Browse > Article

Synthetic Lethal Mutations with spmex67 of Schizosaccharomyces pombe in the Mediation of mRNA Export  

Yoon, Jin-Ho (Department of Biology, Sungshin Women′s University)
Publication Information
Journal of Microbiology / v.41, no.2, 2003 , pp. 115-120 More about this Journal
Abstract
Mex67p/Tap are evolutionally conserved mRNA export factors. To identify mutations in genes that are functionally linked to mex67 with respect to mRNA export, we used a synthetic lethal genetic screen in Schizosaccharomyces pombe. Three synthetic lethal mutants were isolated and mutations in these mutants defined separate complementation groups. These mutants exhibited the accumulation of poly A$\^$+/ RNA in the nucleus, with a decrease in the cytoplasm under synthetically lethal conditions, suggesting that the mutations cause an mRNA nuclear export defect. In addition, the S. pombe genes that were found to be involved in mRNA export did not suppress the synthetic lethality of these mutants. These results indicate that the isolated mutants contain mutations in new genes, which are involved in mRNA export from the nucleus.
Keywords
mex67; synthetic lethality; mRNA export; Schizosaccharomyces pombe;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 A conserved mRNA export machinery coupled to pre-mRNA splicing /
[ Reed,R.;E.Hurt ] / Cell   DOI   ScienceOn
2 A new view of mRNA export: separating the wheat from the chaff /
[ Reed,R.;K.Magni ] / Nat. Cell. Biol.   DOI
3 Transport of proteins and RNAs in and out of the nucleus /
[ Nakielny,S.;G.Dreyfuss ] / Cell   DOI   ScienceOn
4 Npp106p, a Schizosaccharomyces pombe nucleoporin similar to Saccharomyces cerevisiae Nic96p, functionally interacts with Rae1p in mRNA export /
[ Yoon,J.H.;W.A.Whalen;A.Bharathi;R.Shen;R.Dhar ] / Mol. Cell Biol.
5 Protein and RNA export from the nucleus /
[ Lei,E.P.;P.A.Silver ] / Dev. Cell.   DOI   ScienceOn
6 TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility /
[ Basi,G.;E.Schmid;K.Maundrell ] / Gene   DOI   ScienceOn
7 Comparison of Schizosaccharomyces pombe expression systems /
[ Forsburg,S.L. ] / Nucleic Acids Res.   DOI   ScienceOn
8 RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains /
[ Pritchard,C.E.;M.Fornerod;L.H.Kasper;J.M. van Deursen ] / J. Cell. Biol.   DOI   ScienceOn
9 Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export /
[ Strasser,K.;E.Hurt ] / EMBO J.   DOI   ScienceOn
10 The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex /
[ Strawn,L.A.;T.Shen;S.R.Wente ] / J. Biol. Chem.   DOI   ScienceOn
11 Genetic approaches to nuclear pore structure and function /
[ Doye,V.;E.C.Hurt ] / Trends Genet.   DOI   ScienceOn
12 An extensive network of coupling among gene expression machines /
[ Maniatis,T.;R.Reed ] / Nature   DOI   ScienceOn
13 Elf1p, a member of the ABC class of ATPases, functions as a mRNA export factor in Schizosacchromyces pombe /
[ Kozak,L.;G.Gopal;J.H.Yoon;Z.E.Sauna;S.V.Ambudkar;A.G.Thakurta;R.Dhar ] / J. Biol. Chem.   DOI   ScienceOn
14 The nuclear pore complex: from molecular architecture to functional dynamics /
[ Stoffler,D.;B.Fahrenkrog;U.Aebi ] / Curr. Opin. Cell Biol.   DOI   ScienceOn
15 /
[ Alfa,C.;P.Fantes;J.Hyams;M.Mcleod;E.Warbrick ] / Experiments with fission yeast
16 Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking /
[ Fabre,E.;E.Hurt ] / Annu. Rev. Genet.   DOI   ScienceOn
17 Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export /
[ Strasser,K.;J.Bassler;E.Hurt ] / J. Cell. Biol.   DOI   ScienceOn
18 Nuclear RNA export /
[ Stutz,F.;M.Rosbash ] / Genes Dev.   DOI   ScienceOn
19 Thiamine-repressible expression vectors pREP and pRIP for fission yeast /
[ Maundrell,K. ] / Gene   DOI   ScienceOn
20 Crp79p, like Mex67p, is an auxiliary mRNA export factor in Schizosaccharomyces pombe /
[ Thakurta,A.G.;W.A.Whalen;J.H.Yoon;A.Bharathi;L.Kozak;C.Whiteford;D.C.Love;J.A.Hanover;R.Dhar ] / Mol. Biol. Cell   DOI   ScienceOn
21 Nuclear export of mRNA /
[ Zenklusen,D.;F.Stutz ] / FEBS Lett.   DOI
22 Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores /
[ Segref,A.;K.Sharma;V.Doye;A.Hellwig;J.Huber;R.Luhrmann;E.Hurt ] / EMBO J.   DOI   ScienceOn
23 A mutation in the Schizosaccharomyces pombe rae1 gene causes defects in poly(A)+ RNA export and in the cytoskeleton /
[ Brown,J.A.;A.Bharathi;A.Ghosh;W.Whalen;E.Fitzgerald;R.Dhar ] / J. Biol. Chem.   DOI   ScienceOn
24 The nuclear pore complex as a transport machine /
[ Rout,M.P.;J.D.Aitchison ] / J. Biol. Chem.   DOI   ScienceOn
25 Molecular genetic analysis of fission yeast Schizosaccharomyces pombe /
[ Moreno,S.;A.Klar;P.Nurse ] / Methods Enzymol.   DOI
26 Nucleocytoplasmic transport: cargo trafficking across the border /
[ Weis,K. ] / Curr. Opin. Cell Biol.   DOI   ScienceOn
27 Messenger-RNA-binding proteins and the messages they carry /
[ Dreyfuss,G.;V.N.Kim;N.Kataoka ] / Nat. Rev. Mol. Cell. Biol.   DOI   ScienceOn
28 Regulation of mRNA export by nutritional status in fission yeast /
[ Whalen,W.A.;J.H.Yoon;R.Shen;R.Dhar ] / Genetics
29 Regulating Access to the Genome. Nucleocytoplasmic Transport throughout the Cell Cycle /
[ Weis,K. ] / Cell   DOI   ScienceOn
30 Mex67p of Schizosaccharomyces pombe interacts with Rae1p in mediating mRNA export /
[ Yoon,J.H.;D.C.Love;A.Guhathakurta;J.A.Hanover;R.Dhar ] / Mol. Cell Biol.   DOI   ScienceOn
31 Nucleocytoplasmic transport enters the atomic age /
[ Conti,E.;E.Izaurralde ] / Curr. Opin. Cell. Biol.   DOI   ScienceOn
32 Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p /
[ Strasser,K.;E.Hurt ] / Nature   DOI   ScienceOn
33 Nuclear pores and nuclear assembly /
[ Vasu,S.K.;D.J.Forbes ] / Curr. Opin. Cell Biol.   DOI   ScienceOn
34 Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA /
[ Amberg,D.C.;A.L.Goldstein;C.N.Cole ] / Genes Dev.   DOI   ScienceOn