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Glutathione Content and the Activities of Glutathione-Synthesizing Enzymes in Fission Yeast are Modulated by Oxidative Stress  

Lee, Yuk-Young (Division of Life Sciences, Kangwon National University)
Kim, Su-Jung (Division of Life Sciences, Kangwon National University)
Park, Eun-Hee (College of Pharmacy, Sookmyung Womens University)
Lim, Chang-Jin (Division of Life Sciences, Kangwon National University)
Publication Information
Journal of Microbiology / v.41, no.3, 2003 , pp. 248-251 More about this Journal
Abstract
Glutathione (GSH) is an important factor in determining tolerance against oxidative stress in living organisms. It is synthesized in two sequential reactions catalyzed by ${\gamma}$-glutamylcysteine synthetase (GCS) and glutathione synthetase (GS) in the presence of ATP. In this work, the effects of three different oxidative stresses were examined on GSH content and GSH-related enzyme activities in the fission yeast Schizosaccharomyces pombe. GSH content in S. pombe was significantly enhanced by treatment with hydrogen peroxide, ${\beta}$-naphthoflavone (BNF) and tert-butylhydroquinone (BHQ). Simultaneously, they greatly induced GCS and GS activity. However, they did not have any effects on glutathione reductase activity. These results suggest that GCS and GS activities in S. pombe are up-regulated by oxidative stress.
Keywords
tert-butylhydroquinone; ${\gamma}$-glutamylcysteine synthetase; glutathione; glutathione synthetase; hydrogen peroxide; ${\beta}$-naphthoflavone; Schizosaccharomyces pombe;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
Times Cited By SCOPUS : 9
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