SKG-3, was isolated from the fruiting bodies of Ganoderma lucidum and its physico-chemical properties were characterized. It was a highly specific and effective reversible inhibitor of ${\alpha}$-glucosidase. It showed very potent inhibitory activity against a-glucosidase with an IC$\sub$50/ value of 4.6$\mu\textrm{g}$/$m\ell$, but no activity for any other glycosidases tested. Enzyme activity could be recovered upon dialysis, thus providing evidence for the reversibility of the inhibition. A Lineweaver-Burk plot indicated that the SKG-3 inhibition of ${\alpha}$-glucosidase was competitive.
Keywords
${\alpha}$-glucosidase inhibitor; Ganoderma lucidum; reversible; competitive inhibitor;
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1 Asano, N. 2003. Glycosidase inhibitors: Update and perspectives on practical use. Glycobiology 13, 93R-104R.
2 Fischer, P., M. Collin, G. Karlsson, W. James, T. Butters, S. Davis, S. Gordon, R. Dwek, and F. Platt. 1995. The $\alpha$-glucosidase inhibitor, N-butyldeoxynojirimycin inhibits human immunodeficiency virus entry at the level of post-CD4 binding. J. Virol. 69, 5791-5797.
3 Kornfeld, R. and S. Kornfeld. 1985. Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem. 54, 631-664
4 O'Dea, K. and J. Turton. 1985. Optimum effectiveness of intestinal $\alpha$-glucosidase inhibitors. Am J Clin Nutr. 41, 511-516.
5 Sunkara, P., T. Bowlin, P. Liu, and A. Sjoerdsma. 1987. Antiretroviral activity of castanospermine and deoxynojirimycin, specific inhibitors of glycoprotein processing. Biochem. Biophys. Res. Commun. 148, 206-210
6 Pan, Y., J. Ghidoni, and D. Elbein. 1993. The effects of castanospermine and swainsonine on the activity and synthesis of intestinal sucrase. Arch. Biochem. Biophys. 303, 134-144
7 Cenci di Bello, I., G. Fleet, K. Namgoong, K. Tadano, and B. Winchester. 1989. Structure-activity relationship of swainsonine: inhibition of human $\alpha$-mannosidase by swainsonine analogues. Biochem. J. 259, 855-861.
8 Molyneux, R., R. McKenzie, B. O’Sullivan, and A. Elbein. 1995. Identification of the glycosidase inhibitors swainsonine and calystegine B2 in weir vine and correlation with toxicity. J. Nat. Prod. 58, 878-886
9 Molyneux, R., R. Nash, and N. Asano. 1996. The chemistry and biological activity of calystegines and related nortropane alkaloids, p. 303-343. In S. Pelletier (ed.), Alkaloids: Chemical and Biological Perspectives, 11. Elsevier Science Ltd, Oxford, UK
10 Jacob, G.S. 1995. Glycosylation inhibitors in biology and medicine. Curr. Opin. Struct. Biol. 5, 605-611
11 Min, B.S., J.J. Gao, N. Nakamura, and M. Hattori. 2000. Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against meth-A and LLC tumor cells. Chem. Pharm. Bull. 48, 1026-1033
12 Watson, A., G. Fleet, N. Asano, R. Molyneux, and R. Nash. 2001. Polyhydroxylated alkaloids-natural occurrence and therapeutic applications. Phytochemistry 56, 265-295
13 Murai, A., K. Iwamura, M. Takada, K. Ogawa, T. Usui, and J. Okumura. 2002. Control of postprandial hyperglycaemia by galactosyl maltobionolactone and its novel anti-amylase effect in mice. Life Sci. 71, 1405-1415
14 Saul, R., J. Chambers, R. Molyneux, and A. Elbein. 1983. Castanospemine, a tetrahydroxylated alkaloid that inhibits $\beta$-glucosidase and $\beta$-glucocerebrosidase. Arch. Biochem. Biophys. 221, 593-597.
15 Hikino, H., C. Konno, Y. Mirin, and T. Hayashi. 1985. Isolation and hypoglycemic activity of Ganoderans A and B, glycans of Ganoderma lucidum fruit bodies. Pla. Med. 51, 339-340