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Microscopic Detection of Urinary Tract Infection in Nepalese Patients  

Dhakal, Bijaya-Kumar (Central Department of Microbiology, Tribhuvan University)
Pokhrel, Bharat-Mani (Department of Microbiology, Tribhuvan University)
Joohong Ahnn (Department of Life Science, Kwangju Institute of Science and Technology)
Publication Information
Journal of Microbiology / v.40, no.4, 2002 , pp. 267-273 More about this Journal
Abstract
Urinary tract infection (UTI) is one of the most common domiciliary and nosocomial bacterial infections prevalent in both males and females. UTI is diagnosed on the basis of clinical symptoms, microscopy and culture of urine. In order to evaluate the efficacy of microscopic detection for presumptive diagnosis of UTI we analyzed urine samples of Nepalese patients. We have conducted Gram staining and counting of pus cells, red blood cells (RBC) and epithelial cells. We observed that RBC and epithelial cell counts were not sensitive enough to be used for presumptive diagnosis of UTI. However, pus cell counts as well as Gram stain are sensitive and significant enough to presume UTI. When the Gram stain result was compared with the culture result, it was statistically significant. From this, we suggest that Gram stain of centrifuged urine is a very sensitive screening method to detect bacteriuria. In addition, we found that E. coli was the most predominant microorganism causing UTI and nitrofurantoin was the most effective antibiotic against the isolated urinary pathogens.
Keywords
bacteriuria; urine; Gram stain; pus cells;
Citations & Related Records
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1 Bacteriology of UTI in two general hospital in Hong Kong. /
[ Ling, J.M.;A.F. Cheng;E.M.Ho. ] / Hong Kong Med.J.
2 Evaluation of three bacteriuria screening methods in a clinical research hospital /
[ Wu,T.C.;E.C.Williams,;S.T.Koo;J.D.MacLowry. ] / J.Ciln.Microbiol.
3 Performance standards for antimicrobial disk suscptibility tests. /
[ National Committee on Clinical Laboratory Standards ] / NCCLS document M2-A6
4 Pyuria among chronically incontinent but otherwise asymptomatic nursing home residents. /
[ Ouslander,J.G.;M.Schapira;J.F.Schnelle;S.Finfold ] / J. Am. Geriatr.Soc.
5 Characterization of quinolone-resistant clinical isolates of Escherichia coli in Korea. /
[ Oh,Y.;S.Park;M.Ha;Y.Lee. ] / J.Microbiol.   과학기술학회마을
6 Culture of urine. /
[ Hoeprich,P.D. ] / J. Lab.Clin.Med.
7 Detection of urinary tract infections by rapid methods. /
[ Pezzlo,M. ] / Clin.Microbiol.Rev.
8 Review of norfloxacin in complicated and recurrent urinary tract infections. /
[ Schaeffer,A.J. ] / Eur.Urol.
9 THe effect of age on prevalence of asymptomatic microscopic hematuria. /
[ Froom,P.;M. Gross;J. Ribak ] / Am.J. Clin. Pathol.
10 Rapid diagnostic mathods for predicting urinary infectionin primary health care. /
[ Pallares,J.;J.Casas;A.Guarga ] / Med.Clin.
11 Significance of bacterial and white cell counts in midstream urines. /
[ Little,P.J.;B.A.Peddie;A.R.Sincock. ] / J. Ciln.Pathol.
12 The dysuria-pyuria syndrome. /
[ Komaroff,A.L.;G.Friedland ] / N.Engl. J.Med.   ScienceOn
13 Scope of urinary pathogens isolated in the Public Health Bacteriology Laboratory ,Harare: Antibiotic susceptibility patterns of isolates and incidence of haemolytic bacteria. /
[ Obi,C.L.;A.Tarupiwa;C.Simango. ] / Cent.Afr.J. Med.
14 Urinary examination, its importance in paediatric medicine. /
[ Singh,S. ] / Indian J. of Pediatr.
15 Failure do the urinalysis and quantitative urine culture in diagnosing symptomaic urinary tract infection in patients with long-term urinary catheters. /
[ Steward,D.K.;G.L.Wood;R.L.Cohen. ] / Am.J.Clin.Pathol.
16 Review of urine microscopy for bacteriuria /
[ Jenkins,R.D.;J.P.Fenn;J.M.Matsen ] / JAMA.   ScienceOn
17 Does pyuria indicate unfection in asymptomatic dialysis patients? /
[ Eisinger,R.P.;F.Asghar;C.Kosala;M.P.Weinstein ] / Clin. Nephrol.
18 Simplified technique for detection of significant bacteriuria by microscopic examination of urine. /
[ Cardoso, C.L.;C.B. Muraro;V.L. Siqueira;M.Guilhermentti ] / J. Clin Microbiol.
19 Detection of significant bacteriuria by microscopic examination of urine. /
[ Washington,J.A.;C.M.White;M.Laganiere;L.H.Smith ] / Lab.Med.
20 /
[ Isenberg,H.D. ] / Clinical microbiology procedures handbook.
21 Analysis of pathogens isolated from urinary tract infection in Barbados. /
[ Levett,P.N. ] / West Indian Med.J.
22 Problems of spectrum and bias in evaluating tne efficacy of diagnostic tests. /
[ Ransohoff,D.F.;A.R.Feinstein. ] / N.Engl.J.Med.   ScienceOn
23 Topley and Wilson’s princioles of bacteriology, virology and immunidy,Bacterial diseases,8th ed /
[ Leigh,D.;G.R.Smith;C.S.F.Easman(eds) ] / Urinary-tract infections
24 Screening for asymptomatic bacteria in pregnancy:Urinalysis vs urine culture. /
[ Abyad,A.R. ] / J. Fam.Pract.
25 Urine sediment analysis by the Yellow IRIS automated urinalysis workstation. /
[ Wargotz,E.S.;J.E.Hyde;D.S.Karcher ] / Am.J.Clin.Pathol.
26 Comparative studies on activities of antimicrobial againts against causative organisms isolated from urinary tract infection:1987:Ⅱ.Background of patients. /
[ Kosakai,N.;Y.KumamotolT.Hirose. ] / Jpn.J.Antibiot.
27 Bacterial infection and male infertility: Absence of immunuglobulin Awith specificity for common Escherichia coli O-serotypes in seminal fluid of infertile man. /
[ Fowler,J.E.Jr;M.Mariano ] / J.Urol
28 Urinary tract infections in the elderly. /
[ Zilkoski,M.W.;D.R.Smucker. ] / Am.Fam. Physician.
29 Clinical diagnosis and management by laboratory method,18th ed. /
[ Schumann,G.B.;S.C.Schweitzer. ] / Examination of urine
30 /
[ Murray,P.R.;E.J.Baron;M.A.Pfaller;F.C.Tenover;R.H.Tolken. ] / Manual of clinical microbiology,(6th ed)
31 /
[ Collee,J.G.;J.P.Duguid;A.G.Fraser;B.P.Marmion ] / Mackie and McCartney practical medical microbiology(13th ed.)
32 /
[ Clarridge,J.E.;M.T.Pezzlo;K.L.Vosti;(Coordinating ed) ] / Cumitech 2A, laboratory disgnosis of urinary infections.
33 Significance of low-count bacteriuria. /
[ Pezzlo,M. ] / Clin.Microbiol.Newsl.   ScienceOn
34 Eliminating unnecessary urine microscopy: Results and performance characteristics of an algorithm based on chemical reagent strip testing. /
[ Wenz,B;J.A.Lampasso. ] / Am.J.Clin.Pathol.
35 Urine screen for bacteriuria in symptomatic pediatric outpatients. /
[ Weinberg,A.G.;V.N.Gan. ] / Pediatr.Infect.Dis.J.   ScienceOn
36 Bioluminescence screening for bacteriuria. /
[ Kollbeck,J.C.;R.A.Padgett;E.G.Estevez;L.J.Harrell ] / J. Clin. Microbiol.
37 /
[ Baron,E.J.;L.R. Peterson;S.M.Finegold. ] / Bailey and Scott’s diagnostic microbiology,9th ed.
38 Review of norfloxacin in lower urinary tract infections. /
[ Miano,L.;S.Gildoni;A.Tubaro. ] / Eur.Urol.
39 Medical laboratory manual for tropical countries. /
[ Cheesbrough,M. ] / Microbiology 1st ELBS ed.
40 Laboratory techniques for detection by rapid methods. /
[ Pollock,H.M. ] / Am. J.Med.