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Stimulation of Tissue Transglutaminase Activity by Clostridium botulinum Neurotoxin Type B

Moon, Yu-Seok (Department of Biological Science, Korea advanced institute of Science and Technology)
Gi-Hyeok (Microbial Toxin Research Institute, Medy-Tox Inc.)
Rhee, Sang-Dal (Department of Biological Science, Korea advanced institute of Science and Technology)
Jung, Hyun-Ho (Microbial Toxin Research Institute, Medy-Tox Inc.,Division of Applied Biological Sciences, Sunmoon University)

Publication Information

Journal of Microbiology / v.41, no.2, 2003 , pp. 161-164 More about this Journal

Abstract

Recombinant light chain of Clostridium botulinum neurotoxin type B stimulated transglutaminase activity in a dose dependent manner, Compared to native toxin, recombinant light chain showed av greater stimulatory effect on transglutaminase activity. Zn-chelating agents, inhibiting the proteolytic activity of the clostridial toxins, did not interfere with this stimulation. These results suggest that the light chain plays a major stimulatory role, which is not due to its metallopeptidase activity, but is possibly due to specific interaction with transglutaminase. More importantly, this report provides a new insight into the intracellular action of C. botulinum neurotoxins.

Keywords

Clostridium botulinum neurotoxin type B; Zn-chelating agent; transglutaminase;

Citations & Related Records

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