Browse > Article

Glutathione Content and the Activities of Glutathione-Synthesizing Enzymes in Fission Yeast are Modulated by Oxidative Stress  

Lee, Yuk-Young (Division of Life Sciences, Kangwon National University)
Kim, Su-Jung (Division of Life Sciences, Kangwon National University)
Park, Eun-Hee (College of Pharmacy, Sookmyung Womens University)
Lim, Chang-Jin (Division of Life Sciences, Kangwon National University)
Publication Information
Journal of Microbiology / v.41, no.3, 2003 , pp. 248-251 More about this Journal
Abstract
Glutathione (GSH) is an important factor in determining tolerance against oxidative stress in living organisms. It is synthesized in two sequential reactions catalyzed by ${\gamma}$-glutamylcysteine synthetase (GCS) and glutathione synthetase (GS) in the presence of ATP. In this work, the effects of three different oxidative stresses were examined on GSH content and GSH-related enzyme activities in the fission yeast Schizosaccharomyces pombe. GSH content in S. pombe was significantly enhanced by treatment with hydrogen peroxide, ${\beta}$-naphthoflavone (BNF) and tert-butylhydroquinone (BHQ). Simultaneously, they greatly induced GCS and GS activity. However, they did not have any effects on glutathione reductase activity. These results suggest that GCS and GS activities in S. pombe are up-regulated by oxidative stress.
Keywords
tert-butylhydroquinone; ${\gamma}$-glutamylcysteine synthetase; glutathione; glutathione synthetase; hydrogen peroxide; ${\beta}$-naphthoflavone; Schizosaccharomyces pombe;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
Times Cited By SCOPUS : 9
연도 인용수 순위
1 Induction of glutathione synthetase by 1,10-phenan-throline /
[ Sun,Y. ] / FEBS Letters   DOI   ScienceOn
2 Glutathione metabolism and its role in hepatotoxicity /
[ DeLeve,L.;N.Kaplowitz ] / Pharmacol. Ther.   DOI   ScienceOn
3 Increased transiption of the regulatory subunit of gamma-glutamylcysteine synthetase in rat lung epithelial L2 cells exposed to oxidative stress or glutathione depletion /
[ Tian,L.;M.M.Shin;H.J.Forman ] / Arch. Biochem. Biophys.   DOI
4 Up-regulation of glutamate-cysteine ligase gene expression by butylated hydroxytoluene is mediated by transcription factor AP-1 /
[ Tu,Z.;M.W.Anders ] / Biochem. Biophys. Res. Commun.   DOI   ScienceOn
5 Effects of redox cycling compounds on glutathione content and activity of glutathione-related enzymes in rainbow trout liver /
[ Stephensen,E.;J.Sturve;L.Forlin ] / Comp. Biochem. Physiol. Part C
6 Enhanced expression of pulmonary γ-glutamylcysteine synthetase heavy subunit in rats exposed to cadmium aerosols /
[ Shukla,G.S.;J.F.Chiu;B.A.Hart ] / Toxicol. Appl. Pharmacol.   DOI
7 Regulation of antioxidant enzymes gene expression in the yeast Saccharomyces cerevisiae during stationary phase /
[ Cyrne,L.;L.Martins;L.Fernandes;H.S.Marinho ] / Free Radic. Biol. Med.   DOI   ScienceOn
8 Effect of thioacetamide on the hepatic expression of γ-glutamylcysteine synthetase subunits in the rat /
[ Lu,S.C.;Z.Z.Huang;H.Yang;H.Tsukamoto ] / Toxicol. Appl. Pharmacol.   DOI
9 Differential responses from seven mammalian cell lines to the treatments of detoxifying enzyme inducers /
[ Jiang,Z.Q.;C.Chen;B.Yang;V.Hebbar;A.N.T.Kong ] / Life Sci.   DOI   ScienceOn
10 Thioredoxin peroxidase is required for the transcriptional response to oxidative stress in budding yeast /
[ Ross,S.J.;V.J.Findlay;P.Malakasi;B.A.Morgan ] / Mol. Biol. Cell   DOI
11 Cadmium-induced elevations in the gene expression of the regulatory subunit of γ-glutamylcysteine synthetase in rat lung and alveolar epithelial cells /
[ Shukla,G.S.;J.F.Chiu;B.A.Hart ] / Toxicol.   DOI
12 Glutathione disulfide (GSSG) efflux from cells and tissues /
[ Sies,H.;T.P.Akerboom ] / Methods Enzymol.   DOI
13 Metabolism and function of glutathione /
[ Meister,A.;D.Dolphin(ed.);R.Poulson(ed.);R.Auramovic(ed.) ] / Glutathione: chemical, biochemical, and medical aspects
14 An electrophile responsive element (EpRE) regulates β-naphthoflavone induction of the human γ-glutamylcysteine synthetase regulatory subunit gene /
[ Moinova,H.R.;R.T.Mulcahy ] / J. Biol. Chem.   DOI   ScienceOn
15 The Yap1p-dependent induction of glutathione synthesis in heat shock response of Saccharomyces cerevisiae /
[ Sugiyama,K.;S.Izawa;Y.Inoue ] / J. Biol. Chem.   DOI   ScienceOn
16 Glutathione reductase /
[ Carlberg,I.;B.Mannervik ] / Methods Enzymol.   DOI
17 Cloning and regulation of Schizosaccharomyces pombe gene encoding ribosomal protein S20 /
[ Lee,Y.J.;K.Kim;E.H.Park;K.S.Ahn;D.Kim;C.J.Lim ] / J. Microbiol.   과학기술학회마을
18 Constitutive and beta-naphthoflavone-induced expression of the human gamma-glutamylcysteine synthetase heavy subunit gene is regulated by a distal antioxidant response element/TRE sequence /
[ Mulcahy,R.T.;M.A.Wartman;H.H.Bailey;J.J.Gipp ] / J. Biol. Chem.   DOI   ScienceOn
19 Glutathione synthetase from rat kidney /
[ Meister,A. ] / Methods Enzymol.   DOI
20 Oxidative stress, age-dependent [correction of age-related] cell damage and antioxidative mechanisms /
[ Gosslau,A.;L.Rensing ] / Z. Gerontol. Geriatr.   DOI   ScienceOn
21 Effects of menadione and hydrogen peroxide on glutathione status in growing Escherichia coli /
[ Smirnova,G.V.;N.G.Muzyka;M.N.Glukhovchenko;U.N.Oktyabrsky ] / Free Radic. Biol. Med.   DOI   ScienceOn
22 Regulation of γ-glutamate-cysteine ligase expression by oxidative stress in the mouse preimplantation embryo /
[ Stover,S.K.;G.A.Gushansky;J.J.Salmen;G.S.Gardiner ] / Toxicol. Appl. Pharmacol.   DOI
23 Tumor necrosis factor increases hepatocellular glutathione by transcriptional regulation of the heavy subunit chain of γ-glutamylcysteine synthetase /
[ Morales,A.;C.Garcia-Ruiz;M.Miranda;M.Mari;A.Colell;E.Ardite;J.C. Fernandez-Checa ] / J. Biol. Chem.   DOI   ScienceOn
24 Bioassay of cadmium using a DNA microassay: genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium /
[ Momose,Y.;H.Iwahashi ] / Environ. Toxicol. Chem.   DOI   ScienceOn
25 Glutathione biosynthesis; γ-Glutamylcysteine synthetase from rat kidney /
[ Seelig,G.F.;A.Meister ] / Methods Enzymol.   DOI
26 Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network /
[ Dormer,U.H.;J.Westwater;N.F.McLaren;N.A.Kent;J.Mellor;D.J.Jamieson ] / J. Biol. Chem.   DOI   ScienceOn
27 Oxidant regulation of the Saccharomyces cerevisiae GSH1 gene /
[ Dormer,U.H.;J.Westwater;D.W.S.Stephen;D.J.Jamieson ] / Biochim. Biophys. Acta   DOI   ScienceOn
28 A short review on the role of glutathione in the response of yeasts to nutritional, environmental, and oxidative stresses /
[ Penninckx,M. ] / Enzyme Microb. Technol.   DOI   ScienceOn
29 Nitric oxide-dependent induction of glutathione synthesis through increased expression of γ-glutamylcysteine synthetase /
[ Moellering,D.;J.McAndrew;R.P.Patel;T.Cornwell;T.Lincoln;X.Cao;J.L.Messina;H.J.Forman;H.Jo;V.M. Darley-Usmar ] / Arch. Biochem. Biophys.   DOI