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Isolation, Identification and Characterization of Ynncomycin-Resistant Enterococci from Raw Milk  

Ha, Nam-Joo (Department of Pharmacy, Sahmyook University, Seoul 139-742)
Cho, Sung-Sook (Department of Food Science, Sahmyook College, Seoul 139-742)
Kim, Bong-Su (Laboratory of Antimicrobial Resistant Pathogens, National Institute of Health, Seoul 122-71)
Publication Information
Journal of Microbiology / v.40, no.2, 2002 , pp. 170-172 More about this Journal
Abstract
To determine the occurrence of vancomycin-resistant Enterococci in a raw milk sample, raw milk samples were examined for a period of 6 months. Enterococci were isolated directly from Enterococcal selective agar plates supplemented with 2 mg of vancomycin per liter, Nineteen strains were selected and identified by applying the Vitek system. To determine resistance patterns, 19 isolates were tested with vancomycin and teicoplanin. Vancomycin-resistant Enterococci were genotyped by using a PCR analysis and 5 out of 19 isolates were of the VanC type.
Keywords
vancomycin-resistan; , enterococci; Vitek system; PCR analysis;
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